In this study,we investigate how Schwann cells and dorsal root ganglion(DRG)neurons response to direct low-intensity pulsed ultrasound(LIPUS)stimuli in vitro.Primary Schwann cells and DRG were isolated from rat sciati...In this study,we investigate how Schwann cells and dorsal root ganglion(DRG)neurons response to direct low-intensity pulsed ultrasound(LIPUS)stimuli in vitro.Primary Schwann cells and DRG were isolated from rat sciatic nerve and spine,respectively.LIPUS with varied dose of intensity(low:250 mW/cm2,medium:500 m W/cm2,high:750 m W/cm2)were applied 5 min per time for every other day,and pro-myelination indicators of Schwann cell as well as neurite outgrowth of dorsal root ganglion neurons were analyzed.Our results demonstrated that LIPUS promoted Schwann cells activity and proliferation from day 3 at the highest intensity,and day 5 at all intensities.In addition,LIPUS boosted pro-myelination activities of Schwann cells,as evidenced by increased cell population that positive for immunohistochemical staining against S100,nerve growth factor receptor(NGFR)p75,glial fibrillary acidic protein(GFAP),myelin protein zero(P0),as well as up-regulation of GFAP,Protein 0,nerve growth factor(NGF),and brain derived neurotrophic factor(BDNF)genes.Furthermore,LIPUS significantly enhanced the neurite outgrowth of DRG,with the highest intensity exhibiting longest neurite outgrowth.Taken together,our results strongly improve the understanding of cellular mechanisms of ultrasonic therapies for peripheral nerve repair.展开更多
目的观察超声造影基线肾皮质血流灌注参数中上升支曲线下面积(wash-in area under curve,iAUC)对重度肾动脉狭窄(renal artery stenosis,RAS)患者支架治疗短期预后的影响。方法回顾性分析2017年10月至2019年12月北京医院收治的82例单侧...目的观察超声造影基线肾皮质血流灌注参数中上升支曲线下面积(wash-in area under curve,iAUC)对重度肾动脉狭窄(renal artery stenosis,RAS)患者支架治疗短期预后的影响。方法回顾性分析2017年10月至2019年12月北京医院收治的82例单侧重度RAS行支架治疗的患者,根据术前超声造影基线患肾皮质血流灌注参数iAUC,分为灌注不良组(iAUC<850.0 dB×s)37例和灌注良好组(iAUC≥850.0 dB×s)45例,比较两组基线和临床影像学资料。随访(11.5±1.7)个月,采用Kaplan-Meier生存曲线和Log-rank检验比较两组不良心肾血管事件发生和高血压治疗情况。结果与灌注良好组比较,灌注不良组高血压病程长,糖尿病患者更多,收缩压、舒张压、24 h平均收缩压、24 h平均舒张压更高,肾小球滤过率更低,RAS程度更重,上升支和下降支曲线下面积、峰值强度更低,平均渡越时间更长,降糖治疗率更高(均P<0.05)。Kaplan-Meier生存曲线和Log-rank检验分析显示,灌注良好组心肾血管事件(HR=0.361,95%CI=0.144~0.907,P=0.012)和肾功能恶化率(HR=0.286,95%CI=0.090~0.914,P=0.035)的发生风险均显著低于灌注不良组。灌注良好组高血压治疗有效率显著高于灌注不良组(93.3%对59.5%,P<0.001),但两组高血压改善率(60.0%对43.2%)和治愈率(28.9%对16.2%)差异无统计学意义(均P>0.05)。结论超声造影基线肾皮质血流灌注不良的重度RAS患者常合并糖尿病,高血压病程更长,肾小球滤过率明显减低和RAS更重,且支架植入术后短期预后不良。展开更多
基金Supported by the National Natural Science Foundation of China(81327003)
文摘In this study,we investigate how Schwann cells and dorsal root ganglion(DRG)neurons response to direct low-intensity pulsed ultrasound(LIPUS)stimuli in vitro.Primary Schwann cells and DRG were isolated from rat sciatic nerve and spine,respectively.LIPUS with varied dose of intensity(low:250 mW/cm2,medium:500 m W/cm2,high:750 m W/cm2)were applied 5 min per time for every other day,and pro-myelination indicators of Schwann cell as well as neurite outgrowth of dorsal root ganglion neurons were analyzed.Our results demonstrated that LIPUS promoted Schwann cells activity and proliferation from day 3 at the highest intensity,and day 5 at all intensities.In addition,LIPUS boosted pro-myelination activities of Schwann cells,as evidenced by increased cell population that positive for immunohistochemical staining against S100,nerve growth factor receptor(NGFR)p75,glial fibrillary acidic protein(GFAP),myelin protein zero(P0),as well as up-regulation of GFAP,Protein 0,nerve growth factor(NGF),and brain derived neurotrophic factor(BDNF)genes.Furthermore,LIPUS significantly enhanced the neurite outgrowth of DRG,with the highest intensity exhibiting longest neurite outgrowth.Taken together,our results strongly improve the understanding of cellular mechanisms of ultrasonic therapies for peripheral nerve repair.
文摘目的观察超声造影基线肾皮质血流灌注参数中上升支曲线下面积(wash-in area under curve,iAUC)对重度肾动脉狭窄(renal artery stenosis,RAS)患者支架治疗短期预后的影响。方法回顾性分析2017年10月至2019年12月北京医院收治的82例单侧重度RAS行支架治疗的患者,根据术前超声造影基线患肾皮质血流灌注参数iAUC,分为灌注不良组(iAUC<850.0 dB×s)37例和灌注良好组(iAUC≥850.0 dB×s)45例,比较两组基线和临床影像学资料。随访(11.5±1.7)个月,采用Kaplan-Meier生存曲线和Log-rank检验比较两组不良心肾血管事件发生和高血压治疗情况。结果与灌注良好组比较,灌注不良组高血压病程长,糖尿病患者更多,收缩压、舒张压、24 h平均收缩压、24 h平均舒张压更高,肾小球滤过率更低,RAS程度更重,上升支和下降支曲线下面积、峰值强度更低,平均渡越时间更长,降糖治疗率更高(均P<0.05)。Kaplan-Meier生存曲线和Log-rank检验分析显示,灌注良好组心肾血管事件(HR=0.361,95%CI=0.144~0.907,P=0.012)和肾功能恶化率(HR=0.286,95%CI=0.090~0.914,P=0.035)的发生风险均显著低于灌注不良组。灌注良好组高血压治疗有效率显著高于灌注不良组(93.3%对59.5%,P<0.001),但两组高血压改善率(60.0%对43.2%)和治愈率(28.9%对16.2%)差异无统计学意义(均P>0.05)。结论超声造影基线肾皮质血流灌注不良的重度RAS患者常合并糖尿病,高血压病程更长,肾小球滤过率明显减低和RAS更重,且支架植入术后短期预后不良。
