Objective To study the role of insulin-like growth factor II receptor in free silica-induced transdifferentiation of primary rat lung fibroblasts Methods Rat lung fibroblasts and rat alveolar macrophages were cultured...Objective To study the role of insulin-like growth factor II receptor in free silica-induced transdifferentiation of primary rat lung fibroblasts Methods Rat lung fibroblasts and rat alveolar macrophages were cultured. A transdifferentiation model of primary rat lung fibroblasts was induced by free silica. Levels of a-SMA protein, IGF-liR protein and mRNA were measured by immunocytochemistry, Western blot and RT-PCR, respectively. Lung fibroblasts were treated with Wortmannin. Results The expression levels of a-SMA concentration and decreased after Wortmann and IGF-IIR increased with the increasing free silica n was used. Conclusion The IGF-IIR plays an important role in free silica-induced transdifferentiation of primary rat lung fibroblasts.展开更多
Objective To analyze the expression of different proteins in free silica-induced transdifferentiated rat lung fibrob^asts. Methods Rat lung fibroblasts and alveolar macrophages were cultured. A transdifferentiation mo...Objective To analyze the expression of different proteins in free silica-induced transdifferentiated rat lung fibrob^asts. Methods Rat lung fibroblasts and alveolar macrophages were cultured. A transdifferentiation model of rat lung fibroblasts was established. Free silica was used as a stimulator for rat lung fibroblasts. Changes in a-SMA were detected by immunohistochemistry and Western blot, respectively. Protein of lung fibroblasts was extracted and analyzed by two-dimensional electrophoresis （2-DE）. Results Six protein spots were identified by mass spectrometry, including glyceraldehyde 3-phosphate-dehydrogenase, peroxiredoxin 5, heterogeneous nuclear ribonucleoprotein A2, transgelin 2, keratin K6 and vimentin. Conclusion Some proteins are changed in free silica-induced transdifferentiaed rat lung fibroblasts展开更多
BcpCAL, the homologous gene of CAL, was isolated from Chinese cabbage. Unlike bobcat of cauliflower, BcpCAL did not hold the terminating mutation in the fifth e-con. After crosses of cauliflower with Chinese cabbage, ...BcpCAL, the homologous gene of CAL, was isolated from Chinese cabbage. Unlike bobcat of cauliflower, BcpCAL did not hold the terminating mutation in the fifth e-con. After crosses of cauliflower with Chinese cabbage, the resultant hybrids failed to form curd, which implicates the genetic complement of BcpCAL to the mutated Bob- CAL in the function of curd formation. One of CAL gene isolated from the hybrid apparently comes from the female parent (Chinese cabbage) even though there are a few of the biases substituted and deleted. The result offers the molecular and genetic evidences for the Study of biological function of CAL in morphological genetics of curd.展开更多
基金supported by the Research Fund from theNational Natural Science Foundation of China(#81102109)
文摘Objective To study the role of insulin-like growth factor II receptor in free silica-induced transdifferentiation of primary rat lung fibroblasts Methods Rat lung fibroblasts and rat alveolar macrophages were cultured. A transdifferentiation model of primary rat lung fibroblasts was induced by free silica. Levels of a-SMA protein, IGF-liR protein and mRNA were measured by immunocytochemistry, Western blot and RT-PCR, respectively. Lung fibroblasts were treated with Wortmannin. Results The expression levels of a-SMA concentration and decreased after Wortmann and IGF-IIR increased with the increasing free silica n was used. Conclusion The IGF-IIR plays an important role in free silica-induced transdifferentiation of primary rat lung fibroblasts.
基金supported by the National Natural Science Foundation of China(#81102109)the Basic Research and Frontier Technology Projects of Henan Province(#72300450190)
文摘Objective To analyze the expression of different proteins in free silica-induced transdifferentiated rat lung fibrob^asts. Methods Rat lung fibroblasts and alveolar macrophages were cultured. A transdifferentiation model of rat lung fibroblasts was established. Free silica was used as a stimulator for rat lung fibroblasts. Changes in a-SMA were detected by immunohistochemistry and Western blot, respectively. Protein of lung fibroblasts was extracted and analyzed by two-dimensional electrophoresis （2-DE）. Results Six protein spots were identified by mass spectrometry, including glyceraldehyde 3-phosphate-dehydrogenase, peroxiredoxin 5, heterogeneous nuclear ribonucleoprotein A2, transgelin 2, keratin K6 and vimentin. Conclusion Some proteins are changed in free silica-induced transdifferentiaed rat lung fibroblasts
文摘BcpCAL, the homologous gene of CAL, was isolated from Chinese cabbage. Unlike bobcat of cauliflower, BcpCAL did not hold the terminating mutation in the fifth e-con. After crosses of cauliflower with Chinese cabbage, the resultant hybrids failed to form curd, which implicates the genetic complement of BcpCAL to the mutated Bob- CAL in the function of curd formation. One of CAL gene isolated from the hybrid apparently comes from the female parent (Chinese cabbage) even though there are a few of the biases substituted and deleted. The result offers the molecular and genetic evidences for the Study of biological function of CAL in morphological genetics of curd.
