Somatic embryogenesis ofKoelreuteria bipinnata var. integrifoliola was observed, plantlet formation in different types of somatic embryos was studied and the effect of abnormal embryos on plantlet formation was identi...Somatic embryogenesis ofKoelreuteria bipinnata var. integrifoliola was observed, plantlet formation in different types of somatic embryos was studied and the effect of abnormal embryos on plantlet formation was identified. Results show that somatic embryos of K. bipinnata var. integrifoliola include normal embryos, embryos with abnormal cotyledons, vitrified embryos, albino embryos, secondary embryos, linked embryos, embryos with abnormal growing points and embryos with expanding hypocotyl. After 40 d of callus culture, the response of normal somatic embryos from K. bipinnata var. integrifoliola was 26.7%, embryos with abnormal cotyledon 30.3% while other types of somatic embryos were below the 20% level. Most of normal embryos developed into plantlets and plantlet formation reached 94.9%. But the percentage of plantlet formation decreased apparently in abnormal embryos： the number of embryos with abnormal cotyledon declined to 76.1%, that of linked embryos to 47.4% and other types of abnormal embryos to below the 20% level. Albino embryos and embryos with abnormal growing point did not develop at all into plantlets. Embryos with abnormal cotyledons, linked embryos and embryos with abnormal growing points were observed during early stages of somatic embryogenesis, but vitrified, secondary and albino embryos and calli of embryos were observed at later stages. Increasing sucrose concentrations can decrease the occurrence of vitrified embryos, but the number of albino embryos decreased with an in- creasing in sucrose concentration.展开更多
目的探讨超声引导下乳腺微创旋切术治疗良性乳腺肿块的临床效果。方法62例良性乳腺肿块患者,依照随机数字表法分为对照组和观察组,各31例。对照组患者采用传统开放手术治疗,观察组患者采用超声引导下乳腺微创旋切术治疗。比较两组手术...目的探讨超声引导下乳腺微创旋切术治疗良性乳腺肿块的临床效果。方法62例良性乳腺肿块患者,依照随机数字表法分为对照组和观察组,各31例。对照组患者采用传统开放手术治疗,观察组患者采用超声引导下乳腺微创旋切术治疗。比较两组手术指标、术后并发症发生情况、视觉模拟评分法(VAS)评分、感觉神经功能恢复时间、乳房外观评分。结果观察组切口长度(4.18±1.20)mm、切口愈合时间(3.52±1.16)d、手术时间(17.52±4.20)min均短于对照组的(27.56±3.24)mm、(6.65±1.52)d、(30.15±6.28)min,术中出血量(14.26±1.75)ml少于对照组的(21.56±3.20)ml,差异有统计学意义(P<0.05)。观察组术后并发症发生率3.23%明显低于对照组的19.35%,差异有统计学意义(P<0.05)。观察组术后6、12、24 h VAS评分分别为(4.63±1.20)、(3.52±0.85)、(2.18±0.66)分,均低于对照组的(5.96±1.23)、(5.23±0.98)、(3.78±0.88)分,差异有统计学意义(P<0.05)。观察组触觉恢复时间(146.28±15.20)min和痛觉恢复时间(194.23±15.74)min均短于对照组的(156.28±16.80)、(203.33±16.55)min,差异有统计学意义(P<0.05)。观察组乳头乳晕、切口瘢痕、色素沉着、乳房形状评分分别为(91.23±3.25)、(92.20±3.15)、(91.47±3.58)、(91.66±3.45)分,均高于对照组的(84.26±3.55)、(85.39±3.47)、(84.20±3.45)、(85.46±3.49)分,差异有统计学意义(P<0.05)。结论对良性乳腺肿块患者采取超声引导下乳腺微创旋切术治疗的疗效显著,且安全性高,术后疼痛感较轻微,创伤小,值得采纳。展开更多
X射线源是X射线显微成像系统的核心部件,其功率密度和焦斑大小与聚焦系统密切相关。本文对高功率密度微焦斑X射线源的聚焦系统进行了优化设计,包括透镜结构设计、多透镜工作模式的选择、透镜间距优化等。本文设计了小球差系数的物镜;提...X射线源是X射线显微成像系统的核心部件,其功率密度和焦斑大小与聚焦系统密切相关。本文对高功率密度微焦斑X射线源的聚焦系统进行了优化设计,包括透镜结构设计、多透镜工作模式的选择、透镜间距优化等。本文设计了小球差系数的物镜;提出了由聚光镜、辅助聚光镜和物镜组成的多透镜聚焦系统,以实现系统较大的缩放比率和束流较高的传递效率;优化了透镜间距,以保证在满足系统缩放比率的前提下减小聚光镜的安匝数。最后,运用MEBS公司专业电子束计算软件,计算仿真了160 k V的加速电压下聚焦系统中透镜的场分布、电子的运动轨迹、像差、束斑分布等电子光学参量,最终得到束流为80μA,束斑为0. 9μm,功率密度优于1 W/μm^2的微束斑电子束。本文设计的聚焦系统为高功率密度微焦斑X射线源的设计和研制提供了依据。展开更多
基金supported by the Foundation for Science and Technology Research and Development Program of Hebei Province (09225511)the Project of the Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of the Ministry of Education, Beijing Forestry University, China (05– 04)
文摘Somatic embryogenesis ofKoelreuteria bipinnata var. integrifoliola was observed, plantlet formation in different types of somatic embryos was studied and the effect of abnormal embryos on plantlet formation was identified. Results show that somatic embryos of K. bipinnata var. integrifoliola include normal embryos, embryos with abnormal cotyledons, vitrified embryos, albino embryos, secondary embryos, linked embryos, embryos with abnormal growing points and embryos with expanding hypocotyl. After 40 d of callus culture, the response of normal somatic embryos from K. bipinnata var. integrifoliola was 26.7%, embryos with abnormal cotyledon 30.3% while other types of somatic embryos were below the 20% level. Most of normal embryos developed into plantlets and plantlet formation reached 94.9%. But the percentage of plantlet formation decreased apparently in abnormal embryos： the number of embryos with abnormal cotyledon declined to 76.1%, that of linked embryos to 47.4% and other types of abnormal embryos to below the 20% level. Albino embryos and embryos with abnormal growing point did not develop at all into plantlets. Embryos with abnormal cotyledons, linked embryos and embryos with abnormal growing points were observed during early stages of somatic embryogenesis, but vitrified, secondary and albino embryos and calli of embryos were observed at later stages. Increasing sucrose concentrations can decrease the occurrence of vitrified embryos, but the number of albino embryos decreased with an in- creasing in sucrose concentration.
文摘目的探讨超声引导下乳腺微创旋切术治疗良性乳腺肿块的临床效果。方法62例良性乳腺肿块患者,依照随机数字表法分为对照组和观察组,各31例。对照组患者采用传统开放手术治疗,观察组患者采用超声引导下乳腺微创旋切术治疗。比较两组手术指标、术后并发症发生情况、视觉模拟评分法(VAS)评分、感觉神经功能恢复时间、乳房外观评分。结果观察组切口长度(4.18±1.20)mm、切口愈合时间(3.52±1.16)d、手术时间(17.52±4.20)min均短于对照组的(27.56±3.24)mm、(6.65±1.52)d、(30.15±6.28)min,术中出血量(14.26±1.75)ml少于对照组的(21.56±3.20)ml,差异有统计学意义(P<0.05)。观察组术后并发症发生率3.23%明显低于对照组的19.35%,差异有统计学意义(P<0.05)。观察组术后6、12、24 h VAS评分分别为(4.63±1.20)、(3.52±0.85)、(2.18±0.66)分,均低于对照组的(5.96±1.23)、(5.23±0.98)、(3.78±0.88)分,差异有统计学意义(P<0.05)。观察组触觉恢复时间(146.28±15.20)min和痛觉恢复时间(194.23±15.74)min均短于对照组的(156.28±16.80)、(203.33±16.55)min,差异有统计学意义(P<0.05)。观察组乳头乳晕、切口瘢痕、色素沉着、乳房形状评分分别为(91.23±3.25)、(92.20±3.15)、(91.47±3.58)、(91.66±3.45)分,均高于对照组的(84.26±3.55)、(85.39±3.47)、(84.20±3.45)、(85.46±3.49)分,差异有统计学意义(P<0.05)。结论对良性乳腺肿块患者采取超声引导下乳腺微创旋切术治疗的疗效显著,且安全性高,术后疼痛感较轻微,创伤小,值得采纳。
文摘X射线源是X射线显微成像系统的核心部件,其功率密度和焦斑大小与聚焦系统密切相关。本文对高功率密度微焦斑X射线源的聚焦系统进行了优化设计,包括透镜结构设计、多透镜工作模式的选择、透镜间距优化等。本文设计了小球差系数的物镜;提出了由聚光镜、辅助聚光镜和物镜组成的多透镜聚焦系统,以实现系统较大的缩放比率和束流较高的传递效率;优化了透镜间距,以保证在满足系统缩放比率的前提下减小聚光镜的安匝数。最后,运用MEBS公司专业电子束计算软件,计算仿真了160 k V的加速电压下聚焦系统中透镜的场分布、电子的运动轨迹、像差、束斑分布等电子光学参量,最终得到束流为80μA,束斑为0. 9μm,功率密度优于1 W/μm^2的微束斑电子束。本文设计的聚焦系统为高功率密度微焦斑X射线源的设计和研制提供了依据。
