Objective The aim of this study was to explore the ototoxicity of toluene in the early development of zebrafish embryos/larvae.Methods Zebrafish were utilized to explore the ototoxicity of toluene. Locomotion analysis...Objective The aim of this study was to explore the ototoxicity of toluene in the early development of zebrafish embryos/larvae.Methods Zebrafish were utilized to explore the ototoxicity of toluene. Locomotion analysis,immunofluorescence, and q PCR were used to understand the phenotypes and molecular mechanisms of toluene ototoxicity.Results The results demonstrated that at 2 mmol/L, toluene induced zebrafish larvae death at 120 hours post fertilization(hpf) at a rate of 25.79% and inhibited the rate of hatching at 72 hpf.Furthermore, toluene exposure inhibited the distance travelled and average swimming velocity of zebrafish larvae while increasing the frequency of movements. As shown by fluorescence staining of hair cells, toluene inhibited the formation of lateral line neuromasts and middle line 1(Ml1) neuromasts in 3 days post fertilization larvae in a concentration-dependent manner. Toluene altered the expression level of genes involved in ear development/function in zebrafish, among which the m RNA levels of cd164 l2,tekt3, and pcsk5 a were upregulated, while the level of otofb was downregulated, according to the q PCR results.Conclusion This study indicated that toluene may affect the development of both the inner ear and lateral line systems in zebrafish, while the lateral line system may be more sensitive to toluene than the inner ear.展开更多
Occupational exposure to organic solvents and the resulting health damage caused by organic solvent-induced occupational diseases(OSODs)have raised concern[1],as they have accounted for more than 70%of occupational-po...Occupational exposure to organic solvents and the resulting health damage caused by organic solvent-induced occupational diseases(OSODs)have raised concern[1],as they have accounted for more than 70%of occupational-poisoning cases in Guangdong province[2,3].However,there have been limited longitudinal studies examining OSOD patterns and temporal trends with a large sample in Guangdong.展开更多
Pneumoconiosis is a restrictive lung disease mostly caused by occupational exposure to dust,including silica dust,asbestos,and other fibers.Inhalable particles smaller than 10μm can enter the bronchioles,injure lung ...Pneumoconiosis is a restrictive lung disease mostly caused by occupational exposure to dust,including silica dust,asbestos,and other fibers.Inhalable particles smaller than 10μm can enter the bronchioles,injure lung cells,and induce lung fibrosis.This hard-to-cure disease places a substantial burden on patients and society[1].展开更多
基金funded by the Natural Science Foundation of Guangdong Province [grant number 2011B050700001]。
文摘Objective The aim of this study was to explore the ototoxicity of toluene in the early development of zebrafish embryos/larvae.Methods Zebrafish were utilized to explore the ototoxicity of toluene. Locomotion analysis,immunofluorescence, and q PCR were used to understand the phenotypes and molecular mechanisms of toluene ototoxicity.Results The results demonstrated that at 2 mmol/L, toluene induced zebrafish larvae death at 120 hours post fertilization(hpf) at a rate of 25.79% and inhibited the rate of hatching at 72 hpf.Furthermore, toluene exposure inhibited the distance travelled and average swimming velocity of zebrafish larvae while increasing the frequency of movements. As shown by fluorescence staining of hair cells, toluene inhibited the formation of lateral line neuromasts and middle line 1(Ml1) neuromasts in 3 days post fertilization larvae in a concentration-dependent manner. Toluene altered the expression level of genes involved in ear development/function in zebrafish, among which the m RNA levels of cd164 l2,tekt3, and pcsk5 a were upregulated, while the level of otofb was downregulated, according to the q PCR results.Conclusion This study indicated that toluene may affect the development of both the inner ear and lateral line systems in zebrafish, while the lateral line system may be more sensitive to toluene than the inner ear.
基金a Grant from Guangdong Province Hospital for Occupational Disease Prevention and TreatmentGuangdong Provincial Key Laboratory of Occupational Disease Prevention and Treatment[2017B030314152]+1 种基金a Grant from Science and Technology Science Foundation of Guangdong[2011B050700001,2013B021800176]a Grant from Guangdong medical scientific research fund[A2015150,C2016014]。
文摘Occupational exposure to organic solvents and the resulting health damage caused by organic solvent-induced occupational diseases(OSODs)have raised concern[1],as they have accounted for more than 70%of occupational-poisoning cases in Guangdong province[2,3].However,there have been limited longitudinal studies examining OSOD patterns and temporal trends with a large sample in Guangdong.
基金supported by Guangdong Provincial Science and Technology Plan Provincial International Cooperation Project[No.2011B050700001].
文摘Pneumoconiosis is a restrictive lung disease mostly caused by occupational exposure to dust,including silica dust,asbestos,and other fibers.Inhalable particles smaller than 10μm can enter the bronchioles,injure lung cells,and induce lung fibrosis.This hard-to-cure disease places a substantial burden on patients and society[1].
