[ Objective] The research aimed to study the low-cost medium instead of MRS to produce lactobacillus suspension and to provide a theoretical basis for the large-scale production of stalk fermented by lactobacillus. [ ...[ Objective] The research aimed to study the low-cost medium instead of MRS to produce lactobacillus suspension and to provide a theoretical basis for the large-scale production of stalk fermented by lactobacillus. [ Method] Different concentrations of glucose and sodium chloride were added into the cooking liquor of maize flour, and lactobacillus was inoculated to ferment the stalk. The changes of pH were measured and compared with MRS medium. The optimum low-cost medium for producing lactobacillus and fermenting stalk was selected to ferment stalk. And the feeding experiment of cattle was made with fermented stalk and unfermented stalk to study the consumption quantity of different kinds of feeds. [ Resuit] The changes of pH in cornmeal and MRS media had the same tendency, but the decrease of pH in cornmeal medium was faster. The consumed quantity of fermented stalk feed was larger than that of unfermented feeds. [ Conclusion] Cornmeal medium can be used instead of MRS to produce lactobacillus and the production cost decreased more than 80%.展开更多
基金funded by the National Special Research Fund for Public Welfare (Agriculture) of China (200803033B0502)
文摘[ Objective] The research aimed to study the low-cost medium instead of MRS to produce lactobacillus suspension and to provide a theoretical basis for the large-scale production of stalk fermented by lactobacillus. [ Method] Different concentrations of glucose and sodium chloride were added into the cooking liquor of maize flour, and lactobacillus was inoculated to ferment the stalk. The changes of pH were measured and compared with MRS medium. The optimum low-cost medium for producing lactobacillus and fermenting stalk was selected to ferment stalk. And the feeding experiment of cattle was made with fermented stalk and unfermented stalk to study the consumption quantity of different kinds of feeds. [ Resuit] The changes of pH in cornmeal and MRS media had the same tendency, but the decrease of pH in cornmeal medium was faster. The consumed quantity of fermented stalk feed was larger than that of unfermented feeds. [ Conclusion] Cornmeal medium can be used instead of MRS to produce lactobacillus and the production cost decreased more than 80%.
