Chinese solar greenhouses enable the extension of the crop growing season in the cold climate in Northern China with little or no additional heating.The temporal variations of the air temperatures inside solar greenho...Chinese solar greenhouses enable the extension of the crop growing season in the cold climate in Northern China with little or no additional heating.The temporal variations of the air temperatures inside solar greenhouses located at three cities in North,Northeast and Northwest China were predicted by CFD simulations of the greenhouse systems using typical meteorological data.The predicted temperatures based on the meteorological data in Shenyang are quite similar to the measured temperatures.The results also show that the external air temperatures and solar radiation fluxes play more important roles for the inside temperatures as indicated by the highest inner temperature in the morning on Feb.18 and from 10:00 to 14:00 on Feb.19 in Beijing and by the predicted temperatures inside the greenhouse being higher in Lanzhou than those in Shenyang and Beijing during most of the day.The average daily temperature inside the greenhouse in Lanzhou was nearly 3.5℃higher than that in Shenyang.Predicted air temperatures for various wall designs show that for single walls,the daily average interior temperatures in the aerated concrete wall greenhouse were higher than those in the brick wall and reinforced concrete wall greenhouses.However,the air temperature fluctuations were lower in the reinforced concrete wall greenhouse due to greater thermal storage capacity.The results also show that the temperatures in the layered wall greenhouses are quite similar,which coincides with the experimental results。展开更多
目的 通过调查煤矿工人职业紧张与生命质量的情况,分析不同工种、工龄煤矿工人职业紧张与生命质量相关性。方法 采用整群抽样的方法,以班组为单位抽取2015年6月—2021年6月陕西省12家煤矿的810名煤矿工人为研究对象,调查煤矿工人基本情...目的 通过调查煤矿工人职业紧张与生命质量的情况,分析不同工种、工龄煤矿工人职业紧张与生命质量相关性。方法 采用整群抽样的方法,以班组为单位抽取2015年6月—2021年6月陕西省12家煤矿的810名煤矿工人为研究对象,调查煤矿工人基本情况,采用职业紧张量表(occupational stress inventory revised edition,OSI-R)、生命质量量表(36-item short form health survey,SF-36)评价煤矿工人职业紧张及生命质量情况。结果 井下工种(采煤、掘进、井下辅助工)职业紧张得分整体高于地面工种(地面筛分、地面辅助工)(均P<0.05)。井下工种中,掘进工得分最高;地面工种中,地面筛分工得分较高。不同工龄的煤矿工人职业任务特征、个体紧张反应和个体应变能力得分比较,差异均有统计学意义(均P<0.05),其中≥10年工龄组工人职业任务特征[(161.26±24.03)分]和个体紧张反应得分[(99.80±28.26)分]最高,1~<3年工龄工人个体应变能力得分最高[(129.06±20.78)分]。不同工种和工龄煤矿工人生理健康内容、心理健康内容和总体生命质量得分比较,差异均有统计学意义(均P<0.05),其中地面辅助工和1~<3年工龄组工人得分较高。职业任务特征、个体紧张反应及各子项与生理健康内容、心理健康内容和总体生命质量均呈负相关(均P<0.01),个体应变能力及各子项与生理健康内容、心理健康内容和总体生命质量均呈正相关(均P<0.01)。结论 井下作业会对煤矿工人职业紧张产生显著影响,并且随着工龄延长职业紧张水平增加;井下作业还会对煤矿工人生命质量产生显著影响。展开更多
This study aims to comprehensively study the effects of Ca^(2+) on the SA-induced resistance Botrytis cinerea in tomato through proteomics analysis.A proteomic approach was used to uncover the inducible proteins of to...This study aims to comprehensively study the effects of Ca^(2+) on the SA-induced resistance Botrytis cinerea in tomato through proteomics analysis.A proteomic approach was used to uncover the inducible proteins of tomato in the susceptible tomato cultivars ‘L402' against Botrytis cinerea after salicylic acid(SA) and a combination treatment of CaCl_2 and SA.The results showed that the use of combination treatment of CaCl_2 and SA significantly enhanced tomato resistance against Botrytis cinerea.In total,46 differentially expressed protein spots from 2-DE gel maps were detected,of which 41 were identified by mass spectrometry.All the identified proteins were categorized into eight groups according to their putative functions:defense response(14.00%),antioxidative protein(9.75%),photosynthesis(24.39%),molecular chaperone(4.88%),energy(17.01%),metabolism(21.95%),protein synthesis(4.88%) and signal transduction(0.2%).Of the proteins in the eight function groups,the effect of stress/defense and reactive oxygen species on Ca^(2+) -regulated SA-induced resistance may be the most important one in induced resistance by RT-PCR.The expression level of pathogenesis-related proteins(PRs) and chitinase was upregulated by a combination treatment of CaCl_2 and SA.The characterization of these proteins greatly helped to reveal the induced proteins involved in the regulation of Ca^(2+) on SA-induced resistance to Botrytis cinerea.In the combination treatment of CaCl_2 and SA,the defense response and antioxidative protein were clearly upregulated much more than SA alone or the control treatment by the method of proteomics and RT-PCR.The present findings suggest that susceptible tomato cultivars treated by the combination treatment of CaCl_2 and SA might possess a more sensitive SA signaling system or effective pathway than SA treatment alone.In addition,results indicated that SA could coordinate other cellular activities linked with photosynthesis and metabolism to facilitate defense response and recovery,indicating that the self-defense capability of tomato was improved by the combination treatment of CaCl_2 and SA.展开更多
基金the Ph.D.Programs Foundation of Liaoning Province,China(Grant No.20061040).
文摘Chinese solar greenhouses enable the extension of the crop growing season in the cold climate in Northern China with little or no additional heating.The temporal variations of the air temperatures inside solar greenhouses located at three cities in North,Northeast and Northwest China were predicted by CFD simulations of the greenhouse systems using typical meteorological data.The predicted temperatures based on the meteorological data in Shenyang are quite similar to the measured temperatures.The results also show that the external air temperatures and solar radiation fluxes play more important roles for the inside temperatures as indicated by the highest inner temperature in the morning on Feb.18 and from 10:00 to 14:00 on Feb.19 in Beijing and by the predicted temperatures inside the greenhouse being higher in Lanzhou than those in Shenyang and Beijing during most of the day.The average daily temperature inside the greenhouse in Lanzhou was nearly 3.5℃higher than that in Shenyang.Predicted air temperatures for various wall designs show that for single walls,the daily average interior temperatures in the aerated concrete wall greenhouse were higher than those in the brick wall and reinforced concrete wall greenhouses.However,the air temperature fluctuations were lower in the reinforced concrete wall greenhouse due to greater thermal storage capacity.The results also show that the temperatures in the layered wall greenhouses are quite similar,which coincides with the experimental results。
文摘目的 通过调查煤矿工人职业紧张与生命质量的情况,分析不同工种、工龄煤矿工人职业紧张与生命质量相关性。方法 采用整群抽样的方法,以班组为单位抽取2015年6月—2021年6月陕西省12家煤矿的810名煤矿工人为研究对象,调查煤矿工人基本情况,采用职业紧张量表(occupational stress inventory revised edition,OSI-R)、生命质量量表(36-item short form health survey,SF-36)评价煤矿工人职业紧张及生命质量情况。结果 井下工种(采煤、掘进、井下辅助工)职业紧张得分整体高于地面工种(地面筛分、地面辅助工)(均P<0.05)。井下工种中,掘进工得分最高;地面工种中,地面筛分工得分较高。不同工龄的煤矿工人职业任务特征、个体紧张反应和个体应变能力得分比较,差异均有统计学意义(均P<0.05),其中≥10年工龄组工人职业任务特征[(161.26±24.03)分]和个体紧张反应得分[(99.80±28.26)分]最高,1~<3年工龄工人个体应变能力得分最高[(129.06±20.78)分]。不同工种和工龄煤矿工人生理健康内容、心理健康内容和总体生命质量得分比较,差异均有统计学意义(均P<0.05),其中地面辅助工和1~<3年工龄组工人得分较高。职业任务特征、个体紧张反应及各子项与生理健康内容、心理健康内容和总体生命质量均呈负相关(均P<0.01),个体应变能力及各子项与生理健康内容、心理健康内容和总体生命质量均呈正相关(均P<0.01)。结论 井下作业会对煤矿工人职业紧张产生显著影响,并且随着工龄延长职业紧张水平增加;井下作业还会对煤矿工人生命质量产生显著影响。
基金supported by the National Natural Science Foundation of China under grants No.313084the National Higher-education Institution General Research and Development Project under Grant No.DC201502070403the Doctor Research Fund of Dalian Minzu University
文摘This study aims to comprehensively study the effects of Ca^(2+) on the SA-induced resistance Botrytis cinerea in tomato through proteomics analysis.A proteomic approach was used to uncover the inducible proteins of tomato in the susceptible tomato cultivars ‘L402' against Botrytis cinerea after salicylic acid(SA) and a combination treatment of CaCl_2 and SA.The results showed that the use of combination treatment of CaCl_2 and SA significantly enhanced tomato resistance against Botrytis cinerea.In total,46 differentially expressed protein spots from 2-DE gel maps were detected,of which 41 were identified by mass spectrometry.All the identified proteins were categorized into eight groups according to their putative functions:defense response(14.00%),antioxidative protein(9.75%),photosynthesis(24.39%),molecular chaperone(4.88%),energy(17.01%),metabolism(21.95%),protein synthesis(4.88%) and signal transduction(0.2%).Of the proteins in the eight function groups,the effect of stress/defense and reactive oxygen species on Ca^(2+) -regulated SA-induced resistance may be the most important one in induced resistance by RT-PCR.The expression level of pathogenesis-related proteins(PRs) and chitinase was upregulated by a combination treatment of CaCl_2 and SA.The characterization of these proteins greatly helped to reveal the induced proteins involved in the regulation of Ca^(2+) on SA-induced resistance to Botrytis cinerea.In the combination treatment of CaCl_2 and SA,the defense response and antioxidative protein were clearly upregulated much more than SA alone or the control treatment by the method of proteomics and RT-PCR.The present findings suggest that susceptible tomato cultivars treated by the combination treatment of CaCl_2 and SA might possess a more sensitive SA signaling system or effective pathway than SA treatment alone.In addition,results indicated that SA could coordinate other cellular activities linked with photosynthesis and metabolism to facilitate defense response and recovery,indicating that the self-defense capability of tomato was improved by the combination treatment of CaCl_2 and SA.
