[Objective] The aim was to improve the saccharification of wheat bran cellulose. [Method] Taking the wheat bran as raw materials, and using orthogonal method, the effects of acid concentration, temperature, times and ...[Objective] The aim was to improve the saccharification of wheat bran cellulose. [Method] Taking the wheat bran as raw materials, and using orthogonal method, the effects of acid concentration, temperature, times and substrate concentration on the saccharification were investigated. [Result] The influence of temperature on the acid treatment of the saccharification of wheat bran cellulose was significant. Influences of acid concentration on the hydrolysis were distinct. Influences of time and substrate concentration were insignificant. The optimal pretreatment conditions were 1.5% of sulfuric acid concentration and 0.067 g/ml of substrate concentration at 100 ℃ in three hours. Under this condition, the sugar concentration was 38.137 mg/ml, and the hydrolysis rate reached 51.485%. [Conclusion] The study improved the saccharification of wheat bran cellulose, which provided theoretical basis for the application of wheat bran industrial process.展开更多
基金Supported by National Natural Fund Program(31071636)Anhui Science and Technology Bureau Program(1206C0805017/KJ2010A262)
文摘[Objective] The aim was to improve the saccharification of wheat bran cellulose. [Method] Taking the wheat bran as raw materials, and using orthogonal method, the effects of acid concentration, temperature, times and substrate concentration on the saccharification were investigated. [Result] The influence of temperature on the acid treatment of the saccharification of wheat bran cellulose was significant. Influences of acid concentration on the hydrolysis were distinct. Influences of time and substrate concentration were insignificant. The optimal pretreatment conditions were 1.5% of sulfuric acid concentration and 0.067 g/ml of substrate concentration at 100 ℃ in three hours. Under this condition, the sugar concentration was 38.137 mg/ml, and the hydrolysis rate reached 51.485%. [Conclusion] The study improved the saccharification of wheat bran cellulose, which provided theoretical basis for the application of wheat bran industrial process.
