本文建立了以超高效液相色谱串联质谱法(UPLC-MS)测定低聚原花青素(OPCs)的方法。样品中原花青素在含高铁离子的酸性乙醇溶液中经密闭高温反应后生成氰定离子,通过含1%甲酸,28%水甲醇组成的流动相(72∶28,v/v)经Zorbax Eclipse Plus C1...本文建立了以超高效液相色谱串联质谱法(UPLC-MS)测定低聚原花青素(OPCs)的方法。样品中原花青素在含高铁离子的酸性乙醇溶液中经密闭高温反应后生成氰定离子,通过含1%甲酸,28%水甲醇组成的流动相(72∶28,v/v)经Zorbax Eclipse Plus C18反相色谱柱(2.1 mm×50 mm,1.8μm)分离,采用大气压电喷雾离子源(ESI)在选择离子监测(SIM)模式下以质荷比为287(m/z)的氰定正离子进行检测,并以美国药典(USP)低聚原花青素标准品进行定量。结果显示原花青素在0.2×10^(-6)~2×10^(-6) g/mL的浓度范围内呈良好线性关系,相关系数大于0.999,三个不同浓度水平的平均加标回收率在98%~104%之间,相对标准偏差(RSD)小于5%,此方法具有较高的精密度和准确度。该液质联用方法同时对比了不同标准品之间的差异,当采用低聚原花青素标准品时将有助于保证产品中原花青素测定结果的一致性。展开更多
Based on the analysis of the urban landscape and its composition,main problems of urban landscape at the early stage of urbanization were summarized,including more attention to the technical content of urban landscape...Based on the analysis of the urban landscape and its composition,main problems of urban landscape at the early stage of urbanization were summarized,including more attention to the technical content of urban landscape,valuing transformation but belittling protection,emphasizing uniformity,ignoring the diversity,lack of scientific planning,and neglecting the value orientation. It is expected that the construction of urban landscape may return to its aesthetic value,practical value,ecological value and other aspects of value orientation. It is believed that the urban landscape is both a type of perception and experience. It can not only maintain the cultural heritage and the evolution of civilization,but also maintain the integration of the biological and physical environment,play the role of diversification in the urban public open space,and undertake social functions such as ecological,disaster prevention,waterlogging,and water conservancy at the same time of protecting and lighting the environment.展开更多
基金Sponsored by Project of National Social Science Foundation(2014BZX084)
文摘Based on the analysis of the urban landscape and its composition,main problems of urban landscape at the early stage of urbanization were summarized,including more attention to the technical content of urban landscape,valuing transformation but belittling protection,emphasizing uniformity,ignoring the diversity,lack of scientific planning,and neglecting the value orientation. It is expected that the construction of urban landscape may return to its aesthetic value,practical value,ecological value and other aspects of value orientation. It is believed that the urban landscape is both a type of perception and experience. It can not only maintain the cultural heritage and the evolution of civilization,but also maintain the integration of the biological and physical environment,play the role of diversification in the urban public open space,and undertake social functions such as ecological,disaster prevention,waterlogging,and water conservancy at the same time of protecting and lighting the environment.
