The allelic frequency, the polymorphic information contents (PIC), the number of effective alleles, the heterozygosity, and the genetic distances were studied in three imported meat sheep (Suffolk, Dorset, Texel) ...The allelic frequency, the polymorphic information contents (PIC), the number of effective alleles, the heterozygosity, and the genetic distances were studied in three imported meat sheep (Suffolk, Dorset, Texel) and their F1 crossbred obtained from those crossed with indigenous Small Tail Hun Sheep (Suffolk♂× Small Tail Hun Sheep, SH; Dorset ♂× Small Tail Han Sheep♂, DH; Texel♂× Small Tail Hart Sheep ♀, TH) using six microsatellite DNA loci. The perpormences of three-breed crossbred (Suffolk ♂× DH ♀, Suffolk ♂× TH ♀, Texel ♂× SH ♀, Texel ♂× DH ♀, Dorset ♂× TH ♀, and Dorset ♂× SH ♀ ) were tested. The results indicated that there were genetic polymorphisms at six microsatellite loci in six sheep populations. Six microsatellite loci could be used for genetic diversity evaluation in sheep populations. The order of three-breed heterosis by the analysis of genetic relationship from large to small was Texel ♂× DH ♀, Suffolk ♂× DH ♀, Suffolki ♂× TH ♀, Texel ♂× SH ♀, Dorset ♂×TH ♀, and Dorset ♂× SH ♀, which was in accordance with the testing results on actual heterosis. These results showed that prediction of the best three-breed hybridized combination among sheep breeds by microsatellite DNA polymorphism was feasible, which will have an important value on the reasonable utilization of introduced meat sheep and sheep breeding in our country in the future.展开更多
报道香委陵菜(Potentilla suavis)的叶绿体全基因组。香委陵菜的叶绿体基因组为环状分子,全长155044bp。基因组呈现典型的四分体结构:包括一个大单拷贝区(large single copy,LSC)、一个小单拷贝区(small single copy,SSC)和1对被大...报道香委陵菜(Potentilla suavis)的叶绿体全基因组。香委陵菜的叶绿体基因组为环状分子,全长155044bp。基因组呈现典型的四分体结构:包括一个大单拷贝区(large single copy,LSC)、一个小单拷贝区(small single copy,SSC)和1对被大小单拷贝区隔开的反向重复区(inverted repeats,IRa和IRb),长度分别为84334bp、18452bp和26129bp。香委陵菜叶绿体基因组共有129个基因,包括84个蛋白质编码基因,37个转运RNA(tRNA)基因和8个核糖体RNA(rRNA)基因,其中17个基因含有2个拷贝。系统发育分析表明,在当前类群取样下香委陵菜与紫花委陵菜(Potentilla purpurea)为姐妹群。展开更多
基金supported by the Key Project of Ministry of Science and Technology of China(2008BADB2B04-9)the Key Project of Hebei Province,China(07220401D-2)
文摘The allelic frequency, the polymorphic information contents (PIC), the number of effective alleles, the heterozygosity, and the genetic distances were studied in three imported meat sheep (Suffolk, Dorset, Texel) and their F1 crossbred obtained from those crossed with indigenous Small Tail Hun Sheep (Suffolk♂× Small Tail Hun Sheep, SH; Dorset ♂× Small Tail Han Sheep♂, DH; Texel♂× Small Tail Hart Sheep ♀, TH) using six microsatellite DNA loci. The perpormences of three-breed crossbred (Suffolk ♂× DH ♀, Suffolk ♂× TH ♀, Texel ♂× SH ♀, Texel ♂× DH ♀, Dorset ♂× TH ♀, and Dorset ♂× SH ♀ ) were tested. The results indicated that there were genetic polymorphisms at six microsatellite loci in six sheep populations. Six microsatellite loci could be used for genetic diversity evaluation in sheep populations. The order of three-breed heterosis by the analysis of genetic relationship from large to small was Texel ♂× DH ♀, Suffolk ♂× DH ♀, Suffolki ♂× TH ♀, Texel ♂× SH ♀, Dorset ♂×TH ♀, and Dorset ♂× SH ♀, which was in accordance with the testing results on actual heterosis. These results showed that prediction of the best three-breed hybridized combination among sheep breeds by microsatellite DNA polymorphism was feasible, which will have an important value on the reasonable utilization of introduced meat sheep and sheep breeding in our country in the future.
文摘报道香委陵菜(Potentilla suavis)的叶绿体全基因组。香委陵菜的叶绿体基因组为环状分子,全长155044bp。基因组呈现典型的四分体结构:包括一个大单拷贝区(large single copy,LSC)、一个小单拷贝区(small single copy,SSC)和1对被大小单拷贝区隔开的反向重复区(inverted repeats,IRa和IRb),长度分别为84334bp、18452bp和26129bp。香委陵菜叶绿体基因组共有129个基因,包括84个蛋白质编码基因,37个转运RNA(tRNA)基因和8个核糖体RNA(rRNA)基因,其中17个基因含有2个拷贝。系统发育分析表明,在当前类群取样下香委陵菜与紫花委陵菜(Potentilla purpurea)为姐妹群。