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降解甲醛微生物的分离鉴定及其降解与代谢特性研究 被引量:1
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作者 王茹 冯永 +12 位作者 李昆志 王晋 许永 田丽梅 缪恩铭 耿咏勤 蒋次清 陈建华 魏玉玲 唐萍 李雪梅 陈丽梅 张承明 《江西农业学报》 CAS 2020年第1期62-71,共10页
首先对新分离的可高效降解甲醛的两株菌株R1和Y1的形态学特征、生理生化特征及16s rRNA序列等进行了系统研究;随后通过测定R1和Y1在液体培养过程中甲醛浓度的变化,确定了它们降解溶液中甲醛的能力。结果表明:菌株R1属于甲基杆状菌属(Met... 首先对新分离的可高效降解甲醛的两株菌株R1和Y1的形态学特征、生理生化特征及16s rRNA序列等进行了系统研究;随后通过测定R1和Y1在液体培养过程中甲醛浓度的变化,确定了它们降解溶液中甲醛的能力。结果表明:菌株R1属于甲基杆状菌属(Methylobacterium),菌株Y1为蜡样芽孢杆菌(Bacillus cereus);当甲醛浓度分别为4、8、10、15 mmol/L时,菌株R1在72 h时的甲醛降解率分别为100%、100%、91%、70%,菌株Y1的甲醛降解率分别为100%、84%、62%、36%;在20 mmol/L甲醛浓度下,菌株R1依然可以存活,72 h时其对甲醛的降解率为64%。13C NMR代谢谱进一步分析表明,菌株R1的主要代谢产物为[3-13C]丝氨酸、[3,4-13C]苹果酸、H13COOH甲酸、[2-13C]甘氨酸、3-磷酸甘油酸和磷酸烯醇式丙酮酸;菌株Y1的主要代谢产物为[3-13C]Ser、PEP、[2-13C]Gly和谷胱甘肽;菌株R1和Y1在代谢甲醛的过程中都可以产生大量的甲酸,且能将甲酸分泌到处理液中。 展开更多
关键词 甲醛 甲基杆菌 蜡样芽孢杆菌 甲醛降解率 代谢产物
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Changes in Soil Physicochemical Properties Following Land Use Change from Paddy Fields to Greenhouse and Upland Fields in the Southeastern Basin of Dianchi Lake,Yunnan Province,China 被引量:4
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作者 N.MORITSUKA T.NISHIKAWA +4 位作者 S.YAMAMOTO N.MATSUI H.INOUE li kun-zhi T.INAMURA 《Pedosphere》 SCIE CAS CSCD 2013年第2期169-176,共8页
Paddy fields in the southeastern basin of Dianchi Lake have rapidly changed to greenhouses since 1999. A total of 61 surface soil samples, including 43 greenhouse soils, 12 upland soils, and 6 paddy soils, were collec... Paddy fields in the southeastern basin of Dianchi Lake have rapidly changed to greenhouses since 1999. A total of 61 surface soil samples, including 43 greenhouse soils, 12 upland soils, and 6 paddy soils, were collected from a flat lowland area mainly used for agricultural production fields in the southeastern basin of Dianchi Lake. Analyses of the soil samples indicated that the greenhouse soils were characterized by a lower organic matter content, lower pH, and higher soluble nutrients than the paddy soils in the area. The lower organic matter content of the greenhouse soils was ascribed to environmental or management factors rather than the clay content of the soil. Accumulation of soluble nutrients, especially inorganic N, was due to over-application of fertilizers, which also caused soil acidification. The average amount of readily available N, P, and K accumulated in the greenhouse soils was estimated to be equal to or higher than the annual input of these nutrients as a fertilizer, indicating that a reduction in fertilizer application was possible and recommended. In contrast, a very low available Si content was observed in the paddy soils, suggesting the need for Si application for rice production. 展开更多
关键词 土壤理化性质 温室土壤 水田土壤 土地利用变化 盆地 滇池 土壤有机质含量 云南省
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