This paper studies the extraction and identification of ginsenoside Re from ginseng fruits and investigates the effect and mechanism of ginsenoside Re of protecting acute renal ischemia-reperfusion injury in rats.Havi...This paper studies the extraction and identification of ginsenoside Re from ginseng fruits and investigates the effect and mechanism of ginsenoside Re of protecting acute renal ischemia-reperfusion injury in rats.Having been smashed,the ginseng fruits were ultrasonically extracted twice with 95% ethanol for 30min each.Having been concentrated,the solution was dissolved with distilled water and separated by two-column chromatography,of which one was packed with macroporous resin D4020,and the other was packed with macroporous resin D941.The raw product was dissolved with methanol and was purified by elution on a Si gel column,finally ginsenoside Re was obtained.The structure of the ginsenoside Re was analyzed by the thin-layer chromatography and NMR methods,and HPLC was carried for the content determination.The model of acute renal ischemia-reperfusion injury in rats was established after ischemia for 1 h and reperfusion for 1 h or 24 h,serum SOD(superoxide dismutase),MDA(malondialdehyde) and plasma TXB2(thromboxane B2) and 6-keto-PGF1α were detected.The results show that it has accurately,fast,convenient merits and so on.Ginsenoside Re has a protective effect on acute renal ischemia-reperfusion injury in rats,the mechanism may be related to improving the imbalance of thromboxane A2(TXA2)/prostacyclin(PGI2) and inhibiting lipid peroxidation reaction.展开更多
目的 观察产后盆底肌修复中采用盆底肌肉锻炼结合电刺激与生物反馈的效果及对国际尿失禁问卷调查简表(ICIQ-UI Short Form, ICIQ-SF)和女性性功能指数量表(Female Sexual Function Index, FSFI)评分的影响。方法 选取2022年5月—2023年1...目的 观察产后盆底肌修复中采用盆底肌肉锻炼结合电刺激与生物反馈的效果及对国际尿失禁问卷调查简表(ICIQ-UI Short Form, ICIQ-SF)和女性性功能指数量表(Female Sexual Function Index, FSFI)评分的影响。方法 选取2022年5月—2023年10月期间至郑州大学第一附属医院产科展开盆底肌修复的120例患者纳入研究,按照不同的干预方式将患者分为研究组和常规组,每组各60例患者。常规组采用盆底肌肉锻炼+生物反馈治疗,研究组则在此基础之上结合电刺激治疗,对比两组患者干预效果。结果 研究组盆底肌力恢复率、子宫脱垂消失率以及尿失禁改善率分别为100.00%、96.67%及93.33%,明显优于常规组(P<0.05);两组患者在治疗之前的最大快肌收缩值、60s和10s慢肌收缩比较无统计学意义(P>0.05);研究组治疗后最大快肌收缩值、60s和10s慢肌收缩指标分别为(38.69±7.79)μV、(26.37±6.64)μV和(32.17±4.28)μV,显著高于常规组(P<0.05);治疗前两组患者ICIQ-SF各项评分比较无统计学意义(P>0.05),研究组治疗后ICIQ-SF尿失禁对生活的影响、漏尿频率、漏尿量及总分均低于常规组(P<0.05);治疗前两组患者FSFI各项评分比较无意义(P>0.05),研究组FSFI性生活的满意度、性高潮、阴道的润滑度、性唤起和性欲评分明显高于常规组,性交疼痛评分低于常规组(P<0.05)。结论 盆底功能障碍采用盆底肌肉锻炼结合生物反馈与电刺激,不仅可以对患者肌纤维进行有效改善,同时还可以促使患者子宫脱垂以及漏尿等不良症状进行缓解,对于性生活质量的提升有显著的效果,值得进行推广。展开更多
基金Supported by the National Natural Science Foundation of China(No.30701128)the Fund of Jilin Provincial Science and Technology Department,China(No.200705377)
文摘This paper studies the extraction and identification of ginsenoside Re from ginseng fruits and investigates the effect and mechanism of ginsenoside Re of protecting acute renal ischemia-reperfusion injury in rats.Having been smashed,the ginseng fruits were ultrasonically extracted twice with 95% ethanol for 30min each.Having been concentrated,the solution was dissolved with distilled water and separated by two-column chromatography,of which one was packed with macroporous resin D4020,and the other was packed with macroporous resin D941.The raw product was dissolved with methanol and was purified by elution on a Si gel column,finally ginsenoside Re was obtained.The structure of the ginsenoside Re was analyzed by the thin-layer chromatography and NMR methods,and HPLC was carried for the content determination.The model of acute renal ischemia-reperfusion injury in rats was established after ischemia for 1 h and reperfusion for 1 h or 24 h,serum SOD(superoxide dismutase),MDA(malondialdehyde) and plasma TXB2(thromboxane B2) and 6-keto-PGF1α were detected.The results show that it has accurately,fast,convenient merits and so on.Ginsenoside Re has a protective effect on acute renal ischemia-reperfusion injury in rats,the mechanism may be related to improving the imbalance of thromboxane A2(TXA2)/prostacyclin(PGI2) and inhibiting lipid peroxidation reaction.
文摘目的 观察产后盆底肌修复中采用盆底肌肉锻炼结合电刺激与生物反馈的效果及对国际尿失禁问卷调查简表(ICIQ-UI Short Form, ICIQ-SF)和女性性功能指数量表(Female Sexual Function Index, FSFI)评分的影响。方法 选取2022年5月—2023年10月期间至郑州大学第一附属医院产科展开盆底肌修复的120例患者纳入研究,按照不同的干预方式将患者分为研究组和常规组,每组各60例患者。常规组采用盆底肌肉锻炼+生物反馈治疗,研究组则在此基础之上结合电刺激治疗,对比两组患者干预效果。结果 研究组盆底肌力恢复率、子宫脱垂消失率以及尿失禁改善率分别为100.00%、96.67%及93.33%,明显优于常规组(P<0.05);两组患者在治疗之前的最大快肌收缩值、60s和10s慢肌收缩比较无统计学意义(P>0.05);研究组治疗后最大快肌收缩值、60s和10s慢肌收缩指标分别为(38.69±7.79)μV、(26.37±6.64)μV和(32.17±4.28)μV,显著高于常规组(P<0.05);治疗前两组患者ICIQ-SF各项评分比较无统计学意义(P>0.05),研究组治疗后ICIQ-SF尿失禁对生活的影响、漏尿频率、漏尿量及总分均低于常规组(P<0.05);治疗前两组患者FSFI各项评分比较无意义(P>0.05),研究组FSFI性生活的满意度、性高潮、阴道的润滑度、性唤起和性欲评分明显高于常规组,性交疼痛评分低于常规组(P<0.05)。结论 盆底功能障碍采用盆底肌肉锻炼结合生物反馈与电刺激,不仅可以对患者肌纤维进行有效改善,同时还可以促使患者子宫脱垂以及漏尿等不良症状进行缓解,对于性生活质量的提升有显著的效果,值得进行推广。