Objective:To explore the relationship between the expression of miR-29c and clinicopathological characteristics of gastric cancer and predict the biological functions of target genes of miR-29c.Methods:The expression ...Objective:To explore the relationship between the expression of miR-29c and clinicopathological characteristics of gastric cancer and predict the biological functions of target genes of miR-29c.Methods:The expression and clinical data of microRNA of gastric cancer were downloaded from The Cancer Genome Atlas(TCGA)to analyze the expression of miR-29c in gastric cancer tissues and normal gastric mucosal tissues.Paraffin specimens of gastric cancer and normal tissues were collected to make tissue microarray.The expression of miR-29c in gastric cancer tissues and normal tissues were detected by miRNAscope technique.The relationship between the expression of miR-29c and clinicopathological characteristics of gastric cancer was analyzed by chi-square test.Target genes of miR-29c were predicted by TargetScan,Pictar,miRTarbase and Starbase databases,and enrichment analysis were performed on potential target genens.Results:TCGA analysis showed that the expression of miR-29c was significantly lower in gastric cancer tissue(n=434)than normal tissues(n=41,P<0.001).miRNAscope analysis showed that the expression of miR-29c was significantly lower in gastric cancer tissue than normal tissues(P<0.001),and was significantly associated with TNM staging,lymph node metastasis and histological type of gastric cancer patients(P<0.05).Database predicted 18 potential target genes that were enriched in DNA methyltransferase activity and cancer-associated miRNA signal pathways.Conclusion:miR-29c was expressed at a low level in gastric cancer tissue and associated with TNM staging,lymph node metastasis and histological type,and could become a potential prognosis marker of gastric cancer and a new target of molecular treatment.展开更多
A wake oscillator model is presented for the stream-wise vortex-induced vibration of a circular cylinder in the second excitation region.The near wake dynamics related to the fluctuating nature of alternate vortex she...A wake oscillator model is presented for the stream-wise vortex-induced vibration of a circular cylinder in the second excitation region.The near wake dynamics related to the fluctuating nature of alternate vortex shedding is modeled based on the classical van der Pol equation.An appropriate approach used in cross-flow VIV is developed to estimate the model empirical parameters.The comparison between our calculations and experiments is carried out to validate the proposed model.It is found that the present model results agree fairly well with the experimental data.展开更多
目的:通过文献回顾和Meta分析,探究胸苷激酶1(thymidine kinase 1,TK1)在肺癌诊断中的价值。方法:首先检索Pubmed、Embase、Cochrane、Web of Science、中国生物医学文献数据库(CBM)、中国知网、万方等数据库,并获取截止日期在2022年06...目的:通过文献回顾和Meta分析,探究胸苷激酶1(thymidine kinase 1,TK1)在肺癌诊断中的价值。方法:首先检索Pubmed、Embase、Cochrane、Web of Science、中国生物医学文献数据库(CBM)、中国知网、万方等数据库,并获取截止日期在2022年06月之前的文献。由两名研究者单独筛选报道TK1在肺癌诊疗中的相关临床研究,并获取文献全文。其次,通过使用Revman5.3软件对文献进行系统质量评价和偏倚风险评估,并对各协变量之间的一致性进行评价。最后,通过使用Stata16.0软件将灵敏度(sensitivity,Sen)、特异度(specificity,Spe)等指标进行汇总分析。结果:本研究共纳入37篇文献,其中包含3218例肺癌病例和2976例对照病例,在此基础上进行Meta分析,结果显示,TK1单独作为肺癌诊断指标时,Sen=0.63(95%CI:0.57,0.68),Spe=0.88(95%CI:0.84,0.91),阳性似然比(positive likelihood ratio,PLR)为5.34(95%CI:4.02,7.1),阴性似然比(negative likelihood ratio,NLR)为0.42(95%CI:0.37,0.48),诊断比值比(diagnostic odds ratio,DOR)为12.69(95%CI:8.91,18.08),受试者工作曲线下面积(area under the ROC curve,AUC)为0.82(95%CI:0.79,0.85)。TK1、CEA及CYFRA21⁃1作为肺癌联合诊断指标时,Sen=0.82(95%CI:0.76,0.86),Spe=0.78(95%CI:0.46,0.94),PLR=3.69(95%CI:1.28,10.66),NLR=0.24(95%CI:0.18,0.31),DOR=15.59(95%CI:4.56,53.36),AUC=0.84(95%CI:0.80,0.87);联合诊断的Youden指数为0.60,大于TK1单独诊断时的0.55。结论:TK1单独作为肺癌诊断指标时灵敏度偏低,但特异度较高,具有一定的诊断价值;TK1与CEA、CYFRA21⁃1联合诊断肺癌时有更高的灵敏度,诊断真实性高于TK1单独作为诊断指标时,诊断价值更佳。展开更多
基金National Natural Science Foundation of China(82060437)。
文摘Objective:To explore the relationship between the expression of miR-29c and clinicopathological characteristics of gastric cancer and predict the biological functions of target genes of miR-29c.Methods:The expression and clinical data of microRNA of gastric cancer were downloaded from The Cancer Genome Atlas(TCGA)to analyze the expression of miR-29c in gastric cancer tissues and normal gastric mucosal tissues.Paraffin specimens of gastric cancer and normal tissues were collected to make tissue microarray.The expression of miR-29c in gastric cancer tissues and normal tissues were detected by miRNAscope technique.The relationship between the expression of miR-29c and clinicopathological characteristics of gastric cancer was analyzed by chi-square test.Target genes of miR-29c were predicted by TargetScan,Pictar,miRTarbase and Starbase databases,and enrichment analysis were performed on potential target genens.Results:TCGA analysis showed that the expression of miR-29c was significantly lower in gastric cancer tissue(n=434)than normal tissues(n=41,P<0.001).miRNAscope analysis showed that the expression of miR-29c was significantly lower in gastric cancer tissue than normal tissues(P<0.001),and was significantly associated with TNM staging,lymph node metastasis and histological type of gastric cancer patients(P<0.05).Database predicted 18 potential target genes that were enriched in DNA methyltransferase activity and cancer-associated miRNA signal pathways.Conclusion:miR-29c was expressed at a low level in gastric cancer tissue and associated with TNM staging,lymph node metastasis and histological type,and could become a potential prognosis marker of gastric cancer and a new target of molecular treatment.
基金Supported by the Specialized Research Fund for the Doctoral Program of Higher Education of China(SRFDP)(20100032120047)the Independent Innovation Fund of Tianjin University(2010XJ-0098)the National Natural Science Foundation of China(10902112).
文摘A wake oscillator model is presented for the stream-wise vortex-induced vibration of a circular cylinder in the second excitation region.The near wake dynamics related to the fluctuating nature of alternate vortex shedding is modeled based on the classical van der Pol equation.An appropriate approach used in cross-flow VIV is developed to estimate the model empirical parameters.The comparison between our calculations and experiments is carried out to validate the proposed model.It is found that the present model results agree fairly well with the experimental data.
文摘目的:通过文献回顾和Meta分析,探究胸苷激酶1(thymidine kinase 1,TK1)在肺癌诊断中的价值。方法:首先检索Pubmed、Embase、Cochrane、Web of Science、中国生物医学文献数据库(CBM)、中国知网、万方等数据库,并获取截止日期在2022年06月之前的文献。由两名研究者单独筛选报道TK1在肺癌诊疗中的相关临床研究,并获取文献全文。其次,通过使用Revman5.3软件对文献进行系统质量评价和偏倚风险评估,并对各协变量之间的一致性进行评价。最后,通过使用Stata16.0软件将灵敏度(sensitivity,Sen)、特异度(specificity,Spe)等指标进行汇总分析。结果:本研究共纳入37篇文献,其中包含3218例肺癌病例和2976例对照病例,在此基础上进行Meta分析,结果显示,TK1单独作为肺癌诊断指标时,Sen=0.63(95%CI:0.57,0.68),Spe=0.88(95%CI:0.84,0.91),阳性似然比(positive likelihood ratio,PLR)为5.34(95%CI:4.02,7.1),阴性似然比(negative likelihood ratio,NLR)为0.42(95%CI:0.37,0.48),诊断比值比(diagnostic odds ratio,DOR)为12.69(95%CI:8.91,18.08),受试者工作曲线下面积(area under the ROC curve,AUC)为0.82(95%CI:0.79,0.85)。TK1、CEA及CYFRA21⁃1作为肺癌联合诊断指标时,Sen=0.82(95%CI:0.76,0.86),Spe=0.78(95%CI:0.46,0.94),PLR=3.69(95%CI:1.28,10.66),NLR=0.24(95%CI:0.18,0.31),DOR=15.59(95%CI:4.56,53.36),AUC=0.84(95%CI:0.80,0.87);联合诊断的Youden指数为0.60,大于TK1单独诊断时的0.55。结论:TK1单独作为肺癌诊断指标时灵敏度偏低,但特异度较高,具有一定的诊断价值;TK1与CEA、CYFRA21⁃1联合诊断肺癌时有更高的灵敏度,诊断真实性高于TK1单独作为诊断指标时,诊断价值更佳。
