Using plants to produce heterologous proteins makes it very attractive due to the potentially low costs. Using this procedure it is possible to produce medicinal protein for clinical applications with the plants biore...Using plants to produce heterologous proteins makes it very attractive due to the potentially low costs. Using this procedure it is possible to produce medicinal protein for clinical applications with the plants bioreactors increasing gradually. The paper proposes the five major systems of the plant bioreactor as well as their advantage and disadvantage and the development of each system. Focuses on the five major systems of the plant bioreactor to produce vaccines, antibodies and medical protein and the research achievement at the present stage and the research on my laboratory. The key technology research of plant bioreactor such as new genes, new biological components, new technologies and new research methods related with plant bioreactor offer a work foundation for a long-term development in future.展开更多
目的:利用网络药理学和分子对接技术,探讨生血宁主要成分蚕沙治疗贫血的作用机制。方法:借助中药系统药理学数据库与分析平台(Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform,TCMSP)筛查生血宁有...目的:利用网络药理学和分子对接技术,探讨生血宁主要成分蚕沙治疗贫血的作用机制。方法:借助中药系统药理学数据库与分析平台(Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform,TCMSP)筛查生血宁有效成分,与GeneCards网站收集的靶点取交集后,筛选出生血宁治疗贫血的重合靶点,绘制中药-活性成分-靶点网络图。利用STRING构建药物治疗贫血的蛋白-蛋白相互作用网络(PPI),同时对重合靶点进行基因本体的分子功能和京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)通路富集分析,最后通过分子对接探究核心成分与核心靶点的相互作用。结果:基因本体(gene ontology,GO)分析和KEGG通路富集结果显示生血宁主要通过调节AMP激活的蛋白质激酶(AMP-activated protein kinase,AMPK)和丝裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK)等通路发挥治疗贫血作用。分子对接结果显示生血宁中活性成分可与蛋白激酶B1(又称AKT1)发生相互作用,其中叶酸和AKT1氨基酸残基ARG 379、ARG 369、RG 99和ASP 540等结合。结论:生血宁的作用机制涉及多靶点多通路,为阐明生血宁治疗贫血的药效学提供了理论依据。展开更多
基金National Ministry of Science and Technology "836" Project grant number:2007AA100503
文摘Using plants to produce heterologous proteins makes it very attractive due to the potentially low costs. Using this procedure it is possible to produce medicinal protein for clinical applications with the plants bioreactors increasing gradually. The paper proposes the five major systems of the plant bioreactor as well as their advantage and disadvantage and the development of each system. Focuses on the five major systems of the plant bioreactor to produce vaccines, antibodies and medical protein and the research achievement at the present stage and the research on my laboratory. The key technology research of plant bioreactor such as new genes, new biological components, new technologies and new research methods related with plant bioreactor offer a work foundation for a long-term development in future.
文摘目的:利用网络药理学和分子对接技术,探讨生血宁主要成分蚕沙治疗贫血的作用机制。方法:借助中药系统药理学数据库与分析平台(Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform,TCMSP)筛查生血宁有效成分,与GeneCards网站收集的靶点取交集后,筛选出生血宁治疗贫血的重合靶点,绘制中药-活性成分-靶点网络图。利用STRING构建药物治疗贫血的蛋白-蛋白相互作用网络(PPI),同时对重合靶点进行基因本体的分子功能和京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)通路富集分析,最后通过分子对接探究核心成分与核心靶点的相互作用。结果:基因本体(gene ontology,GO)分析和KEGG通路富集结果显示生血宁主要通过调节AMP激活的蛋白质激酶(AMP-activated protein kinase,AMPK)和丝裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK)等通路发挥治疗贫血作用。分子对接结果显示生血宁中活性成分可与蛋白激酶B1(又称AKT1)发生相互作用,其中叶酸和AKT1氨基酸残基ARG 379、ARG 369、RG 99和ASP 540等结合。结论:生血宁的作用机制涉及多靶点多通路,为阐明生血宁治疗贫血的药效学提供了理论依据。
