An efficient plant regeneration protocol was established for castor bean （Ricinus communis L.）, in which 0.3 mg L-1 thidiazuron （TDZ） induced shoot clusters and increased the number of adventitious shoots from hyp...An efficient plant regeneration protocol was established for castor bean （Ricinus communis L.）, in which 0.3 mg L-1 thidiazuron （TDZ） induced shoot clusters and increased the number of adventitious shoots from hypocotyl tissue. Our results showed that treatment under dark conditions significantly promoted the average number of shoots per explant to 37.36±4.54 （with a 6-d treatment）. Modified 1/2 Murashige and Skoog （MS） basal medium supplemented with 440 mg L-1 Ca2＋, 0.2 mg L-1 gibberellic acid and 0.1 mg L-1 TDZ significantly increased shoot elongation rates and lowered vitrification rates. Further- more, 1/2 MS media supplemented with 0.2 mg L-11-naphthaleneacetic acid induced a higher rooting rate compared with other culture conditions.展开更多
目的基于核糖体18S r DNA基因对基氏蠊螨进行分子鉴定。方法采集和分离储藏物样本,进行螨类的形态学鉴定。提取单个螨的基因组DNA,经PCR扩增、克隆和测序获得COⅠ基因和18S r DNA基因,将所获序列进行Blast对比。检索Gen Bank数据库中蠊...目的基于核糖体18S r DNA基因对基氏蠊螨进行分子鉴定。方法采集和分离储藏物样本,进行螨类的形态学鉴定。提取单个螨的基因组DNA,经PCR扩增、克隆和测序获得COⅠ基因和18S r DNA基因,将所获序列进行Blast对比。检索Gen Bank数据库中蠊螨属18S r DNA基因序列,利用Clustal X 1.83软件进行多序列比对,基于MEGA X软件进行序列分析并以邻接法(Neighbor-Joining,NJ)构建系统发育树。结果采集的样本经形态和COⅠ基因双重鉴定为基氏蠊螨。同时,所选取的10个基氏蠊螨的18S r DNA基因序列完全一致,均表现出A/T碱基偏向性,与同属的Blattisocius tarsalis和Blattisocius everti分别有98.73%和98.94%的同源性。基于18S r DNA基因序列的系统进化树显示,基氏蠊螨与Blattisocius tarsalis和Blattisocius everti聚为一支。结论本研究建立了基氏蠊螨基于18S r DNA基因序列的分子鉴定方法,为基氏蠊螨的准确鉴定奠定基础。展开更多
基金supported by the National Natural Science Foundation of China (31260336 and 31460353)
文摘An efficient plant regeneration protocol was established for castor bean （Ricinus communis L.）, in which 0.3 mg L-1 thidiazuron （TDZ） induced shoot clusters and increased the number of adventitious shoots from hypocotyl tissue. Our results showed that treatment under dark conditions significantly promoted the average number of shoots per explant to 37.36±4.54 （with a 6-d treatment）. Modified 1/2 Murashige and Skoog （MS） basal medium supplemented with 440 mg L-1 Ca2＋, 0.2 mg L-1 gibberellic acid and 0.1 mg L-1 TDZ significantly increased shoot elongation rates and lowered vitrification rates. Further- more, 1/2 MS media supplemented with 0.2 mg L-11-naphthaleneacetic acid induced a higher rooting rate compared with other culture conditions.
文摘目的基于核糖体18S r DNA基因对基氏蠊螨进行分子鉴定。方法采集和分离储藏物样本,进行螨类的形态学鉴定。提取单个螨的基因组DNA,经PCR扩增、克隆和测序获得COⅠ基因和18S r DNA基因,将所获序列进行Blast对比。检索Gen Bank数据库中蠊螨属18S r DNA基因序列,利用Clustal X 1.83软件进行多序列比对,基于MEGA X软件进行序列分析并以邻接法(Neighbor-Joining,NJ)构建系统发育树。结果采集的样本经形态和COⅠ基因双重鉴定为基氏蠊螨。同时,所选取的10个基氏蠊螨的18S r DNA基因序列完全一致,均表现出A/T碱基偏向性,与同属的Blattisocius tarsalis和Blattisocius everti分别有98.73%和98.94%的同源性。基于18S r DNA基因序列的系统进化树显示,基氏蠊螨与Blattisocius tarsalis和Blattisocius everti聚为一支。结论本研究建立了基氏蠊螨基于18S r DNA基因序列的分子鉴定方法,为基氏蠊螨的准确鉴定奠定基础。
