【目的】从岷山红三叶根际分离的109株促生菌,鉴定、筛选了具有生防能力的菌种资源并研究其生防特性,为生产应用提供服务。【方法】采用平板对峙法筛选优良生防菌株,对其产铁载体能力进行定性、定量测定和16S r RNA基因序列分析鉴定。...【目的】从岷山红三叶根际分离的109株促生菌,鉴定、筛选了具有生防能力的菌种资源并研究其生防特性,为生产应用提供服务。【方法】采用平板对峙法筛选优良生防菌株,对其产铁载体能力进行定性、定量测定和16S r RNA基因序列分析鉴定。【结果】平板对峙试验显示,109株促生菌中有8株促生菌对3种病原真菌(立枯丝核菌、黄瓜枯萎菌、西瓜尖镰孢菌)具有拮抗效果,分别是菌株MHS3、MHS7、MHS9、MHS27、MHS31、MHS38、MHS39和MHS48。其中,菌株MHS27、MHS31发酵液对立枯丝核菌抑菌率分别为40.1%、47.1%;菌株MHS7和MHS48发酵液对黄瓜枯萎菌抑菌率分别为26.1%、22.8%;菌株MHS27和MHS31发酵液对西瓜尖镰孢菌抑制率分别为34.0%、30.5%;产嗜铁素是生防菌(MHS7、MHS27、MHS31、MHS48)拮抗3种病原真菌的主要途径之一。经16S r RNA基因序列分析初步鉴定,菌株MHS7为特基拉芽孢杆菌(Bacillus tequilensis),菌株MHS27为溶蛋白芽孢杆菌(Bacillus proteolyticus),菌株MHS31为Pseudomonas paralactis,菌株MHS48为克雷伯氏菌(Klebsiella oxytoca)。【结论】从岷山红三叶根际分离筛选出4株具有优良生防作用的PGPR菌株,为今后生物菌肥应用推广提供了菌种资源支持。展开更多
Petroleum asphalt,an important by-product of the petrochemical industry,has diverse applications but often suffers from low industrial added value.Because of its low cost,high carbon content,and high polycyclic aromat...Petroleum asphalt,an important by-product of the petrochemical industry,has diverse applications but often suffers from low industrial added value.Because of its low cost,high carbon content,and high polycyclic aromatic hydrocarbon content,appropriate modification can increase its value and expand its energy storage applications.Current research progress on the common preparation methods of petroleum asphalt-based carbon materials,including template-assisted pyrolysis,molten salt treatment,activation,heteroatom doping,and pre-oxidation is reviewed,and its use in supercapacitors and alkali metal ion batteries,is also elaborated.Feasible solutions for the current problems with petroleum asphalt are proposed,with the aim of providing insights into its high value-added utilization.展开更多
文摘【目的】从岷山红三叶根际分离的109株促生菌,鉴定、筛选了具有生防能力的菌种资源并研究其生防特性,为生产应用提供服务。【方法】采用平板对峙法筛选优良生防菌株,对其产铁载体能力进行定性、定量测定和16S r RNA基因序列分析鉴定。【结果】平板对峙试验显示,109株促生菌中有8株促生菌对3种病原真菌(立枯丝核菌、黄瓜枯萎菌、西瓜尖镰孢菌)具有拮抗效果,分别是菌株MHS3、MHS7、MHS9、MHS27、MHS31、MHS38、MHS39和MHS48。其中,菌株MHS27、MHS31发酵液对立枯丝核菌抑菌率分别为40.1%、47.1%;菌株MHS7和MHS48发酵液对黄瓜枯萎菌抑菌率分别为26.1%、22.8%;菌株MHS27和MHS31发酵液对西瓜尖镰孢菌抑制率分别为34.0%、30.5%;产嗜铁素是生防菌(MHS7、MHS27、MHS31、MHS48)拮抗3种病原真菌的主要途径之一。经16S r RNA基因序列分析初步鉴定,菌株MHS7为特基拉芽孢杆菌(Bacillus tequilensis),菌株MHS27为溶蛋白芽孢杆菌(Bacillus proteolyticus),菌株MHS31为Pseudomonas paralactis,菌株MHS48为克雷伯氏菌(Klebsiella oxytoca)。【结论】从岷山红三叶根际分离筛选出4株具有优良生防作用的PGPR菌株,为今后生物菌肥应用推广提供了菌种资源支持。
文摘Petroleum asphalt,an important by-product of the petrochemical industry,has diverse applications but often suffers from low industrial added value.Because of its low cost,high carbon content,and high polycyclic aromatic hydrocarbon content,appropriate modification can increase its value and expand its energy storage applications.Current research progress on the common preparation methods of petroleum asphalt-based carbon materials,including template-assisted pyrolysis,molten salt treatment,activation,heteroatom doping,and pre-oxidation is reviewed,and its use in supercapacitors and alkali metal ion batteries,is also elaborated.Feasible solutions for the current problems with petroleum asphalt are proposed,with the aim of providing insights into its high value-added utilization.
