电离层总电子含量(Total Electron Content,TEC)的监测与预报是空间环境研究的重要内容,对卫星通讯和导航定位等有重要意义.TEC值影响因素较多,很难确定精确物理模型来对其进行预测.本文设计了基于注意力机制的LSTM模型(Att-LSTM),采用...电离层总电子含量(Total Electron Content,TEC)的监测与预报是空间环境研究的重要内容,对卫星通讯和导航定位等有重要意义.TEC值影响因素较多,很难确定精确物理模型来对其进行预测.本文设计了基于注意力机制的LSTM模型(Att-LSTM),采用过去24小时TEC观测数据对未来TEC进行预测.选择北半球东经100°上,每2.5°纬度选择一个位置,共计36个位置来验证本文提出模型的性能,并与主流的深度学习模型如DNN、RNN、LSTM进行对比实验.取得了如下成果:(1)在选定的36个地区未来2小时单点预测上,基于本文的Att-LSTM模型的TEC预测性能明显优于其他对比模型;(2)讨论了纬度对Att-LSTM预测未来2小时TEC值时性能的影响,发现在北纬0°到60°之间,Att-LSTM预测性能随着纬度的升高而略有降低,在北纬62.5°~87.5°之间,模型预测性能出现扰动,预测效果略差;(3)讨论了磁暴期和磁静期模型的预测性能,发现无论是磁暴期还是磁静期,本文模型预测性能均较好;(4)还讨论了对未来多时点预测效果,实验结果表明,本文所提出的模型对未来2、4个小时的预测拟合度R-Square均超过0.95,预测结果比较可靠,对未来6、8、10个小时预测拟合度最高为0.7934,预测拟合度R-Square下降迅速,预测结果不可靠.展开更多
BACKGROUND It is unclear that paired-related homeobox 1(PRRX1)induces epithelialmesenchymal transition(EMT)in oesophageal cancer and the specific function of PRRX1 in oesophageal cancer metastasis.AIM To assess the si...BACKGROUND It is unclear that paired-related homeobox 1(PRRX1)induces epithelialmesenchymal transition(EMT)in oesophageal cancer and the specific function of PRRX1 in oesophageal cancer metastasis.AIM To assess the signicance of PRRX1 expression and investigate the mechanism of EMT in oesophageal cancer metastasis.METHODS Detect the expression of PRRX1 by immunohistochemistry in oesophageal tumour tissues and adjacent normal oesophageal tissues;the PRRX1 short hairpin RNA(shRNA)or blank vector lentiviral gene delivery system was transfected into cells;cell proliferation assay,soft agar colony formation assays,cell invasion and migration assays and animal studies were used to observe cells biological characteristics In vitro and in vivo;XAV939 and LiCl were used to alter the activity of Wnt/β-catenin pathway.Immunofluorescence staining and western blot analysis were used to detect protein expression of EMT markers and Wnt/β-catenin pathway.RESULTS PRRX1 is expressed at high levels in oesophageal cancer specimens and is closely related to tumour metastasis in patients with oesophageal cancer.Regulation of PRRX1 expression might exert obvious effects on cell proliferation,especially the migration and invasion of oesophageal cancer cells.Moreover,silencing PRRX1 expression using a shRNA produced the opposite effects.In addition,when PRRX1 was overexpressed,inhibition of the Wnt/β-catenin pathway with XAV939 negated the effect of PRRX1 on EMT,whereas when PRRX1 was downregulated,activation of the Wnt/β-catenin pathway with LiCl impaired the effect on EMT.CONCLUSION PRRX1 is upregulated in oesophageal cancer is closely correlated with cancer metastasis.Additionally,PRRX1 induces EMT in oesophageal cancer metastasis through activation of Wnt/β-catenin signalling.展开更多
【目的】克隆黄鳝csf1r基因,并对其时空表达特性进行分析,为探明csf1r基因在黄鳝不同体色形成中的作用奠定基础。【方法】采用RACEs(Rapid-amplification of cDNA ends)技术从黄鳝皮肤cDNAs中克隆得到csf1r基因的全长cDNA序列,对其编码...【目的】克隆黄鳝csf1r基因,并对其时空表达特性进行分析,为探明csf1r基因在黄鳝不同体色形成中的作用奠定基础。【方法】采用RACEs(Rapid-amplification of cDNA ends)技术从黄鳝皮肤cDNAs中克隆得到csf1r基因的全长cDNA序列,对其编码蛋白进行生物信息学分析。采用实时荧光定量PCR(qRT-PCR)方法检测csf1r基因在黄鳝不同组织、不同发育时期胚胎或个体及3种体色黄鳝(黄黑斑鳝、碎花斑鳝和隐花斑鳝)皮肤和肾脏中的相对表达量,分析该基因的表达特征。测定3种体色黄鳝肝脏中的碱性磷酸酶(AKP)、超氧化物歧化酶(SOD)活性和总抗氧化能力(T-AOC)。【结果】黄鳝csf1r cDNA序列全长为4430 bp(GenBank收录号:OP589303),其编码区长度为2937 bp,编码978个氨基酸,存在免疫球蛋白结构域和蛋白激酶催化结构域2个保守结构域。荧光定量PCR结果表明,csf1r基因在黄鳝脑、精巢、卵巢、肠、心脏、肾脏、肝脏、肌肉、皮肤和脾脏等组织中均有表达,在脾脏和心脏中表达量较高,其次是肾脏、皮肤和肌肉,卵巢中表达量最低;csf1r在胚胎眼晶体形成期开始大量表达,显微观察发现该时期胚胎的躯干上开始有色素颗粒出现。在3种体色黄鳝皮肤和肾脏中,csf1r基因在黄黑斑鳝的皮肤中表达量最低,而在其肾脏中表达量最高。3种体色黄鳝肝脏氧化应激指标测定结果发现,黄黑斑鳝肝脏中的碱性磷酸酶(AKP)、超氧化物歧化酶(SOD)活性和总抗氧化能力比其他2种体色黄鳝高,但是差异未达显著水平。【结论】csf1r基因可能不仅参与了黄鳝体色的形成,还与黄鳝非特异性免疫相关。展开更多
BACKGROUND:Heatstroke has become a common emergency event in hospitals.Procalcitonin(PCT)is used as a biomarker of infection in the emergency department(ED),but its role in rhabdomyolysis(RM)following exertional heats...BACKGROUND:Heatstroke has become a common emergency event in hospitals.Procalcitonin(PCT)is used as a biomarker of infection in the emergency department(ED),but its role in rhabdomyolysis(RM)following exertional heatstroke(EHS)remains unclear.METHODS:A retrospective cohort study enrolled patients with EHS from the intensive care unit(ICU).We collected RM biomarkers,inflammation markers,critical disease scores at admission,24 h,48 h,and discharge,and 90-day mortality.Correlation analysis,linear regression and curve fi tting were used to identify the relationship between PCT and RM.RESULTS:A total of 162 patients were recruited and divided into RM(n=56)and non-RM(n=106)groups.PCT was positively correlated with myoglobin(Mb),acute hepatic injury,disseminated intravascular coagulation(DIC),Sequential Organ Failure Assessment(SOFA)score,and Acute Physiology and Chronic Health Evaluation II(APACHE II)score,with correlation coefficients of 0.214,0.237,0.285,0.454,and 0.368,respectively(all P<0.05).Interestingly,the results of curve fi tting revealed a nonlinear relationship between PCT and RM,and a two-piecewise linear regression model showed that PCT was related to RM with an odds ratio of 1.3 and a cut-off of<4.6 ng/mL.Survival analysis revealed that RM was associated with higher mortality compared to non-RM cases(P=0.0093).CONCLUSION:High serum PCT concentrations are associated with RM after EHS in critically ill patients.Elevated PCT concentrations should be interpreted cautiously in patients with EHS in the ED.展开更多
文摘电离层总电子含量(Total Electron Content,TEC)的监测与预报是空间环境研究的重要内容,对卫星通讯和导航定位等有重要意义.TEC值影响因素较多,很难确定精确物理模型来对其进行预测.本文设计了基于注意力机制的LSTM模型(Att-LSTM),采用过去24小时TEC观测数据对未来TEC进行预测.选择北半球东经100°上,每2.5°纬度选择一个位置,共计36个位置来验证本文提出模型的性能,并与主流的深度学习模型如DNN、RNN、LSTM进行对比实验.取得了如下成果:(1)在选定的36个地区未来2小时单点预测上,基于本文的Att-LSTM模型的TEC预测性能明显优于其他对比模型;(2)讨论了纬度对Att-LSTM预测未来2小时TEC值时性能的影响,发现在北纬0°到60°之间,Att-LSTM预测性能随着纬度的升高而略有降低,在北纬62.5°~87.5°之间,模型预测性能出现扰动,预测效果略差;(3)讨论了磁暴期和磁静期模型的预测性能,发现无论是磁暴期还是磁静期,本文模型预测性能均较好;(4)还讨论了对未来多时点预测效果,实验结果表明,本文所提出的模型对未来2、4个小时的预测拟合度R-Square均超过0.95,预测结果比较可靠,对未来6、8、10个小时预测拟合度最高为0.7934,预测拟合度R-Square下降迅速,预测结果不可靠.
文摘BACKGROUND It is unclear that paired-related homeobox 1(PRRX1)induces epithelialmesenchymal transition(EMT)in oesophageal cancer and the specific function of PRRX1 in oesophageal cancer metastasis.AIM To assess the signicance of PRRX1 expression and investigate the mechanism of EMT in oesophageal cancer metastasis.METHODS Detect the expression of PRRX1 by immunohistochemistry in oesophageal tumour tissues and adjacent normal oesophageal tissues;the PRRX1 short hairpin RNA(shRNA)or blank vector lentiviral gene delivery system was transfected into cells;cell proliferation assay,soft agar colony formation assays,cell invasion and migration assays and animal studies were used to observe cells biological characteristics In vitro and in vivo;XAV939 and LiCl were used to alter the activity of Wnt/β-catenin pathway.Immunofluorescence staining and western blot analysis were used to detect protein expression of EMT markers and Wnt/β-catenin pathway.RESULTS PRRX1 is expressed at high levels in oesophageal cancer specimens and is closely related to tumour metastasis in patients with oesophageal cancer.Regulation of PRRX1 expression might exert obvious effects on cell proliferation,especially the migration and invasion of oesophageal cancer cells.Moreover,silencing PRRX1 expression using a shRNA produced the opposite effects.In addition,when PRRX1 was overexpressed,inhibition of the Wnt/β-catenin pathway with XAV939 negated the effect of PRRX1 on EMT,whereas when PRRX1 was downregulated,activation of the Wnt/β-catenin pathway with LiCl impaired the effect on EMT.CONCLUSION PRRX1 is upregulated in oesophageal cancer is closely correlated with cancer metastasis.Additionally,PRRX1 induces EMT in oesophageal cancer metastasis through activation of Wnt/β-catenin signalling.
基金funded by the National Natural Science Foundation of China(82072143,81873943,82360903)Sanming Project of Medicine in Shenzhen(SZSM20162011)+3 种基金Shenzhen Science and Technology Innovation Commission(JCYJ20190806163603504)Shenzhen Second People’s Hospital Clinical Research Fund of Guangdong Province High-level Hospital Construction Project(20203357014,2023xgyj3357001,2023yjlcyj022)Guizhou Science and Technology Planning Project(Guizhou Science and Technology Cooperation Support[2021]General 413)and PhD Start-up Fund(GYZYYFY-BS-2023[09]).
文摘BACKGROUND:Heatstroke has become a common emergency event in hospitals.Procalcitonin(PCT)is used as a biomarker of infection in the emergency department(ED),but its role in rhabdomyolysis(RM)following exertional heatstroke(EHS)remains unclear.METHODS:A retrospective cohort study enrolled patients with EHS from the intensive care unit(ICU).We collected RM biomarkers,inflammation markers,critical disease scores at admission,24 h,48 h,and discharge,and 90-day mortality.Correlation analysis,linear regression and curve fi tting were used to identify the relationship between PCT and RM.RESULTS:A total of 162 patients were recruited and divided into RM(n=56)and non-RM(n=106)groups.PCT was positively correlated with myoglobin(Mb),acute hepatic injury,disseminated intravascular coagulation(DIC),Sequential Organ Failure Assessment(SOFA)score,and Acute Physiology and Chronic Health Evaluation II(APACHE II)score,with correlation coefficients of 0.214,0.237,0.285,0.454,and 0.368,respectively(all P<0.05).Interestingly,the results of curve fi tting revealed a nonlinear relationship between PCT and RM,and a two-piecewise linear regression model showed that PCT was related to RM with an odds ratio of 1.3 and a cut-off of<4.6 ng/mL.Survival analysis revealed that RM was associated with higher mortality compared to non-RM cases(P=0.0093).CONCLUSION:High serum PCT concentrations are associated with RM after EHS in critically ill patients.Elevated PCT concentrations should be interpreted cautiously in patients with EHS in the ED.
