Iridoschisis misdiagnosed as acute angle-closure glaucoma:a case report and literature review

We present the case of a patient with iridoschisis complicated with cataract,peripheral anterior synechiae(PAS),secondary glaucoma,and corneal endothelial damage.The patient was initially misdiagnosed with acute angle-closure glaucoma.Iridoschisis is a rare condition characterized by the splitting of the iris into two layers:the anterior layer breaks down into fibers,floating freely in the anterior chamber with a“shredded wheat”appearance.

TATA-box-binding protein-associated factor 15 is a novel biomarker that promotes cell proliferation and migration in gastrointestinal stromal tumor

BACKGROUND Gastrointestinal stromal tumor(GIST)is a common neoplasm with high rates of recurrence and metastasis,and its therapeutic efficacy is still not ideal.There is an unmet need to find new molecular therapeutic targets for GIST.TATA-boxbinding protein-associated factor 15(TAF15)contributes to the progress of various tumors,while the role and molecular mechanism of TAF15 in GIST progression are still unknown.AIM To explore new molecular therapeutic targets for GIST and understand the biological role and underlying mechanisms of TAF15 in GIST progression.METHODS Proteomic analysis was performed to explore the differentially expressed proteins in GIST.Western blotting and immunohistochemical analysis were used to verify the expression level of TAF15 in GIST tissues and cell lines.Cell counting kit-8,colony formation,wound-healing and transwell assay were executed to detect the ability of TAF15 on cell proliferation,migration and invasion.A xenograft mouse model was applied to explore the role of TAF15 in the progression of GIST.Western blotting was used to detect the phosphorylation level and total level of RAF1,MEK and ERK1/2.RESULTS A total of 1669 proteins were identified as differentially expressed proteins with 762 upregulated and 907 downregulated in GIST.TAF15 was selected for the further study because of its important role in cell proliferation and migration.TAF15 was significantly over expressed in GIST tissues and cell lines.Overexpression of TAF15 was associated with larger tumor size and higher risk stage of GIST.TAF15 knockdown significantly inhibited the cell proliferation and migration of GIST in vitro and suppressed tumor growth in vivo.Moreover,the inhibition of TAF15 expression significantly decreased the phosphorylation level of RAF1,MEK and ERK1/2 in GIST cells and xenograft tissues,while the total RAF1,MEK and ERK1/2 had no significant change.CONCLUSION TAF15 is over expressed in GIST tissues and cell lines.Overexpression of TAF15 was associated with a poor prognosis of GIST patients.TAF15 promotes cell proliferation and migration in GIST via the activation of the RAF1/MEK/ERK signaling pathway.Thus,TAF15 is expected to be a novel latent molecular biomarker or therapeutic target of GIST.

深部极松软围岩沿空巷道稳定性控制及应用

针对深部高应力作用下极松软围岩变形量大、破碎程度高、巷道难支护等问题,以丁集煤矿1232(3)沿空巷道为工程背景,采用数值模拟、现场监测和井下试验相结合的方法,对深部高应力极松软围岩沿空巷道破坏特征及阶段性控制原理和技术进行研究。结果表明:随着距巷道迎头距离的不断增大,巷道两帮应力的不对称性逐渐增大,实体煤侧应力峰值为37.18 MPa大于煤柱侧35.21 MPa,1242(3)终采线50 m范围内存在应力集中,最大达33 MPa,巷道围岩塑性区发育程度为煤柱帮大于实体煤帮大于顶底板;从巷道掘进过程中所经历的复杂围岩变化过程,将全巷道划分为5种典型的围岩变化阶段来分析围岩变形破坏特征和破坏机理,揭示了在高应力作用下深部软岩沿空巷道围岩变形量大,在空间上呈现出明显的区域性和非对称性的特征;基于巷道初步设计方案和围岩变形破坏特征,及时有效的调整支护方案,对巷道进行分段式、非对称、区域化综合治理,形成深部软岩巷道围岩控制长效机制,为同类型深部软岩巷道地压治理提供了理论和技术支撑。

Active components of Bupleuri Radix in the treatment of schizophrenia analyzed by network pharmacology and clinical verification

Background:Bupleuri Radix is a common Chinese medicinal material in traditional Chinese medicine.Currently,the therapeutic effect of treating schizophrenia is relatively well understood.However,there are fewer studies examining the underlying mechanisms of its treatment.The objective of the study was to investigate the primary mechanisms of Bupleuri Radix in treating schizophrenia through network pharmacology and clinical validation.Method:Network pharmacology revealed possible molecular mechanisms,followed by clinical verification.Sixty-seven schizophrenia patients undergoing treatment at the Hunan Brain Hospital between October and November 2022 were recruited and randomly divided into the olanzapine group and the olanzapine+Bupleuri Radix group.Additionally,32 healthy people undergoing physical examinations during the same period were included as the control group.The patient’s positive and negative symptom scale scores were compared.qPCR was used to detect the mRNA expression levels of ESR1,mTOR,EIF4E,and SMAD4 in peripheral blood.Results:Through network pharmacological analysis,it was concluded in this study that Bupleuri Radix might regulate the mTOR,PI3K-Akt,and HIF-1 signaling pathways.Clinical experiments indicated that compared with before treatment,the positive and negative symptom scale scores and total scores of the two treatment groups were significantly decreased after treatment(P<0.01).In addition,the positive and negative symptom scale scores and total scores in the olanzapine+Bupleuri Radix group were significantly decreased(P<0.01)compared to the olanzapine group after treatment.Before treatment,ESR1 mRNA expression levels in peripheral blood were significantly higher in the two treatment groups than in the control group,whereas the mRNA expression levels of mTOR,EIF4E,and SMAD4 in peripheral blood were significantly lower(P<0.01).The mRNA expression levels of mTOR,EIF4E,and SMAD4 in peripheral blood were significantly higher after therapy than before treatment,whereas the mRNA expression levels of ESR1 in peripheral blood were significantly lower(P<0.01).After therapy,the olanzapine+Bupleuri Radix group’s mRNA expression levels of mTOR,EIF4E,and SMAD4 were significantly higher than those of the olanzapine group,whereas the mRNA expression levels of ESR1 were significantly lower(P<0.01).Conclusion:The mechanism of Bupleuri Radix’s therapeutic efficacy in schizophrenia may involve the up-regulation of mTOR,EIF4E,and SMAD4 mRNA expression and the down-regulation of ESR1 mRNA expression in peripheral blood.

Comment on:Optic neuropathy and increased retinal glial fibrillary acidic protein due to microbead-induced ocular hypertension in the rabbit

Dear Editor,W e read with great interest the study by Zhao et al[1],which evaluated optic neuropathy and changes in the amount of glial fibrillary acidic protein in the retina with intracameral injection of microbeads into rabbits.The objective was to investigate whether the injection of microbeads into the anterior chamber can induce chronic intraocular hypertension in a glaucoma model in rabbits.

梨树腐烂病病原菌种群结构分析

【目的】分析新疆生产建设兵团第一师阿拉尔市库尔勒香梨园梨树腐烂病病原菌的种群分类,通过6对保守基因序列,研究库尔勒香梨腐烂病病原菌序列之间相关性及差异性。【方法】在库尔勒香梨园内采集具有典型症状的腐烂病病样,经科赫氏法则验证获取腐烂病病原菌纯培养。采用CTAB法提取腐烂病病原菌基因组DNA,通过PCR扩增ITS、β-tubulin、EF-1α、ACT、LSU、RBP2基因并测序,利用分子系统学分析不同基因片段的系统发育关系。【结果】在阿拉尔周边混交园内库尔勒香梨腐烂病菌种为Valsa mali var.pyri(V.ambiens、Valsa mali)。种内差异与地理位置有关,国内与国外梨腐烂病分离株差异较明显。EF-1α、ACT、LSU、RBP2在进化过程中更能够体现种间差异,系统发育树分支多,存在种间差异。【结论】新疆生产建设兵团第一师阿拉尔市库尔勒香梨腐烂病病原菌种群分布和种间差异性,库尔勒香梨园的腐烂病病原菌主要为Valsa mali var.pyri、Valsa sordida。新疆南疆绿洲特色果园种植模式,以杨树等为主防护林最为常见,存在着林果树木腐烂病交叉侵染的可能。

糖尿病与泌尿系结石关系的临床真实世界研究

目的分析真实世界中糖尿病与泌尿系结石的关系,为泌尿系结石的预防及治疗提供依据。方法收集2015年1月1日至2019年12月31日诊断有糖尿病、泌尿系结石及同期在体检中心常规体检人员的临床资料,分别设为糖尿病组、结石组、对照组(非糖尿病健康对照组和非结石健康对照组),记录各组的性别、年龄、是否有糖尿病、是否有血脂异常、是否有肾功能异常、是否有泌尿系结石等指标,倾向性评分匹配法匹配基线水平后,比较糖尿病组与非糖尿病健康对照组的结石发生率,结石组与非结石健康对照组的糖尿病发生率,探究临床真实世界中糖尿病与泌尿系结石的关系。结果经过校正组间性别、年龄、血脂水平、肾功能水平的差异后,糖尿病组患者的泌尿系结石发生率高于非糖尿病健康对照组(11.63%VS 5.42%),差异有统计学意义(χ^(2)=163.561,P<0.001);结石组患者的糖尿病发生率高于非结石健康对照组(15.70%VS 11.71%),差异有统计学意义(χ^(2)=40.171,P<0.001)。结论临床真实世界中糖尿病与泌尿系结石发病存在密切的关系,对糖尿病患者的早期有效干预可能有利于泌尿系结石的预防。

I型三电平储能变流器设计

随着新能源发电的快速发展,新能源发电输出功率的间歇性和不确定性问题日益突出,为增强电网对新能源的消纳能力,设计了1台I型三电平储能变流器。在深入分析其工作原理的基础上,构建了数学模型并给出了恒流充放电控制策略。针对三电平储能变流器中点电位不平衡的问题,提出了带可变分配因子的空间矢量脉冲宽度调制(SVPWM)算法以实现对中点电位波动的快速响应。样机实验结果表明,在恒流充放电控制策略下,样机在并网工况下具有良好的稳态和暂态性能,所采用的带可变分配因子的SVPWM算法能快速抑制中点电位波动且抑制效果明显。

“王荆公体”特征新论

王安石晚期作品特别是绝句,在艺术表现和审美风格上最为成熟,以其独特的艺术风貌被称为“王荆公体”。这些作品奠定了宋调的基本特征,又与苏、黄诗有着不同的风貌。当苏、黄等人正将宋调特征推向极致的时候,王诗晚期绝句却在呈现宋调特征的同时表现出向唐诗复归的倾向。“王荆公体”的独特风貌主要表现为精丽圆熟、生新工巧的语言特征,秀丽密集、轻灵明快的意象特征,清新淡远、空灵明净的意境特征,含蓄蕴藉、深婉曲折的风格特征。

基于无边界学习生涯的土木工程施工课程教学改革研究

随着科技的快速发展和全球化的推进,传统的土木工程施工课程教学模式已经难以满足当今社会的需求。为了培养出更多具备创新思维和实践能力的卓越工程师,总结了土木工程施工教学现状及存在问题,提出了学习方式灵活、专业跨界融合、创新导向、个性共性结合的“无边界学习生涯”理念。并探讨了在这种背景下,土木工程施工课程教学改革的方向和策略,旨在打破传统教学模式的限制,使学生能够更好地适应未来职业发展的需求。

临床医学专业网络教学模式开展初探

随着大数据时代的到来,以及各种突发事件对传统师教生学的教学模式的影响,线下的课堂教学的时空条件难以保证,线上教学势在必行。随着互联网和信息技术的飞速发展,以此为依托的远程网络教学逐渐得到广泛应用。为了构建全新的教学-评价模式,文章以临床医学专业为范例,拟构建一种创新型的网络教学-形成性评价体系。该体系充分利用丰富的网络资源,使用网络直播、翻转课堂等教学新技术、新方法,以现代化的网络信息技术在吸引学生注意力的同时,激发学生的学习兴趣,紧追时代潮流;以灵活监管与全程考核构建课程形成性评价体系,将全程考核替换传统的期末考试考核,强化知识重难点;以润物细无声的形式将考核任务分配到每次教学当中,深入探讨当下网络教学的可行性,以期促进教学相长的良性循环,使学生能够持续、自主学习。

Neoarchean granite-greenstone belts and related ore mineralization in the North China Craton:An overview

Tectonic processes involving amalgamations of microblocks along zones of ocean closure represented by granite-greenstone belts(GGB) were fundamental in building the Earth's early continents. The crustal growth and cratonization of the North China Craton(NCC) are correlated to the amalgamation of microblocks welded by 2.75-2.6 Ga and ~2.5 Ga GGBs. The lithological assemblages in the GGBs are broadly represented by volcano-sedimentary sequences, subduction-collision related granitoids and bimodal volcanic rocks(basalt and dacite) interlayered with minor komatiites and calc-alkalic volcanic rocks(basalt, andesite and felsic rock). The geochemical features of meta-basalts in the major GGBs of the NCC display affinity with N-MORB, E-MORB, OIB and calc-alkaline basalt, suggesting that the microblocks were separated by oceanic realm. The granitoid rocks display arc signature with enrichment of LILE(K,Rb, Sr, Ba) and LREE, and depletion of HFSE(Nb, Ta, Th, U, Ti) and HREE, and fall in the VAG field. The major mineralization includes Neoarchean BIF-type iron and VMS-type Cu-Zb deposits and these,together with the associated supracrustal rocks possibly formed in back-arc basins or arc-related oceanic slab subduction setting with or without input from mantle plumes. The 2.75-2.60 Ga TTG rocks,komatiites, meta-basalts and metasedimentary rocks in the Yanlingguan GGB are correlated to the upwelling mantle plume with eruption close to the continental margin within an ocean basin. The volcanosedimentary rocks and granitoid rocks in the late Neoarchean GGBs display formation ages of 2.60-2.48 Ga, followed by metamorphism at 2.52-2.47 Ga, corresponding to a typical modern-style subduction-collision system operating at the dawn of Proterozoic. The late Neoarchean komatiite(Dongwufenzi GGB), sanukitoid(Dongwufenzi GGB and Western Shandong GGB), BIF(Zunhua GGB) and VMS deposit(Hongtoushan-Qingyuan-Helong GGB) have closer connection to a combined process of oceanic slab subduction and mantle plume. The Neoarchean cratonization of the NCC appears to have involved two stages of tectonic process along the 2.75-2.6 Ga GGB and ~2.5 Ga GGBs, the former involve plume-arc interaction process, and the latter involving oceanic lithospheric subduction, with or without arcplume interaction.

某型船调距桨偶发螺距失控故障原因分析

针对某型船调距桨偶发螺距失控故障案例,基于调距桨电气控制、液压系统工作原理,采用故障树的分析方法,列举可能存在的故障原因序列,逐一排查分析,确定真实原因。

Construction of Biological Experiment Curriculum System in the Context of Innovation and Entrepreneurship Education

Innovation and entrepreneurship education is an important direction for the current reform of higher education and should be included in the professional talent training plan and the construction of the biological experiment curriculum system.Specific measures include in-depth excavation of the connotation of biological experiment teaching courses from the perspective of talent training,building an innovation and entrepreneurship biological experiment curriculum system guided by the needs of social development,establishing a comprehensive and diversified platform for practice and innovation,and constructing a teaching team that integrates innovation and entrepreneurship education and professional education.

Genesis of the Bianjiadayuan Pb-Zn polymetallic deposit, Inner Mongolia, China:Constraints from in-situ sulfur isotope and trace element geochemistry of pyrite

The Southern Great Xing’an Range (SGXR) which forms part of the eastern segment of the Central Asian Orogenic Belt (CAOB) is known as one of the most important Cu-Mo-Pb-Zn-Ag-Au metallogenic belts in China,hosting a number of porphyry Mo (Cu),skarn Fe (Sn),epithermal Au-Ag,and hydrothermal veintype Ag-Pb-Zn ore deposits.Here we investigate the Bianjiadayuan hydrothermal vein-type Ag-Pb-Zn ore deposit in the southern part of the SGXR.Porphyry Sn ±Cu ± Mo mineralization is also developed to the west of the Ag-Pb-Zn veins in the ore field.We identify a five-stage mineralization process based on field and petrologic studies including (i) the early porphyry mineralization stage,(ii) main porphyry mineralization stage,(iii) transition mineralization stage,(iv) vein-type mineralization stage and (v) late mineralization stage.Pyrite is the predominant sulfide mineral in all stages except in the late mineralization stage,and we identify corresponding four types of pyrites: Py1 is medium-grained subhedral to euhedral occurring in the early barren quartz vein;Py2 is medium- to fine-grained euhedral pyrite mainly coexisting with molybdenite,chalcopyrite,minor sphalerite and galena;Py3 is fine-grained,subhedral to irregular pyrite and displays cataclastic textures with micro-fractures;Py4 occurs as euhedral microcrystals and forms irregularly shaped aggregate with sphalerite and galena.LA-ICP-MS trace element analyses of pyrite show that Cu,Pb,Zn,Ag,Sn,Cd and Sb are partitioned into pyrite as structurally bound metals or mineral micro/nano-inclusions,whereas Co,Ni,As and Se enter the lattice via isomorphism in all types of pyrite.The Cu,Zn,Ag,Cd concentrations gradually increase from Py1 to Py4,which we correlate with cooling and mixing of ore-forming fluid with meteoric water.Py2 contains the highest contents of Co,Ni,Se,Te and Bi,suggesting high temperature conditions for the porphyry mineralization stage.Ratios of Co/Ni (0.03-10.79,average 2.13) and sulphur isotope composition of sulfide indicate typical hydrothermal origin for pyrites.The δ^34SCDT values of Py1 (0.42‰-1.61‰,average 1.16‰),Py2 (-1.23‰ to 0.82‰,average 0.35‰),Py3 (-0.36‰ to 2.47‰,average 0.97‰),Py4 (2.51‰-3.72‰,average 3.06‰),and other sulfides are consistent with those of typical porphyry deposit (-5‰ to 5‰),indicating that the Pb-Zn polymetallic mineralization in the Bianjiadayuan deposit is genetically linked to the Yanshanian (JurassiceCretaceous) magmatic-hydrothermal events.Variations of d34S values are ascribed to the changes in physical and chemical conditions during the evolution and migration of the ore-forming fluid.We propose that the high Sn content of pyrite in the Bianjiadayuan hydrothermal vein-type PbeZn polymetallic deposit can be used as a possible pathfinder to prospect for Sn mineralization in the surrounding area or deeper level of the ore field in this region.

Dry needling at myofascial trigger points mitigates chronic post-stroke shoulder spasticity

Post-stroke spasticity is associated with restriction in the range of motion of the shoulder.Reducing muscular dystrophy may help relieve muscular dysfunction in patients with post-stroke shoulder spasticity.Dry needle therapy is a method of needling the trigger points using a syringe needle without the use of a drug.Dry needle therapy is commonly used for pain at the shoulder,neck,waist,and back.In this case study,a 62-year-old male patient affected with cerebral hemorrhage of the right frontal lobe had received rehabilitative treatment for 12 years.However,he still experienced shoulder spasticity.The patient received daily dry needling at the trigger points of infraspinatus,teres minor,posterior deltoid,and pectoralis major on 9 days.After the first and ninth treatment,the Modified Ashworth Scale and the passive range of motion of the shoulder was used to assess the effect of the treatment.The spasticity and range of motion of the shoulder showed obvious improvement.These results indicate that dry needling at the myofascial trigger points can effectively treat chronic poststroke shoulder spasticity.

Neuroprotective effects of total saponins from Rubus parvifolius L. on cerebral ischemia/reperfusion injury in rats

This study examines the neuroprotective effects and mechanisms of action of total saponins from Rubus parvifolius L. （TSRP） on focal cerebral ischemia and reperfusion injury in rats. Focal cerebral ischemia and reperfusion injury was performed in rats using the suture method. The results indicate that intragastric injection of TSRP, at 5, 10 and 20 mg/kg, could decrease neurological impairment, reduce cerebral infarct volume, diminish pathological changes, and significantly inhibit the apoptosis of neurons surrounding the ischemic area. In addition, TSRP upregulated the expression of the anti-apoptotic factor Bcl-2, at the protein and mRNA levels, and it downregulated the expression of the pro-apoptotic factor Bax, at the protein and mRNA levels. These findings indicate that TSRP protects against cerebral ischemia/reperfusion injury, and that it may do so by regulating the expression of Bcl-2 and Bax.

天基为主的空间站测控通信模式

空间站时代为测控(TT&C)通信系统带来保障时间更长、覆盖要求更高、数据传输能力更强、航天员在轨工作生活体验更好等新的挑战,结合中继卫星系统(TDRSS)、卫星导航系统等天基测控通信手段的能力特点,提出以天基手段为主的解决方案,采用多星多舱段接力模式,将航天员出舱等重大活动的测控通信覆盖率提升至接近100%,采用多星多目标工作模式,较好地保障各种交会对接模式,综合利用中继卫星S频段多址(SMA)链路连续业务、短报文业务和北斗短报文业务常态化保障空间站安全运行,应用天地一体网络化技术,为航天员在轨上网、天地通信和载荷实验数据传输提供优质服务。

β-Ⅲ-Tubulin: a reliable marker for retinal ganglion cell labeling in experimental models of glaucoma

·AIM: To evaluate the reliability of β-III-Tubulin protein as a retinal ganglion cell(RGC) marker in the experimental glaucoma model.·METHODS: Glaucoma mouse models were established by injecting polystyrene microbeads into the anterior chamber of C57BL/6J mice, then their retinas were obtained 14 d and 28 d after the intraocular pressure(IOP)was elevated. Retinal flat mounts and sections were double-labeled by fluorogold(FG) and β-III-Tubulin antibody or single-labeled by β-III-Tubulin antibody,then RGCs were counted and compared respectively.· RESULTS: IOP of the injected eyes were elevated significantly and reached the peak at 22.8 ±0.7 mm Hg by day 14 after injection, then dropped to 11.3 ±0.7 mm Hg by day 28. RGC numbers counted by FG labeling and β-III- Tubulin antibody labeling were 64 807 ± 4930 and64 614 ±5054 respectively in the control group, with no significant difference. By day 14, RGCs in the experimental group decreased significantly compared to the control group, but there was no significant difference between the FG labeling counting and the β-III-Tubulin antibody labeling counting either in the experimental group or in the control group. The result was similar by day 28, with further RGC loss.·CONCLUSION: Our result suggested that the β-III-Tubulin protein was not affected by IOP elevation and can be used as a reliable marker for RGC in experimental models of glaucoma.