Faba bean （Vicia faba L.）, one of the most important legumes in the world, evolved different types of cultivars due to its partial cross-pollination. The development of simple sequence repeat （SSR） markers from ex...Faba bean （Vicia faba L.）, one of the most important legumes in the world, evolved different types of cultivars due to its partial cross-pollination. The development of simple sequence repeat （SSR） markers from expressed sequence tags （EST） provided a useful tool for investigation of its genetic diversity. The purpose of the present study was to investigate the genetic diversity of faba bean from China and Europe using EST-SSR markers. 5 031 faba bean ESTs from the NCBI database were downloaded and assembled into 1 148 unigenes. A total of 107 microsatellites in 96 unigenes were identified, indicating that merely 8.36% of sequences contained SSRs. The most abundant SSR within faba bean was tri-nucleotide repeat motif, and among all the tri-nucleotide repeats, the motif AAG/CTT was the most abundant type. Based on these results, 11 EST-SSR markers were used to assess the genetic diversity of 29 faba bean cultivars from China and Europe with two to three alleles per locus. The polymorphism information content value ranged from 0.0644 to 0.4278 with an average of 0.2919. Principal coordinate analysis （PCA） and phylogenetic clustering based on these 11 EST-SSR markers distinguished these cultivars into different groups. The results indicated that faba bean in China had a narrow genetic basis, and the additional sources of genetic cultivars/accessions should be introduced to enhance the genetic variability. The results of this study proved that the EST-SSR marker is very effective in evaluation of faba bean germplasm.展开更多
基金supported by the Zhejiang Provincial Science and Technology Program, China (2007C32013)the Natural Science Foundation of Zhejiang Province, China (Y3090660)
文摘Faba bean （Vicia faba L.）, one of the most important legumes in the world, evolved different types of cultivars due to its partial cross-pollination. The development of simple sequence repeat （SSR） markers from expressed sequence tags （EST） provided a useful tool for investigation of its genetic diversity. The purpose of the present study was to investigate the genetic diversity of faba bean from China and Europe using EST-SSR markers. 5 031 faba bean ESTs from the NCBI database were downloaded and assembled into 1 148 unigenes. A total of 107 microsatellites in 96 unigenes were identified, indicating that merely 8.36% of sequences contained SSRs. The most abundant SSR within faba bean was tri-nucleotide repeat motif, and among all the tri-nucleotide repeats, the motif AAG/CTT was the most abundant type. Based on these results, 11 EST-SSR markers were used to assess the genetic diversity of 29 faba bean cultivars from China and Europe with two to three alleles per locus. The polymorphism information content value ranged from 0.0644 to 0.4278 with an average of 0.2919. Principal coordinate analysis （PCA） and phylogenetic clustering based on these 11 EST-SSR markers distinguished these cultivars into different groups. The results indicated that faba bean in China had a narrow genetic basis, and the additional sources of genetic cultivars/accessions should be introduced to enhance the genetic variability. The results of this study proved that the EST-SSR marker is very effective in evaluation of faba bean germplasm.
文摘目的系统评价肺癌患者并发肺部真菌感染的危险因素.方法系统检索CNKI、Wanfang、VIP、CBM、PubMed、Embase和The Cochrane Library,搜集有关肺癌患者并发肺部真菌感染危险因素的病例-对照研究和队列研究.检索时限从2007年1月-2022年10月.由2名研究者独立进行文献筛选、资料提取和质量评价,采用Rev Man 5.3软件进行Meta分析.结果共检索到24篇符合纳入与排除标准的文献,共计7349例肺癌患者.对18个潜在危险因素进行Meta分析,结果显示,年龄≥60岁、体力活动状态(PS)评分≥2分、吸烟史、高血压、糖尿病、贫血、TNM分期为Ⅲ-Ⅳ期、放疗、放化疗、侵入性操作、住院时间>14天、使用糖皮质激素和使用抗菌药物是肺癌患者发生肺部真菌感染的危险因素(均P<0.05).结论肺癌患者发生肺部真菌感染危险因素较多.早期识别肺癌患者发生肺部真菌感染的危险因素,并制定防治措施,有利于降低肺部真菌感染发病率.受纳入文献数量和质量限制,上述结论需要更多高质量研究予以验证.
