Objective To investigate the optimal timing for the repair of persistent incomplete facial paralysis by hypoglossal-facial ‘side＇-to-side neurorrhaphy in rats. Methods A total of 30 adult rats with crushed and bulld...Objective To investigate the optimal timing for the repair of persistent incomplete facial paralysis by hypoglossal-facial ‘side＇-to-side neurorrhaphy in rats. Methods A total of 30 adult rats with crushed and bulldog-clamped facial nerve injury were randomly divided into 5 groups（n = 6 each） that were subjected to injury without nerve repair or with immediate repair, 2-week-delayed repair, 4-week-delayed repair, or 8-week-delayed repair. Three months later, the effects of repair in each rat were evaluated by facial symmetry assessment, electrophysiological examination, retrograde labeling, and axon regeneration measurement. Results At 3 months after injury, the alpha angle significantly increased in the group of rats with 4-week-delayed repair compared with the other four groups. Upon stimulation of the facial nerve or Pre degenerated nerve, the muscle action potentials MAPs were recorded in the whisker pad muscle, and the MAP amplitude and area under the curve in the 4-week-delayed repair group were significantly augmented at 3 months post-injury. Similarly, the number of retrograde-labeled motor neurons in the facial and hypoglossal nuclei was quantified to be significantly greater in the 4-week-delayed repair group than in the other groups, and a large number of regenerated axons was also observed. Conclusion The results of this study demonstrated that hemi HN-FN neurorrhaphy performed 4 weeks after facial nerve injury was most effective in terms of the functional recovery of axonal regeneration and activation of facial muscles.展开更多
On December 21, 20：10, a stray dog consecutively attacked 10 people in Lengshui Village, Ningyuan County, Yongzhou City, Hunan Province, China. The dog was killed by the local CDC staff and vicinity villager, its bra...On December 21, 20：10, a stray dog consecutively attacked 10 people in Lengshui Village, Ningyuan County, Yongzhou City, Hunan Province, China. The dog was killed by the local CDC staff and vicinity villager, its brain tissue sample was taken within 24 h. The epidemic focus was disinfected and the injured people received post exposure prophylaxis （PEP）. Pathogens were detected in the tissue sample by the provincial CDC. The immunity and safety of rabies vaccine were assayed after PEP, the injured people were regularly followed up in the following 2 y and 6 mon.展开更多
基金supported by the Basic-Clinical scientific research cooperation fund of Capital Medical University[Grant No.14JL49]+1 种基金the National Natural Science Foundation of China[Grant No.31440051]Special fund for scientific research on health development in the capital[Grant No.2014-2-1073]
文摘Objective To investigate the optimal timing for the repair of persistent incomplete facial paralysis by hypoglossal-facial ‘side＇-to-side neurorrhaphy in rats. Methods A total of 30 adult rats with crushed and bulldog-clamped facial nerve injury were randomly divided into 5 groups（n = 6 each） that were subjected to injury without nerve repair or with immediate repair, 2-week-delayed repair, 4-week-delayed repair, or 8-week-delayed repair. Three months later, the effects of repair in each rat were evaluated by facial symmetry assessment, electrophysiological examination, retrograde labeling, and axon regeneration measurement. Results At 3 months after injury, the alpha angle significantly increased in the group of rats with 4-week-delayed repair compared with the other four groups. Upon stimulation of the facial nerve or Pre degenerated nerve, the muscle action potentials MAPs were recorded in the whisker pad muscle, and the MAP amplitude and area under the curve in the 4-week-delayed repair group were significantly augmented at 3 months post-injury. Similarly, the number of retrograde-labeled motor neurons in the facial and hypoglossal nuclei was quantified to be significantly greater in the 4-week-delayed repair group than in the other groups, and a large number of regenerated axons was also observed. Conclusion The results of this study demonstrated that hemi HN-FN neurorrhaphy performed 4 weeks after facial nerve injury was most effective in terms of the functional recovery of axonal regeneration and activation of facial muscles.
基金supported by the National Department Public Benefit Research Foundation(201103032)
文摘On December 21, 20：10, a stray dog consecutively attacked 10 people in Lengshui Village, Ningyuan County, Yongzhou City, Hunan Province, China. The dog was killed by the local CDC staff and vicinity villager, its brain tissue sample was taken within 24 h. The epidemic focus was disinfected and the injured people received post exposure prophylaxis （PEP）. Pathogens were detected in the tissue sample by the provincial CDC. The immunity and safety of rabies vaccine were assayed after PEP, the injured people were regularly followed up in the following 2 y and 6 mon.
