Based on morphological,molecular biological,and molecular systematic studies,we describe here a new species of Malus from Yunnan,China.We compared the morphology of this new species,Malus shizongensis Liu sp.nov,with ...Based on morphological,molecular biological,and molecular systematic studies,we describe here a new species of Malus from Yunnan,China.We compared the morphology of this new species,Malus shizongensis Liu sp.nov,with three Malus species,including M.hupehensis,M.baccata,and M.micromalus.Although the appearance of M.shizongensis was similar to these three species,it differed in height,branch color,branch hair,and flower color.To better identify the taxonomy of this new species,genome of M.shizongensis and that of seven Malus species,including M.prunifolia,M.sylvestris,M.sieversi,M.hupehensis,M.baccata,M.robusta,and M.micromalus were analyzed.A phylogenetic tree based on genome analysis indicated that M.shizongensis was close to M.hupehensis.Furthermore,M.shizongensis had its species-specific SNPs,and the number of species-specific SNPs was similar to that of three close species(M.hupehensis,M.baccata,and M.micromalus).Based on the above information,we named this new species as M.shizongensis Liu sp.nov.展开更多
Background Endometriosis is a common gynecological disease. This study aimed to screen proteins that were expressed differently in patients with endometriosis versus normal controls using proteomic techniques, surface...Background Endometriosis is a common gynecological disease. This study aimed to screen proteins that were expressed differently in patients with endometriosis versus normal controls using proteomic techniques, surface-enhanced laser desorption/ionization time-of-flight mass spectrometry （SELDI-TOF-MS）.Methods Protein chip SELDI-TOF-MS combines the advantages of microarray and mass spectrometry, and can screen latent markers in sera of patients with endometriosis. Serum samples from patients and normal volunteers were analyzed by SELDI-TOF-MS. Results After comparing the serum protein spectra of 36 patients with 24 normal controls, 24 differently expressed potential biomarkers （P 〈0.01） were identified. Using Biomarker Pattern software, we established a tree model of the 60 serum protein spectra. When using the three bJomarkers to classify the samples, the sensitivity for diagnosing endometriosis was 91.7%, specificity was 95.8%, and coincidence rate was 93.3%. Then we used serum samples from 12 patients and 8 normal controls to validate the tree model and report the sensitivity for diagnosing endometriosis was 91.7%, specificity was 75%, and coincidence rate was 85%. Conclusions SELDI-TOF-MS may be a useful tool in high-risk population screening for endometriosis. The identification and application of the biomarkers need to further study.展开更多
基金Supported by the Shaanxi Provincial Science and Technology Project,China(K3310216042,K3360217106).
文摘Based on morphological,molecular biological,and molecular systematic studies,we describe here a new species of Malus from Yunnan,China.We compared the morphology of this new species,Malus shizongensis Liu sp.nov,with three Malus species,including M.hupehensis,M.baccata,and M.micromalus.Although the appearance of M.shizongensis was similar to these three species,it differed in height,branch color,branch hair,and flower color.To better identify the taxonomy of this new species,genome of M.shizongensis and that of seven Malus species,including M.prunifolia,M.sylvestris,M.sieversi,M.hupehensis,M.baccata,M.robusta,and M.micromalus were analyzed.A phylogenetic tree based on genome analysis indicated that M.shizongensis was close to M.hupehensis.Furthermore,M.shizongensis had its species-specific SNPs,and the number of species-specific SNPs was similar to that of three close species(M.hupehensis,M.baccata,and M.micromalus).Based on the above information,we named this new species as M.shizongensis Liu sp.nov.
基金This study was supported by the grants from Beijing Municipal Science ＆ Technology Commission （No.H030930040230） and the National Natural Science Foundation of China （No.30772319）.
文摘Background Endometriosis is a common gynecological disease. This study aimed to screen proteins that were expressed differently in patients with endometriosis versus normal controls using proteomic techniques, surface-enhanced laser desorption/ionization time-of-flight mass spectrometry （SELDI-TOF-MS）.Methods Protein chip SELDI-TOF-MS combines the advantages of microarray and mass spectrometry, and can screen latent markers in sera of patients with endometriosis. Serum samples from patients and normal volunteers were analyzed by SELDI-TOF-MS. Results After comparing the serum protein spectra of 36 patients with 24 normal controls, 24 differently expressed potential biomarkers （P 〈0.01） were identified. Using Biomarker Pattern software, we established a tree model of the 60 serum protein spectra. When using the three bJomarkers to classify the samples, the sensitivity for diagnosing endometriosis was 91.7%, specificity was 95.8%, and coincidence rate was 93.3%. Then we used serum samples from 12 patients and 8 normal controls to validate the tree model and report the sensitivity for diagnosing endometriosis was 91.7%, specificity was 75%, and coincidence rate was 85%. Conclusions SELDI-TOF-MS may be a useful tool in high-risk population screening for endometriosis. The identification and application of the biomarkers need to further study.
