为研究贮藏温度对脆皮肠和方片火腿品质的影响,测定其在2℃和8℃恒定温度、2/8℃(前15 d 2℃,后15 d 8℃)和8/2℃(前15 d 8℃,后15 d 2℃)分段低温贮藏条件下放置30 d的理化及微生物指标变化。结果表明:脆皮肠和方片火腿的指标变化趋势...为研究贮藏温度对脆皮肠和方片火腿品质的影响,测定其在2℃和8℃恒定温度、2/8℃(前15 d 2℃,后15 d 8℃)和8/2℃(前15 d 8℃,后15 d 2℃)分段低温贮藏条件下放置30 d的理化及微生物指标变化。结果表明:脆皮肠和方片火腿的指标变化趋势大体相同,贮藏温度越高,硫代巴比妥酸(thiobarbituric acid,TBA)值和挥发性盐基氮(total volatile basic nitrogen,TVB-N)含量越大,pH值越小;2℃恒温贮藏可以有效减少菌落总数,TVB-N含量、TBA值、pH值、质构变化缓慢,贮藏结束时,脆皮肠和方片火腿的菌落总数分别为3.24、2.94(lg(CFU/g)),TVB-N含量分别为57.64、66.53 mg/100 g,TBA值分别为0.15、0.14 mg/kg,pH值分别为5.98和5.91。8℃最不利于贮藏,分段贮藏组中2/8℃条件下贮藏样品的品质较好,恒定低温2℃或者前期低温贮藏更有利于保持产品品质稳定,延长肉制品货架期。展开更多
Exosomes,extracellular vesicles(EVs)that play crucial roles in biological processes,have emerged as attractive targets for noninvasive disease diagnosis and monitoring.Mass spectrometry(MS)offers high sensitivity,high...Exosomes,extracellular vesicles(EVs)that play crucial roles in biological processes,have emerged as attractive targets for noninvasive disease diagnosis and monitoring.Mass spectrometry(MS)offers high sensitivity,high throughput,and excellent qualification and quantification capacity,which is the ideal approach for exosome analysis.Nanomaterials’unique physiochemical properties,controllable morphology,and large surface area make them promising in biological sample pretreatment and detection.They contributed diverse functions to the exosome MS analysis,encompassing substrates for exosome isolation and cargos enrichment,matrices for laser desorption/ionization(LDI),read-out signals and supporters for signal amplification strategies,etc.In this review,recent progress in the applications of nanomaterial in the exosome MS analysis was summarized and a comprehensive discussion on the challenges and perspectives was proposed for the development of an advancing analysis approach of exosomes for the accurate diagnosis and monitoring of diseases.展开更多
文摘为研究贮藏温度对脆皮肠和方片火腿品质的影响,测定其在2℃和8℃恒定温度、2/8℃(前15 d 2℃,后15 d 8℃)和8/2℃(前15 d 8℃,后15 d 2℃)分段低温贮藏条件下放置30 d的理化及微生物指标变化。结果表明:脆皮肠和方片火腿的指标变化趋势大体相同,贮藏温度越高,硫代巴比妥酸(thiobarbituric acid,TBA)值和挥发性盐基氮(total volatile basic nitrogen,TVB-N)含量越大,pH值越小;2℃恒温贮藏可以有效减少菌落总数,TVB-N含量、TBA值、pH值、质构变化缓慢,贮藏结束时,脆皮肠和方片火腿的菌落总数分别为3.24、2.94(lg(CFU/g)),TVB-N含量分别为57.64、66.53 mg/100 g,TBA值分别为0.15、0.14 mg/kg,pH值分别为5.98和5.91。8℃最不利于贮藏,分段贮藏组中2/8℃条件下贮藏样品的品质较好,恒定低温2℃或者前期低温贮藏更有利于保持产品品质稳定,延长肉制品货架期。
基金supported by the National Natural Science Foundation of China(Nos.22074003,22125401 and 22104127)the National Key R&D Program of China(No.2022YFC3400700).
文摘Exosomes,extracellular vesicles(EVs)that play crucial roles in biological processes,have emerged as attractive targets for noninvasive disease diagnosis and monitoring.Mass spectrometry(MS)offers high sensitivity,high throughput,and excellent qualification and quantification capacity,which is the ideal approach for exosome analysis.Nanomaterials’unique physiochemical properties,controllable morphology,and large surface area make them promising in biological sample pretreatment and detection.They contributed diverse functions to the exosome MS analysis,encompassing substrates for exosome isolation and cargos enrichment,matrices for laser desorption/ionization(LDI),read-out signals and supporters for signal amplification strategies,etc.In this review,recent progress in the applications of nanomaterial in the exosome MS analysis was summarized and a comprehensive discussion on the challenges and perspectives was proposed for the development of an advancing analysis approach of exosomes for the accurate diagnosis and monitoring of diseases.
