Cell wall architecture plays a key role in stalk strength and forage digestibility.Lignin,cellulose,and hemicellulose are the three main components of plant cell walls,and they can impact stalk quality by affecting th...Cell wall architecture plays a key role in stalk strength and forage digestibility.Lignin,cellulose,and hemicellulose are the three main components of plant cell walls,and they can impact stalk quality by affecting the structure and strength of the cell wall.To explore cell wall development during secondary cell wall lignification in maize stalks,conventional and conditional genetic mapping were used to identify the dynamic quantitative trait loci(QTLs)of the cell wall components and digestibility traits during five growth stages after silking.Acid detergent lignin(ADL),cellulose(CEL),acid detergent fiber(ADF),neutral detergent fiber(NDF),and in vitro dry matter digestibility(IVDMD)were evaluated in a maize recombinant inbred line(RIL)population.ADL,CEL,ADF,and NDF gradually increased from 10 to 40 days after silking(DAS),and then they decreased.IVDMD initially decreased until 40 DAS,and then it increased slightly.Seventytwo QTLs were identified for the five traits,and each accounted for 3.48–24.04%of the phenotypic variation.Six QTL hotspots were found,and they were localized in the 1.08,2.04,2.07,7.03,8.05,and 9.03 bins of the maize genome.Within the interval of the pleiotropic QTL identified in bin 1.08 of the maize genome,six genes associated with cell wall component biosynthesis were identified as potential candidate genes for stalk strength as well as cell wall-related traits.In addition,26 conditional QTLs were detected in the five stages for all of the investigated traits.Twenty-two of the 26 conditional QTLs were found at 30 DAS conditioned using the values of 20 DAS,and at 50 DAS conditioned using the values of 40 DAS.These results indicated that cell wall-related traits are regulated by many genes,which are specifically expressed at different stages after silking.Simultaneous improvements in both forage digestibility and lodging resistance could be achieved by pyramiding multiple beneficial QTL alleles identified in this study.展开更多
目的研究快速康复外科(enhanced recovery after surgery,ERAS)方法在全髋关节置换术预防并发症的临床效果。方法选取河北医科大学第三医院行全髋关节置换术的患者作为研究对象,按照随机对照的原则分为研究组与对照组。研究组为实施ERA...目的研究快速康复外科(enhanced recovery after surgery,ERAS)方法在全髋关节置换术预防并发症的临床效果。方法选取河北医科大学第三医院行全髋关节置换术的患者作为研究对象,按照随机对照的原则分为研究组与对照组。研究组为实施ERAS护理方案组,对照组为常规护理组,评估并记录2组患者术后1、3、7 d及1个月的疼痛评分、术后相关并发症(深静脉血栓、泌尿系感染、坠积性肺炎、压疮、便秘)、Harris髋关节功能评分;并对住院时间、住院费用和患者满意度进行比较。结果研究组的VAS评分与对照组比较,2组的VAS评分随时间的延长而降低,2组在组间、时点间、组间·时点间交互作用方面差异有统计学意义(P<0.05),术后并发症发生率均低于对照组,Harris评分高于对照组,差异均有统计学意义(P<0.05)。研究组住院时间及住院平均费用均少于对照组,而患者满意度高于对照组,差异均有统计学意义(P<0.05)。结论ERAS应用于全髋关节置换术后的患者中,可减少术后并发症的发生,促进患者髋关节功能恢复,增加患者及家属的满意度。展开更多
【目的】Toll信号通路是昆虫中重要的免疫信号通路,其中Toll受体在保持Toll通路的正常免疫应答、抵抗外源病原体中起到关键的作用。本研究旨在探究肽聚糖和金黄色葡萄球菌Staphylococcus aureus对家蚕Bombyx mori Toll受体基因BmToll9-1...【目的】Toll信号通路是昆虫中重要的免疫信号通路,其中Toll受体在保持Toll通路的正常免疫应答、抵抗外源病原体中起到关键的作用。本研究旨在探究肽聚糖和金黄色葡萄球菌Staphylococcus aureus对家蚕Bombyx mori Toll受体基因BmToll9-1和BmToll9-2表达的影响。【方法】将革兰氏阳性菌细胞壁主要成分肽聚糖和革兰氏阳性菌金黄色葡萄球菌分别注射感染家蚕5龄第1天幼虫,诱导其发生免疫反应;采用实时荧光定量PCR分析注射后不同感染时间点BmToll9-2和BmToll9-1基因在家蚕幼虫中肠、表皮、脂肪体和丝腺中的相对表达水平。【结果】往家蚕5龄幼虫中注射肽聚糖或金黄色葡萄球菌后,BmToll9-2基因出现了时间和组织的差异性表达。注射肽聚糖和金黄色葡萄球菌均能诱导5龄幼虫中肠BmToll9-2基因的表达上调,注射肽聚糖和金黄色葡萄球菌分别在3和6 h时对基因表达的诱导效果最好,且注射金黄色葡萄球菌比注射肽聚糖对基因表达的诱导效果更好。注射金黄色葡萄球菌能引起5龄幼虫表皮、脂肪体和丝腺中BmToll9-2基因的表达上调,分别于注射后24, 6和24 h时诱导效果最好。注射金黄色葡萄球菌亦能诱导同源的BmToll9-1基因的上调表达。【结论】家蚕幼虫BmToll9基因在肽聚糖或金黄色葡萄球菌注射处理后均能在不同组织中发生上调表达,推测BmToll9基因参与了家蚕对肽聚糖和金黄色葡萄球菌的免疫反应。展开更多
650 nm AlGaInP/GaInP laser diodes with compressively strained MQW active layer have been successfully fabricated by means of single epitaxy growth.The threshold current is 6.4 mA,at 40 mA CW operation,the fundamental ...650 nm AlGaInP/GaInP laser diodes with compressively strained MQW active layer have been successfully fabricated by means of single epitaxy growth.The threshold current is 6.4 mA,at 40 mA CW operation,the fundamental transverse-mode still remains,and the output power and the slope efficiency can reach 34 mW and 1.1 mW/mA respectively.The dead output power in CW operation can reach 66 mW at a saturation current of 88 mA.During 200 h burn in test,the laser diodes show good stabilization with a degradation of less than 8%.展开更多
Clubiona theoblicki nom. nov.=Clubiona quadrata Yu &Li, 2019:161,f 7A-E,8A-H.(Primary homonym of Clubiona quadrata Kraus, 1955, now in Etymology. The species is named after Theo Blick, one of the editors of World ...Clubiona theoblicki nom. nov.=Clubiona quadrata Yu &Li, 2019:161,f 7A-E,8A-H.(Primary homonym of Clubiona quadrata Kraus, 1955, now in Etymology. The species is named after Theo Blick, one of the editors of World Spider Catalog, which has improved spider taxonomy worldwide;noun (name) in genitive case. Diagnosis and Description, see Yu & Li,2019.展开更多
基金the National Natural Science Foundation of China(31801367)the National Key Research and Development Program of China(2016YFD0101200)the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences.
文摘Cell wall architecture plays a key role in stalk strength and forage digestibility.Lignin,cellulose,and hemicellulose are the three main components of plant cell walls,and they can impact stalk quality by affecting the structure and strength of the cell wall.To explore cell wall development during secondary cell wall lignification in maize stalks,conventional and conditional genetic mapping were used to identify the dynamic quantitative trait loci(QTLs)of the cell wall components and digestibility traits during five growth stages after silking.Acid detergent lignin(ADL),cellulose(CEL),acid detergent fiber(ADF),neutral detergent fiber(NDF),and in vitro dry matter digestibility(IVDMD)were evaluated in a maize recombinant inbred line(RIL)population.ADL,CEL,ADF,and NDF gradually increased from 10 to 40 days after silking(DAS),and then they decreased.IVDMD initially decreased until 40 DAS,and then it increased slightly.Seventytwo QTLs were identified for the five traits,and each accounted for 3.48–24.04%of the phenotypic variation.Six QTL hotspots were found,and they were localized in the 1.08,2.04,2.07,7.03,8.05,and 9.03 bins of the maize genome.Within the interval of the pleiotropic QTL identified in bin 1.08 of the maize genome,six genes associated with cell wall component biosynthesis were identified as potential candidate genes for stalk strength as well as cell wall-related traits.In addition,26 conditional QTLs were detected in the five stages for all of the investigated traits.Twenty-two of the 26 conditional QTLs were found at 30 DAS conditioned using the values of 20 DAS,and at 50 DAS conditioned using the values of 40 DAS.These results indicated that cell wall-related traits are regulated by many genes,which are specifically expressed at different stages after silking.Simultaneous improvements in both forage digestibility and lodging resistance could be achieved by pyramiding multiple beneficial QTL alleles identified in this study.
文摘目的研究快速康复外科(enhanced recovery after surgery,ERAS)方法在全髋关节置换术预防并发症的临床效果。方法选取河北医科大学第三医院行全髋关节置换术的患者作为研究对象,按照随机对照的原则分为研究组与对照组。研究组为实施ERAS护理方案组,对照组为常规护理组,评估并记录2组患者术后1、3、7 d及1个月的疼痛评分、术后相关并发症(深静脉血栓、泌尿系感染、坠积性肺炎、压疮、便秘)、Harris髋关节功能评分;并对住院时间、住院费用和患者满意度进行比较。结果研究组的VAS评分与对照组比较,2组的VAS评分随时间的延长而降低,2组在组间、时点间、组间·时点间交互作用方面差异有统计学意义(P<0.05),术后并发症发生率均低于对照组,Harris评分高于对照组,差异均有统计学意义(P<0.05)。研究组住院时间及住院平均费用均少于对照组,而患者满意度高于对照组,差异均有统计学意义(P<0.05)。结论ERAS应用于全髋关节置换术后的患者中,可减少术后并发症的发生,促进患者髋关节功能恢复,增加患者及家属的满意度。
文摘650 nm AlGaInP/GaInP laser diodes with compressively strained MQW active layer have been successfully fabricated by means of single epitaxy growth.The threshold current is 6.4 mA,at 40 mA CW operation,the fundamental transverse-mode still remains,and the output power and the slope efficiency can reach 34 mW and 1.1 mW/mA respectively.The dead output power in CW operation can reach 66 mW at a saturation current of 88 mA.During 200 h burn in test,the laser diodes show good stabilization with a degradation of less than 8%.
文摘Clubiona theoblicki nom. nov.=Clubiona quadrata Yu &Li, 2019:161,f 7A-E,8A-H.(Primary homonym of Clubiona quadrata Kraus, 1955, now in Etymology. The species is named after Theo Blick, one of the editors of World Spider Catalog, which has improved spider taxonomy worldwide;noun (name) in genitive case. Diagnosis and Description, see Yu & Li,2019.
