详细介绍了Philips Allura Xper FD20平板血管造影系统使用过程中出现的机架控制模块无法实现机架的运动、机器开机后无法进入应用界面和系统曝光透视时屏幕无图像显示、曝光透视过程中无射线等故障维修过程,为相关维修人员维修类似设...详细介绍了Philips Allura Xper FD20平板血管造影系统使用过程中出现的机架控制模块无法实现机架的运动、机器开机后无法进入应用界面和系统曝光透视时屏幕无图像显示、曝光透视过程中无射线等故障维修过程,为相关维修人员维修类似设备故障提供参考。指出了只有做好日常维护保养工作,才能降低设备的故障发生率,保证其正常运行。展开更多
A specialized test of two-hybrid library type three-frame cDNA yeast for Muskmelon Fusarium oxysporum using the switching mechanism at the 5'end of RNA template(SMART)technology was constructed to screen for inter...A specialized test of two-hybrid library type three-frame cDNA yeast for Muskmelon Fusarium oxysporum using the switching mechanism at the 5'end of RNA template(SMART)technology was constructed to screen for interaction protein genes for wilt disease and to further research the molecular mechanisms of Fusarium oxysporum pathogenesis to explain the interactions between plant and pathogen.A 500-bp cDNA was purified and extracted using SMART and LD-PCR technology to synthesize ds cDNA and was then homogenized and purified to remove the fragments.After processing,the ds cDNA was connected to three types of reading frame pGADT7-SfiI carriers,and the three connection products in E.coli Electrocell were used to build the primary cDNA library.The titer of three ORF cDNA primary library storage capacities was 2.6×10^6,1.8×10^6 and 3×10^6 cfu;the PCR identification of the ORF 1 and 2 gene recombination rate was 94%,the ORF 3 gene recombination rate was 100%,and the insert length distribution was 0.5-4.0 kb as a single band.To reach the quality requirements for library construction,three kinds of reading frame cDNA primary libraries were mixed and amplified,and the plasmid was transformed into the Y187 yeast strain.The titer of the Y187 yeast library was determined to be 3.5×107 cfu?mL-1,and the base of the yeast library was approximately 1 600 000 cfu.The results showed that the construction of muskmelon Fusarium-specific two-hybrid library type three-frame cDNA yeast had a higher reservoir capacity and recombination rate and met the yeast two-hybrid screening requirements.展开更多
铁氧体隔离器作为一种大量应用于5G通讯基站的通讯元器件,其可靠性关系到基站整机运行的持续性与稳定性,而隔离器的衰减片存在的焊接空洞问题可能导致隔离器在再流焊过程中发生失效,严重时导致基站电路板烧毁,因此管控及降低衰减片焊接...铁氧体隔离器作为一种大量应用于5G通讯基站的通讯元器件,其可靠性关系到基站整机运行的持续性与稳定性,而隔离器的衰减片存在的焊接空洞问题可能导致隔离器在再流焊过程中发生失效,严重时导致基站电路板烧毁,因此管控及降低衰减片焊接空洞率十分重要。首先对铁氧体隔离器的衰减片焊接空洞产生的机理进行分析,并通过实验设计(Design of Experiment,DOE)对点锡过程中可能导致焊接空洞问题的因素进行了析因分析及点锡工艺的优化。实验结果表明,通过控制点锡轨迹及点锡速度,能显著减少焊接空洞,并明显提升该焊接过程的制程能力,大幅提升了分布参数隔离器的可靠度,降低了隔离器经历再流焊后失效的风险。展开更多
文摘详细介绍了Philips Allura Xper FD20平板血管造影系统使用过程中出现的机架控制模块无法实现机架的运动、机器开机后无法进入应用界面和系统曝光透视时屏幕无图像显示、曝光透视过程中无射线等故障维修过程,为相关维修人员维修类似设备故障提供参考。指出了只有做好日常维护保养工作,才能降低设备的故障发生率,保证其正常运行。
基金Supported by the National Nature Science Foundation of China(31372088)the "Academic Backbone" Project of Northeast Agricultural University(15XG05)China Agriculture Research System(CARS-26-02)
文摘A specialized test of two-hybrid library type three-frame cDNA yeast for Muskmelon Fusarium oxysporum using the switching mechanism at the 5'end of RNA template(SMART)technology was constructed to screen for interaction protein genes for wilt disease and to further research the molecular mechanisms of Fusarium oxysporum pathogenesis to explain the interactions between plant and pathogen.A 500-bp cDNA was purified and extracted using SMART and LD-PCR technology to synthesize ds cDNA and was then homogenized and purified to remove the fragments.After processing,the ds cDNA was connected to three types of reading frame pGADT7-SfiI carriers,and the three connection products in E.coli Electrocell were used to build the primary cDNA library.The titer of three ORF cDNA primary library storage capacities was 2.6×10^6,1.8×10^6 and 3×10^6 cfu;the PCR identification of the ORF 1 and 2 gene recombination rate was 94%,the ORF 3 gene recombination rate was 100%,and the insert length distribution was 0.5-4.0 kb as a single band.To reach the quality requirements for library construction,three kinds of reading frame cDNA primary libraries were mixed and amplified,and the plasmid was transformed into the Y187 yeast strain.The titer of the Y187 yeast library was determined to be 3.5×107 cfu?mL-1,and the base of the yeast library was approximately 1 600 000 cfu.The results showed that the construction of muskmelon Fusarium-specific two-hybrid library type three-frame cDNA yeast had a higher reservoir capacity and recombination rate and met the yeast two-hybrid screening requirements.
文摘铁氧体隔离器作为一种大量应用于5G通讯基站的通讯元器件,其可靠性关系到基站整机运行的持续性与稳定性,而隔离器的衰减片存在的焊接空洞问题可能导致隔离器在再流焊过程中发生失效,严重时导致基站电路板烧毁,因此管控及降低衰减片焊接空洞率十分重要。首先对铁氧体隔离器的衰减片焊接空洞产生的机理进行分析,并通过实验设计(Design of Experiment,DOE)对点锡过程中可能导致焊接空洞问题的因素进行了析因分析及点锡工艺的优化。实验结果表明,通过控制点锡轨迹及点锡速度,能显著减少焊接空洞,并明显提升该焊接过程的制程能力,大幅提升了分布参数隔离器的可靠度,降低了隔离器经历再流焊后失效的风险。
