Cold-rolled thin strip steel of high flatness quality undergoes multistage deformation during tension leveling. Thus, the parameters of set-up and manipulating are more difficult. With the aid of FE code MSC. MARC, th...Cold-rolled thin strip steel of high flatness quality undergoes multistage deformation during tension leveling. Thus, the parameters of set-up and manipulating are more difficult. With the aid of FE code MSC. MARC, the tension leveling process of thin strip steel was numerically simulated. Concentrating on the influence of the roll intermeshes in 2# anti-cambering on the distribution and magnitude of residual stresses in leveled strip steel, several experiments were clone with the tension leveler based on the results from the simulation. It was found from the simulation that the magnitude of longitudinal residual stresses in the cross-section of the leveled strip steel regularly presents obvious interdependence with the roll intermeshes in 2# anti-cambering. In addition, there is a steady zone as the longitudinal residual stresses of the surface layers in leveled strip steel vary with the roll intermeshes of 2# anticambering, which is of importance in the manipulation of tension levelers. It was also found that the distribution of strains and stresses across the width of strip steel is uneven during leveling or after removing the tension loaded upon the strip, from which it was found that 3D simulation could not be replaced by 2D analysis because 2D analysis in this case cannot represent the physical behavior of strip steel deformation during tension leveling.展开更多
Background Nucleostemin is essential for the proliferation and survival of stem and cancer cells, but it is unknown whether this newly identified molecule is involved in prostate cancer pathogenesis.Methods Total RNA ...Background Nucleostemin is essential for the proliferation and survival of stem and cancer cells, but it is unknown whether this newly identified molecule is involved in prostate cancer pathogenesis.Methods Total RNA and protein were extracted from prostate cancer tissues and PC-3, LNCap and DU145 cell lines. The nucleostemin mRNA and protein expression were measured by RT-PCR and Western blot. Immunohistochemistry was also used to detect the nucleostemin protein expression in prostate cancer tissues and PC-3 cells. A nucleostemin specific, short hairpin RNA, expression plasmid was used to transfect PC-3 cells. The changes of nucleostemin gene were detected and the oroliferative capacitv of the cells was determined. Results Nucleostemin was highly expressed in prostate cancer tissues and cell lines. Nucleostemin expression level in the silencer group PC-3 cells remarkably reduced. The proliferation rate of silencer group PC-3 cells decreased and the percentage of G1 stage cells increased. The neoplasm forming capacity in nude mice of the silencer group PC-3 cells decreased significantly. Conclusions Nucleostemin is highly expressed in prostate cancer tissues and cell lines. The proliferative capacity of PC-3 cells is remarkably reduced after silencing nucleostemin gene expression.展开更多
基金Item Sponsored by Korea Research Foundation (KRF-2004-005-D00111)
文摘Cold-rolled thin strip steel of high flatness quality undergoes multistage deformation during tension leveling. Thus, the parameters of set-up and manipulating are more difficult. With the aid of FE code MSC. MARC, the tension leveling process of thin strip steel was numerically simulated. Concentrating on the influence of the roll intermeshes in 2# anti-cambering on the distribution and magnitude of residual stresses in leveled strip steel, several experiments were clone with the tension leveler based on the results from the simulation. It was found from the simulation that the magnitude of longitudinal residual stresses in the cross-section of the leveled strip steel regularly presents obvious interdependence with the roll intermeshes in 2# anti-cambering. In addition, there is a steady zone as the longitudinal residual stresses of the surface layers in leveled strip steel vary with the roll intermeshes of 2# anticambering, which is of importance in the manipulation of tension levelers. It was also found that the distribution of strains and stresses across the width of strip steel is uneven during leveling or after removing the tension loaded upon the strip, from which it was found that 3D simulation could not be replaced by 2D analysis because 2D analysis in this case cannot represent the physical behavior of strip steel deformation during tension leveling.
文摘Background Nucleostemin is essential for the proliferation and survival of stem and cancer cells, but it is unknown whether this newly identified molecule is involved in prostate cancer pathogenesis.Methods Total RNA and protein were extracted from prostate cancer tissues and PC-3, LNCap and DU145 cell lines. The nucleostemin mRNA and protein expression were measured by RT-PCR and Western blot. Immunohistochemistry was also used to detect the nucleostemin protein expression in prostate cancer tissues and PC-3 cells. A nucleostemin specific, short hairpin RNA, expression plasmid was used to transfect PC-3 cells. The changes of nucleostemin gene were detected and the oroliferative capacitv of the cells was determined. Results Nucleostemin was highly expressed in prostate cancer tissues and cell lines. Nucleostemin expression level in the silencer group PC-3 cells remarkably reduced. The proliferation rate of silencer group PC-3 cells decreased and the percentage of G1 stage cells increased. The neoplasm forming capacity in nude mice of the silencer group PC-3 cells decreased significantly. Conclusions Nucleostemin is highly expressed in prostate cancer tissues and cell lines. The proliferative capacity of PC-3 cells is remarkably reduced after silencing nucleostemin gene expression.
