Former studies show that the Muztag ophiolite, outcropped in the East Kunlun area of Xinjiang, formed in a supra-subduction zone environment. This study is to gain more information about the type of subduetion zone. T...Former studies show that the Muztag ophiolite, outcropped in the East Kunlun area of Xinjiang, formed in a supra-subduction zone environment. This study is to gain more information about the type of subduetion zone. Through field work, thin section observation and microprobe analysis, petrological and mineralogical characteristics of the metamorphic peridotites of this ophiolite are obtained. Although the olivines of metamorphic peridotites appear in three textures of metamorphic relict, metamorphic recrystallizations and orthopyroxene-melting crystallizations by thin-section observations, they have stable and low Fo range of 87.8- 89.5 by microprobe analysis. The orthopyroxenes show metamorphic relict and melting relict textures, with a low En of 88-90 and a wide range of Al2O3 content, from 2.90 wt% to 5. 13 wt%. The spinels develop anhedral-subhedral textures, with Cr^# （=Cr/（Cr＋AI）） focusing on two ranges of 0. 508-0. 723 and 0. 100-0. 118, respectively. Based on these petrological and mineralogical observations, and combined with the era and tectonic setting for the Muztag ophiolite, it can be concluded that the ophiolite formed in a supra-subduction zone where the oceanic crust subducted down to the continental are with a thick continental crust, and resulted from ocean-continent subducion within the Paleotethyan arehiopelagic ocean in the East Kunlun area of Xinjiang.展开更多
【目的】猪繁殖与呼吸综合征(porcine reproductive and respiratory syndrome,PRRS),俗称"蓝耳病",是由猪繁殖与呼吸综合征病毒(porcine reproductive and respiratory syndrome virus,PRRSV)引起的一种高致死性传染病,对世...【目的】猪繁殖与呼吸综合征(porcine reproductive and respiratory syndrome,PRRS),俗称"蓝耳病",是由猪繁殖与呼吸综合征病毒(porcine reproductive and respiratory syndrome virus,PRRSV)引起的一种高致死性传染病,对世界养猪业造成了巨大的经济损失。由于PRRSV遗传变异性较大,因此国内外并未有理想疫苗能够对此病进行有效防制。Cluster of differentiation 163(CD163)是PRRSV感染猪肺泡巨噬细胞(porcine alveolar macrophage,PAM)的受体之一,研究旨在利用CRISPR/Cas9技术结合体细胞核移植技术制备CD163基因编辑的大白猪。【方法】针对猪CD163基因的第7外显子设计构建CRISPR/Cas9基因编辑载体;转染大白猪胎儿成纤维细胞,获得基因编辑阳性细胞克隆;以基因编辑细胞为核供体、体外成熟的猪卵母细胞为核受体构建克隆胚胎;胚胎移植到受体母猪生产CD163基因编辑猪,并进行后续的扩繁试验。【结果】设计的g RNA能够高效的识别靶位点。对获得的127个细胞单克隆进行PCR和测序显示,共有21个克隆发生突变,其中14个克隆为单等位基因突变或双等位基因杂合突变,7个克隆为双等位基因纯合突变。通过体细胞核移植技术,成功获得了CD163双等位基因编辑的纯合大白猪;并获得首批F1代CD163基因编辑仔猪,目前健康状态良好。随后将有更多的F1代CD163基因编辑猪陆续出生。【结论】制备的无抗性筛选标记的CD163双等位基因编辑猪,能够安全并快速地为培育抗PRRSV新品种猪提供育种材料。展开更多
文摘Former studies show that the Muztag ophiolite, outcropped in the East Kunlun area of Xinjiang, formed in a supra-subduction zone environment. This study is to gain more information about the type of subduetion zone. Through field work, thin section observation and microprobe analysis, petrological and mineralogical characteristics of the metamorphic peridotites of this ophiolite are obtained. Although the olivines of metamorphic peridotites appear in three textures of metamorphic relict, metamorphic recrystallizations and orthopyroxene-melting crystallizations by thin-section observations, they have stable and low Fo range of 87.8- 89.5 by microprobe analysis. The orthopyroxenes show metamorphic relict and melting relict textures, with a low En of 88-90 and a wide range of Al2O3 content, from 2.90 wt% to 5. 13 wt%. The spinels develop anhedral-subhedral textures, with Cr^# （=Cr/（Cr＋AI）） focusing on two ranges of 0. 508-0. 723 and 0. 100-0. 118, respectively. Based on these petrological and mineralogical observations, and combined with the era and tectonic setting for the Muztag ophiolite, it can be concluded that the ophiolite formed in a supra-subduction zone where the oceanic crust subducted down to the continental are with a thick continental crust, and resulted from ocean-continent subducion within the Paleotethyan arehiopelagic ocean in the East Kunlun area of Xinjiang.
