Blast disease caused by the fungus Magnaporthe grisea results in significant yield losses of cereal crops across the world.To date,very few regulatory genes contributing to blast resistance in grass species have been ...Blast disease caused by the fungus Magnaporthe grisea results in significant yield losses of cereal crops across the world.To date,very few regulatory genes contributing to blast resistance in grass species have been identified and the genetic basis of blast resistance in cereals remains elusive.Here,a core collection of foxtail millet(Setaria italica)containing 888 accessions was evaluated through inoculation with the blast strain HN-1 and a genome-wide association study(GWAS)was performed to detect regulators responsible for blast disease resistance in foxtail millet.The phenotypic variation of foxtail millet accessions inoculated with the blast strain HN-1 indicated that less than 1.60% of the samples were highly resistant,35.25% were moderately resistant,57.09% were moderately susceptible,and 6.08% were highly susceptible.The geographical pattern of blast-resistant samples revealed that a high proportion of resistant accessions were located in lower latitude regions where the foxtail millet growing season has higher rain precipitation.Using 720000 SNP markers covering the Setaria genome,GWAS showed that two genomic loci from chromosomes 2 and 9 were significantly associated with blast disease resistance in foxtail millet.Finally,eight putative genes were identified using rice blast-related transcriptomic data.The results of this work lay a foundation for the foxtail millet blast resistance biology and provide guidance for breeding practices in this promising crop species and other cereals.展开更多
基金This research was supported by the National Key R&D Program of China(2018YFD1000706 and 2018YFD1000700)the National Natural Science Foundation of China(31871630 and 31771807)+1 种基金the earmarked fund for China Agriculture Research System of MOF and MARA(CARS-06-13.5)the Agricultural Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences,and the Heilongjiang Academy of Agricultural Sciences Research Program,China(2018JJPY005).
文摘Blast disease caused by the fungus Magnaporthe grisea results in significant yield losses of cereal crops across the world.To date,very few regulatory genes contributing to blast resistance in grass species have been identified and the genetic basis of blast resistance in cereals remains elusive.Here,a core collection of foxtail millet(Setaria italica)containing 888 accessions was evaluated through inoculation with the blast strain HN-1 and a genome-wide association study(GWAS)was performed to detect regulators responsible for blast disease resistance in foxtail millet.The phenotypic variation of foxtail millet accessions inoculated with the blast strain HN-1 indicated that less than 1.60% of the samples were highly resistant,35.25% were moderately resistant,57.09% were moderately susceptible,and 6.08% were highly susceptible.The geographical pattern of blast-resistant samples revealed that a high proportion of resistant accessions were located in lower latitude regions where the foxtail millet growing season has higher rain precipitation.Using 720000 SNP markers covering the Setaria genome,GWAS showed that two genomic loci from chromosomes 2 and 9 were significantly associated with blast disease resistance in foxtail millet.Finally,eight putative genes were identified using rice blast-related transcriptomic data.The results of this work lay a foundation for the foxtail millet blast resistance biology and provide guidance for breeding practices in this promising crop species and other cereals.
