OBJECTIVE Tumor resistant is the main cause leading to treatment failure.Tumor resistant is related to many aspect of tumor.Jaridon 6 is a new sesquiterpene come from Rabdosia rubescens extacted by our research team p...OBJECTIVE Tumor resistant is the main cause leading to treatment failure.Tumor resistant is related to many aspect of tumor.Jaridon 6 is a new sesquiterpene come from Rabdosia rubescens extacted by our research team previously,have been tested having more obvious advantage than other anti-cancer drugs on tumor-resistant cells including a PTX-resistant gastric cancer cell.But how does the product work?In this paper,we have researched the effect and specific mechanism of Jaridon 6 on gastric-resistant.METHODS In the test,we have adopted vivo and vitro tests to identify the effect of Jaridon 6 on different gastric cancer-resistant cell.The MTT results,Flow test,Western blotting and nude test all verified the anti-cancer of Jaridon 6 on MGC803/PTX gastric resistant cell.We have also used scarifica⁃tion test and transwell tests and Western blotting to testify the inhibition action on EMT by Jaridon 6.The TEM and immu⁃nofluorescence tests have proved the induction autophagy of Jaridon 6.Immunohistochemical tests have been taken to assure the action of Jaridon 6 on EMT,autophagy and PI3K pathway in vivo.RESULTS Jaridon 6 could inhibite the gastric cancer resistant cell in vivo and in vitro.From the mechanism,the inhibiter of 3-MA or LY2940004 have proved the action on PI3K pathway and autophagy by Jaridon 6.Jaridon 6 could induce autophagy in tumor-resistant cells by inhibiting PI3K/AKT pathway.Except that,Jaridon 6 could inhibite the EMT of gastric cancer resistant cell.CONCLUSION Jaridon 6 could be considered for curing gastric cancer resistance,especially for PTX-resistance.Jaridon 6 is a natural autophagy inducer.展开更多
文摘OBJECTIVE Tumor resistant is the main cause leading to treatment failure.Tumor resistant is related to many aspect of tumor.Jaridon 6 is a new sesquiterpene come from Rabdosia rubescens extacted by our research team previously,have been tested having more obvious advantage than other anti-cancer drugs on tumor-resistant cells including a PTX-resistant gastric cancer cell.But how does the product work?In this paper,we have researched the effect and specific mechanism of Jaridon 6 on gastric-resistant.METHODS In the test,we have adopted vivo and vitro tests to identify the effect of Jaridon 6 on different gastric cancer-resistant cell.The MTT results,Flow test,Western blotting and nude test all verified the anti-cancer of Jaridon 6 on MGC803/PTX gastric resistant cell.We have also used scarifica⁃tion test and transwell tests and Western blotting to testify the inhibition action on EMT by Jaridon 6.The TEM and immu⁃nofluorescence tests have proved the induction autophagy of Jaridon 6.Immunohistochemical tests have been taken to assure the action of Jaridon 6 on EMT,autophagy and PI3K pathway in vivo.RESULTS Jaridon 6 could inhibite the gastric cancer resistant cell in vivo and in vitro.From the mechanism,the inhibiter of 3-MA or LY2940004 have proved the action on PI3K pathway and autophagy by Jaridon 6.Jaridon 6 could induce autophagy in tumor-resistant cells by inhibiting PI3K/AKT pathway.Except that,Jaridon 6 could inhibite the EMT of gastric cancer resistant cell.CONCLUSION Jaridon 6 could be considered for curing gastric cancer resistance,especially for PTX-resistance.Jaridon 6 is a natural autophagy inducer.
