Helical strakes have been widely applied for suppressing the vibration of flexible cylinders undergoing vortexshedding in offshore engineering.However,most research works have concerned on the application of helical s...Helical strakes have been widely applied for suppressing the vibration of flexible cylinders undergoing vortexshedding in offshore engineering.However,most research works have concerned on the application of helical strakes for the isolated flexible cylinder subjected to vortex-induced vibration(VIV).The effectiveness of helical strakes attached to side-by-side flexible cylinders in vibration reduction is still unclear.In this paper,the response characteristics of two side-by-side flexible cylinders with and without helical strakes were experimentally investigated in a towing tank.The configuration of the helical strakes used in the experiment had a pitch of 17.5D and a height of 0.25D(where D is the cylinder diameter),which is usually considered the most effective for VIV suppression of isolated marine risers and tendons.The center-to-center distance of the two cylinders was 3.0D.The uniform flow with a velocity ranging from 0.05 m/s to 1.0 m/s was generated by towing the cylinder models along the tank.Experimental results,including the displacement amplitude,the dominant frequency,the dominant mode,and the mean drag force coefficient,were summarized and discussed.For the case where only one cylinder in the two-cylinder system had helical strakes,the experimental results indicated that helical strakes can remarkably reduce the flow-induced vibration(FIV)of the staked cylinder.For the case of two straked cylinders in a side-by-side arrangement,it was found that the performance of helical strakes in suppressing the FIV is as good as that for the isolated cylinder.展开更多
5-Hydroxymethylfurfural （5-HMF）, a water-soluble compound extracted from wine-processed Fructus corni, is a novel hepatic protectant for treating acute liver injury. The present study was designed to investigate the...5-Hydroxymethylfurfural （5-HMF）, a water-soluble compound extracted from wine-processed Fructus corni, is a novel hepatic protectant for treating acute liver injury. The present study was designed to investigate the protective effect of 5-HMF in human L02 hepatocytes injured by D-galactosamine （GAIN） and tumor necrosis factor-α （TNF-α） in vitro and to explore the underlying mechanisms of action. Our results showed that 5-HMF caused significant increase in the viability of L02 cells injured by GalN/TNF-α, in accordance with a dose-dependent decrease in apoptotic cell death confirmed by morphological and flow cytometric analyses. Based on immunofluorescence and Western blot assays, we found that GalN/TNF-α induced ER stress in the cells, as indicated by the disturbance of intracellular Ca^2＋ concentration, the activation of protein kinase RNA （PKR）-like ER kinase （PERK）, phosphorylation of eukaryotic initiation factor 2 alpha （eIF2α）, and expression of ATF4 and CHOP proteins, which was reversed by 5-HMF pre-treatment in a dose-dependent manner. The anti-apoptotic effect of 5-HMF was further evidenced by balancing the expression of Bcl-2 family members. In addition, the knockdown of PERK suppressed the expression of phospho-PERK, phospho-eIF2α, ATF4, and CHOP, resulting in a significant decrease in cell apoptosis after the treatment with GalN/TNF-α. 5-HMF could enhance the effects of PERK knockdown, protecting the cells against the GalN/TNF-α insult. In conclusion, these findings demonstrate that 5-HMF can effectively protect GalN/TNF-α-injured L02 hepatocytes against ER stress-induced apoptosis through the regulation of the PERK- eIF2α signaling pathway, suggesting that it is a possible candidate for liver disease therapy.展开更多
基金the National Natural Science Foundation of China(Grant Nos.51679167,51979193 and 51678548).
文摘Helical strakes have been widely applied for suppressing the vibration of flexible cylinders undergoing vortexshedding in offshore engineering.However,most research works have concerned on the application of helical strakes for the isolated flexible cylinder subjected to vortex-induced vibration(VIV).The effectiveness of helical strakes attached to side-by-side flexible cylinders in vibration reduction is still unclear.In this paper,the response characteristics of two side-by-side flexible cylinders with and without helical strakes were experimentally investigated in a towing tank.The configuration of the helical strakes used in the experiment had a pitch of 17.5D and a height of 0.25D(where D is the cylinder diameter),which is usually considered the most effective for VIV suppression of isolated marine risers and tendons.The center-to-center distance of the two cylinders was 3.0D.The uniform flow with a velocity ranging from 0.05 m/s to 1.0 m/s was generated by towing the cylinder models along the tank.Experimental results,including the displacement amplitude,the dominant frequency,the dominant mode,and the mean drag force coefficient,were summarized and discussed.For the case where only one cylinder in the two-cylinder system had helical strakes,the experimental results indicated that helical strakes can remarkably reduce the flow-induced vibration(FIV)of the staked cylinder.For the case of two straked cylinders in a side-by-side arrangement,it was found that the performance of helical strakes in suppressing the FIV is as good as that for the isolated cylinder.
基金supported by grants from Natural Science Foundation of Jiangsu Higher Education Institutions of China(No.13KJB360010)Jiangsu Collaborative Innovation Center of Traditional Chinese Medicine(TCM) Prevention and Treatment of Tumor(No.012092002002)+1 种基金China and Europe Taking Care of Healthcare Solutions,CHETCH(No.PIRSES-GA-2013-612589)Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)
文摘5-Hydroxymethylfurfural （5-HMF）, a water-soluble compound extracted from wine-processed Fructus corni, is a novel hepatic protectant for treating acute liver injury. The present study was designed to investigate the protective effect of 5-HMF in human L02 hepatocytes injured by D-galactosamine （GAIN） and tumor necrosis factor-α （TNF-α） in vitro and to explore the underlying mechanisms of action. Our results showed that 5-HMF caused significant increase in the viability of L02 cells injured by GalN/TNF-α, in accordance with a dose-dependent decrease in apoptotic cell death confirmed by morphological and flow cytometric analyses. Based on immunofluorescence and Western blot assays, we found that GalN/TNF-α induced ER stress in the cells, as indicated by the disturbance of intracellular Ca^2＋ concentration, the activation of protein kinase RNA （PKR）-like ER kinase （PERK）, phosphorylation of eukaryotic initiation factor 2 alpha （eIF2α）, and expression of ATF4 and CHOP proteins, which was reversed by 5-HMF pre-treatment in a dose-dependent manner. The anti-apoptotic effect of 5-HMF was further evidenced by balancing the expression of Bcl-2 family members. In addition, the knockdown of PERK suppressed the expression of phospho-PERK, phospho-eIF2α, ATF4, and CHOP, resulting in a significant decrease in cell apoptosis after the treatment with GalN/TNF-α. 5-HMF could enhance the effects of PERK knockdown, protecting the cells against the GalN/TNF-α insult. In conclusion, these findings demonstrate that 5-HMF can effectively protect GalN/TNF-α-injured L02 hepatocytes against ER stress-induced apoptosis through the regulation of the PERK- eIF2α signaling pathway, suggesting that it is a possible candidate for liver disease therapy.
