As important yield-related traits,thousand-grain weight(TGW),grain number per spike(GNS)and grain weight per spike(GWS)are crucial components of wheat production.To dissect their underlying genetic basis,a double hapl...As important yield-related traits,thousand-grain weight(TGW),grain number per spike(GNS)and grain weight per spike(GWS)are crucial components of wheat production.To dissect their underlying genetic basis,a double haploid(DH)population comprised of 198 lines derived from 8762/Keyi 5214 was constructed.We then used genechip to genotype the DH population and integrated the yield-related traits TGW,GNS and GWS for QTL mapping.Finally,we obtained a total of 18942 polymorphic SNP markers and identified 41 crucial QTLs for these traits.Three stable QTLs for TGW were identified on chromosomes 2D(QTgw-2D.3 and QTgw-2D.4)and 6A(QTgw-6A.1),with additive alleles all from the parent 8762,explaining 4.81–18.67%of the phenotypic variations.Five stable QTLs for GNS on chromosomes 3D,5B,5D and 6A were identified.QGns-5D.1 was from parent 8762,while the other four QTLs were from parent Keyi 5214,explaining 5.89–7.08%of the GNS phenotypic variations.In addition,a stable GWS genetic locus QGws-4A.3 was detected from the parent 8762,which explained 6.08–6.14%of the phenotypic variations.To utilize the identified QTLs,we developed STARP markers for four important QTLs,Tgw2D.3-2,Tgw2D.4-1,Tgw6A.1 and Gns3D.1.Our results provide important basic resources and references for the identification and cloning of genes related to TGW,GNS and GWS in wheat.展开更多
目的初步阐释木蝴蝶总黄酮抗肝肿瘤的作用机制,为木蝴蝶总黄酮作为抗肝癌药物的开发和临床应用提供实验依据。方法采用二乙基亚硝胺(DEN)诱发大鼠肝癌模型,随机分为空白组,模型组,阳性对照组,木蝴蝶总黄酮高、中、低剂量组,从造模第6周...目的初步阐释木蝴蝶总黄酮抗肝肿瘤的作用机制,为木蝴蝶总黄酮作为抗肝癌药物的开发和临床应用提供实验依据。方法采用二乙基亚硝胺(DEN)诱发大鼠肝癌模型,随机分为空白组,模型组,阳性对照组,木蝴蝶总黄酮高、中、低剂量组,从造模第6周开始给药,每日1次,连续10周。第17周眼球取血,分离各组血浆。采用HPLC-QTOF-MS联用技术分析木蝴蝶总黄酮干预后各组大鼠血浆中代谢物的变化,利用Agilent Mass Profiler Professional 12.6软件结合相关数据库进行主成分分析(PCA)、差异代谢物分析及代谢通路分析。结果与模型组比较,木蝴蝶总黄酮干预后共有285种代谢物发生变化,其中上调的有41种,下调的有244种。主要与色氨酸代谢、丝氨酸代谢、胆汁酸合成、花生四烯酸代谢、亚油酸代谢等途径有关。结论木蝴蝶总黄酮发挥抗肝肿瘤作用可能与调节氨基酸代谢、脂肪酸代谢、胆汁酸合成等代谢途径有关。展开更多
基金the Henan Modern Agricultural Industrial Technology System Construction,China(HARS-22-1-Z7)。
文摘As important yield-related traits,thousand-grain weight(TGW),grain number per spike(GNS)and grain weight per spike(GWS)are crucial components of wheat production.To dissect their underlying genetic basis,a double haploid(DH)population comprised of 198 lines derived from 8762/Keyi 5214 was constructed.We then used genechip to genotype the DH population and integrated the yield-related traits TGW,GNS and GWS for QTL mapping.Finally,we obtained a total of 18942 polymorphic SNP markers and identified 41 crucial QTLs for these traits.Three stable QTLs for TGW were identified on chromosomes 2D(QTgw-2D.3 and QTgw-2D.4)and 6A(QTgw-6A.1),with additive alleles all from the parent 8762,explaining 4.81–18.67%of the phenotypic variations.Five stable QTLs for GNS on chromosomes 3D,5B,5D and 6A were identified.QGns-5D.1 was from parent 8762,while the other four QTLs were from parent Keyi 5214,explaining 5.89–7.08%of the GNS phenotypic variations.In addition,a stable GWS genetic locus QGws-4A.3 was detected from the parent 8762,which explained 6.08–6.14%of the phenotypic variations.To utilize the identified QTLs,we developed STARP markers for four important QTLs,Tgw2D.3-2,Tgw2D.4-1,Tgw6A.1 and Gns3D.1.Our results provide important basic resources and references for the identification and cloning of genes related to TGW,GNS and GWS in wheat.
文摘目的初步阐释木蝴蝶总黄酮抗肝肿瘤的作用机制,为木蝴蝶总黄酮作为抗肝癌药物的开发和临床应用提供实验依据。方法采用二乙基亚硝胺(DEN)诱发大鼠肝癌模型,随机分为空白组,模型组,阳性对照组,木蝴蝶总黄酮高、中、低剂量组,从造模第6周开始给药,每日1次,连续10周。第17周眼球取血,分离各组血浆。采用HPLC-QTOF-MS联用技术分析木蝴蝶总黄酮干预后各组大鼠血浆中代谢物的变化,利用Agilent Mass Profiler Professional 12.6软件结合相关数据库进行主成分分析(PCA)、差异代谢物分析及代谢通路分析。结果与模型组比较,木蝴蝶总黄酮干预后共有285种代谢物发生变化,其中上调的有41种,下调的有244种。主要与色氨酸代谢、丝氨酸代谢、胆汁酸合成、花生四烯酸代谢、亚油酸代谢等途径有关。结论木蝴蝶总黄酮发挥抗肝肿瘤作用可能与调节氨基酸代谢、脂肪酸代谢、胆汁酸合成等代谢途径有关。
