A physical simulation method with a combination of dynamic displacement and imbibition was established by integrating nuclear magnetic resonance(NMR)and CT scanning.The microscopic production mechanism of tight/shale ...A physical simulation method with a combination of dynamic displacement and imbibition was established by integrating nuclear magnetic resonance(NMR)and CT scanning.The microscopic production mechanism of tight/shale oil in pore throat by dynamic imbibition and the influencing factors on the development effect of dynamic imbibition were analyzed.The dynamic seepage process of fracking-soaking-backflow-production integration was simulated,which reveals the dynamic production characteristics at different development stages and their contribution to enhancing oil recovery(EOR).The seepage of tight/shale reservoirs can be divided into three stages:strong displacement and weak imbibition as oil produced rapidly by displacement from macropores and fractures,weak displacement and strong imbibition as oil produced slowly by reverse imbibition from small pores,and weak displacement and weak imbibition at dynamic equilibrium.The greater displacement pressure results in the higher displacement recovery and the lower imbibition recovery.However,if the displacement pressure is too high,the injected water is easy to break through the front and reduce the recovery degree.The higher the permeability,the greater the imbibition and displacement recovery,the shorter the time of imbibition balance,and the higher the final recovery.The fractures can effectively increase the imbibition contact area between matrix and water,reduce the oil-water seepage resistance,promote the oil-water displacement between matrix and fracture,and improve the oil displacement rate and recovery of the matrix.The soaking after fracturing is beneficial to the imbibition replacement and energy storage of the fluid;also,the effective use of the carrying of the backflow fluid and the displacement in the mining stage is the key to enhancing oil recovery.展开更多
目的分析成年高尔夫球员腰部损伤的风险因素及健康与康复干预策略的证据。方法采用主题词检索方法,在PubMed、Web of Science、EBSCO、Scopus、Cochrane图书馆、中国知网、维普和万方数据库,检索成年高尔夫球员腰部损伤的相关文献,检索...目的分析成年高尔夫球员腰部损伤的风险因素及健康与康复干预策略的证据。方法采用主题词检索方法,在PubMed、Web of Science、EBSCO、Scopus、Cochrane图书馆、中国知网、维普和万方数据库,检索成年高尔夫球员腰部损伤的相关文献,检索时间为建库至2024年4月1日。提取作者、国家、发表时间、研究对象、腰部损伤风险因素和干预策略,并进行Scoping综述。结果最终纳入英文文献9篇,来自美国、澳大利亚、韩国、葡萄牙和南非5个国家,涉及237例高尔夫球员,研究类型涉及调查研究3篇、前瞻性纵向队列研究1篇和随机对照试验或准实验设计5篇。研究对象包括职业高尔夫球员和业余高尔夫球员。造成腰部损伤风险因素主要有过量重复的不规范挥杆技术动作造成腰椎过度扭转和腰部肌群过度使用,腹直肌、竖脊肌和背阔肌激活模式异常导致无法维持大强度挥杆动作中的脊柱稳定,以及躯干与髋关节旋转功能受限造成挥杆动作中腰椎过度补偿。腰部损伤健康与康复干预策略有充分利用肌电、超声生物反馈技术,加强对腰部薄弱肌群和挥杆技术动作的筛查,进一步优化训练负荷和规范挥杆技术动作,加强躯干和髋关节的功能性训练,加强腰腹、核心肌群和深层肌群的力量训练。结论成年高尔夫球员腰部损伤的风险因素主要涉及过量重复的不规范挥杆技术动作、腰腹与核心肌群力量不足、躯干与髋关节功能受限。有效的干预策略包括充分利用肌电、超声生物反馈技术优化训练负荷,规范挥杆技术动作、加强躯干和髋关节的功能训练、增强腰腹,核心肌群与深层肌群的力量训练。这些策略有助于减少高尔夫球员的腰部损伤风险,提升运动表现和身心健康。展开更多
The main methods of coalbed methane(CBM)development are drainage and depressurization,and a precise prediction of coal reservoir pressure is thus crucial for the evaluation of reservoir potentials and the formulation ...The main methods of coalbed methane(CBM)development are drainage and depressurization,and a precise prediction of coal reservoir pressure is thus crucial for the evaluation of reservoir potentials and the formulation of reasonable development plans.This work established a new reservoir pressure prediction model based on the material balance equation(MBE)of coal reservoir,which considers the self-regulating effects of coal reservoirs and the dynamic change of equivalent drainage area(EDA).According to the proposed model,the reservoir pressure can be predicted based on reservoir condition data and the actual production data of a single well.Compared with traditional reservoir pressure prediction models which regard EDA as a fixed value,the proposed model can better predict the average pressure of reservoirs.Moreover,orthogonal experiments were designed to evaluate the sensitivity of reservoir parameters on the reservoir pressure prediction results of this proposed model.The results show that the saturation of irreducible water is the most sensitive parameter,followed by Langmuir volume and reservoir porosity,and Langmuir pressure is the least sensitive parameter.In addition,the pressure drop of reservoirs is negatively correlated with the saturation of irreducible water and the Langmuir volume,while it is positively correlated with porosity.This work analyzed the reservoir pressure drop characteristics of the CBM wells in the Shizhuangnan Block of the Qinshui Basin,and the results show that the CBM reservoir depressurization can be divided into three types,i.e.,rapidly drop type,medium-term stability type,and slowly drop type.The drainage features of wells were reasonably interpreted based on the comprehensive analysis of the reservoir depressurization type;the latter was coupled to the corresponding permeability dynamic change characteristics,eventually proving the applicability of the proposed model.展开更多
目的:探讨核仁纺锤体相关蛋白1(nucleolar and spindle-associated protein 1,NUSAP1)在食管癌(esophageal carci⁃noma,ESCA)中的表达情况、临床意义及功能机制,为ESCA的早期诊断和治疗提供新思路。方法:从GEPIA2和TIMER数据库下载ESCA...目的:探讨核仁纺锤体相关蛋白1(nucleolar and spindle-associated protein 1,NUSAP1)在食管癌(esophageal carci⁃noma,ESCA)中的表达情况、临床意义及功能机制,为ESCA的早期诊断和治疗提供新思路。方法:从GEPIA2和TIMER数据库下载ESCA样本与癌旁样本,进行NUSAP1的表达分析,STRING和Cytoscape软件进行NUSAP1相关基因的PPI网络构建,并进行GO、KEGG及GSEA富集分析。TIMER数据库被用来分析NUSAP1与免疫细胞的相关性。最后通过RT-PCR、Western blot和免疫组化验证NUSAP1在ESCA组织和细胞中的表达,使用慢病毒包被的小干扰RNA转染ESCA细胞,进行NUSAP1的表达敲减。通过CKK-8和Ki67免疫荧光评估NUSAP1对ESCA细胞增殖的影响。结果:NUSAP1在ESCA组织和细胞中高表达。NUSAP1的表达与CDK1、B细胞和巨噬细胞浸润正相关,而与CD4+T细胞、CD8+T细胞、中性粒细胞和树突状细胞浸润负相关。GSEA分析发现NUSAP1的高表达组主要富集于Wnt/β-catenin信号通路、过氧化物酶通路、PI3K/Akt/mTOR信号通路。在体外,敲减NUSAP1后抑制了ESCA细胞增殖。结论:NUSAP1在ESCA中高表达,并与ESCA细胞增殖和免疫细胞浸润密切相关。因此,NUSAP1可能有助于ESCA的诊断和治疗。展开更多
Inflorescence structure of rice,including the number and length of branches,and the density of the spikelet,can greatly affect the number of grains per panicle,which is one of the key factors in yield compositions.Her...Inflorescence structure of rice,including the number and length of branches,and the density of the spikelet,can greatly affect the number of grains per panicle,which is one of the key factors in yield compositions.Here we identified five allelic mutants sb1-1/2/3/4/5 that related to branch development of rice.In these mutants,the branch meristem fate was prolonged sharply,resulting in delay of transition from branches to spikelets,and then increased the numbers of branches and spikelets per panicle.SB1 encodes a nuclear RING-like domain protein of SHI/LRP/SRS family and strongly expressed in branch meristems.The results of protein interaction and chromatin immunoprecipitation further suggested that SB1 directly repressed the expression of DEP1,TAW1,MOC1 and IPA1 by interacting with a co-repressor complex to affect acetylation level of histone H3 on target regions.Thus,we proposed that SB1 is a transcription repressor of branch meristem activity by widely and negatively regulating a series of genes that maintain branch meristem fate.展开更多
基金Supported by the PetroChina Science and Technology Major Project(2021-117)PetroChina CCUS Major Science and Technology Project(2021ZZ01-03)。
文摘A physical simulation method with a combination of dynamic displacement and imbibition was established by integrating nuclear magnetic resonance(NMR)and CT scanning.The microscopic production mechanism of tight/shale oil in pore throat by dynamic imbibition and the influencing factors on the development effect of dynamic imbibition were analyzed.The dynamic seepage process of fracking-soaking-backflow-production integration was simulated,which reveals the dynamic production characteristics at different development stages and their contribution to enhancing oil recovery(EOR).The seepage of tight/shale reservoirs can be divided into three stages:strong displacement and weak imbibition as oil produced rapidly by displacement from macropores and fractures,weak displacement and strong imbibition as oil produced slowly by reverse imbibition from small pores,and weak displacement and weak imbibition at dynamic equilibrium.The greater displacement pressure results in the higher displacement recovery and the lower imbibition recovery.However,if the displacement pressure is too high,the injected water is easy to break through the front and reduce the recovery degree.The higher the permeability,the greater the imbibition and displacement recovery,the shorter the time of imbibition balance,and the higher the final recovery.The fractures can effectively increase the imbibition contact area between matrix and water,reduce the oil-water seepage resistance,promote the oil-water displacement between matrix and fracture,and improve the oil displacement rate and recovery of the matrix.The soaking after fracturing is beneficial to the imbibition replacement and energy storage of the fluid;also,the effective use of the carrying of the backflow fluid and the displacement in the mining stage is the key to enhancing oil recovery.
文摘目的分析成年高尔夫球员腰部损伤的风险因素及健康与康复干预策略的证据。方法采用主题词检索方法,在PubMed、Web of Science、EBSCO、Scopus、Cochrane图书馆、中国知网、维普和万方数据库,检索成年高尔夫球员腰部损伤的相关文献,检索时间为建库至2024年4月1日。提取作者、国家、发表时间、研究对象、腰部损伤风险因素和干预策略,并进行Scoping综述。结果最终纳入英文文献9篇,来自美国、澳大利亚、韩国、葡萄牙和南非5个国家,涉及237例高尔夫球员,研究类型涉及调查研究3篇、前瞻性纵向队列研究1篇和随机对照试验或准实验设计5篇。研究对象包括职业高尔夫球员和业余高尔夫球员。造成腰部损伤风险因素主要有过量重复的不规范挥杆技术动作造成腰椎过度扭转和腰部肌群过度使用,腹直肌、竖脊肌和背阔肌激活模式异常导致无法维持大强度挥杆动作中的脊柱稳定,以及躯干与髋关节旋转功能受限造成挥杆动作中腰椎过度补偿。腰部损伤健康与康复干预策略有充分利用肌电、超声生物反馈技术,加强对腰部薄弱肌群和挥杆技术动作的筛查,进一步优化训练负荷和规范挥杆技术动作,加强躯干和髋关节的功能性训练,加强腰腹、核心肌群和深层肌群的力量训练。结论成年高尔夫球员腰部损伤的风险因素主要涉及过量重复的不规范挥杆技术动作、腰腹与核心肌群力量不足、躯干与髋关节功能受限。有效的干预策略包括充分利用肌电、超声生物反馈技术优化训练负荷,规范挥杆技术动作、加强躯干和髋关节的功能训练、增强腰腹,核心肌群与深层肌群的力量训练。这些策略有助于减少高尔夫球员的腰部损伤风险,提升运动表现和身心健康。
基金financially supported by the National Natural Science Foundation of China (grants No. 41772159/D0208, No. 41872178)the National Science and Technology Major Project of China (grant No. 2017ZX05064003)
文摘The main methods of coalbed methane(CBM)development are drainage and depressurization,and a precise prediction of coal reservoir pressure is thus crucial for the evaluation of reservoir potentials and the formulation of reasonable development plans.This work established a new reservoir pressure prediction model based on the material balance equation(MBE)of coal reservoir,which considers the self-regulating effects of coal reservoirs and the dynamic change of equivalent drainage area(EDA).According to the proposed model,the reservoir pressure can be predicted based on reservoir condition data and the actual production data of a single well.Compared with traditional reservoir pressure prediction models which regard EDA as a fixed value,the proposed model can better predict the average pressure of reservoirs.Moreover,orthogonal experiments were designed to evaluate the sensitivity of reservoir parameters on the reservoir pressure prediction results of this proposed model.The results show that the saturation of irreducible water is the most sensitive parameter,followed by Langmuir volume and reservoir porosity,and Langmuir pressure is the least sensitive parameter.In addition,the pressure drop of reservoirs is negatively correlated with the saturation of irreducible water and the Langmuir volume,while it is positively correlated with porosity.This work analyzed the reservoir pressure drop characteristics of the CBM wells in the Shizhuangnan Block of the Qinshui Basin,and the results show that the CBM reservoir depressurization can be divided into three types,i.e.,rapidly drop type,medium-term stability type,and slowly drop type.The drainage features of wells were reasonably interpreted based on the comprehensive analysis of the reservoir depressurization type;the latter was coupled to the corresponding permeability dynamic change characteristics,eventually proving the applicability of the proposed model.
文摘目的:探讨核仁纺锤体相关蛋白1(nucleolar and spindle-associated protein 1,NUSAP1)在食管癌(esophageal carci⁃noma,ESCA)中的表达情况、临床意义及功能机制,为ESCA的早期诊断和治疗提供新思路。方法:从GEPIA2和TIMER数据库下载ESCA样本与癌旁样本,进行NUSAP1的表达分析,STRING和Cytoscape软件进行NUSAP1相关基因的PPI网络构建,并进行GO、KEGG及GSEA富集分析。TIMER数据库被用来分析NUSAP1与免疫细胞的相关性。最后通过RT-PCR、Western blot和免疫组化验证NUSAP1在ESCA组织和细胞中的表达,使用慢病毒包被的小干扰RNA转染ESCA细胞,进行NUSAP1的表达敲减。通过CKK-8和Ki67免疫荧光评估NUSAP1对ESCA细胞增殖的影响。结果:NUSAP1在ESCA组织和细胞中高表达。NUSAP1的表达与CDK1、B细胞和巨噬细胞浸润正相关,而与CD4+T细胞、CD8+T细胞、中性粒细胞和树突状细胞浸润负相关。GSEA分析发现NUSAP1的高表达组主要富集于Wnt/β-catenin信号通路、过氧化物酶通路、PI3K/Akt/mTOR信号通路。在体外,敲减NUSAP1后抑制了ESCA细胞增殖。结论:NUSAP1在ESCA中高表达,并与ESCA细胞增殖和免疫细胞浸润密切相关。因此,NUSAP1可能有助于ESCA的诊断和治疗。
基金supported by the National Natural Science Foundation of China(Grant No.31971919)the National Key Program for Research and Development of China(Grant No.2017YFD0100202)+1 种基金the Project Sponsored by Natural Science Foundation of Chongqing,China(Grant No.cstc2020jcyjjqX0020)Chongqing Graduate Research and Innovation Project funding in China(Grant No.CYS20123)。
文摘Inflorescence structure of rice,including the number and length of branches,and the density of the spikelet,can greatly affect the number of grains per panicle,which is one of the key factors in yield compositions.Here we identified five allelic mutants sb1-1/2/3/4/5 that related to branch development of rice.In these mutants,the branch meristem fate was prolonged sharply,resulting in delay of transition from branches to spikelets,and then increased the numbers of branches and spikelets per panicle.SB1 encodes a nuclear RING-like domain protein of SHI/LRP/SRS family and strongly expressed in branch meristems.The results of protein interaction and chromatin immunoprecipitation further suggested that SB1 directly repressed the expression of DEP1,TAW1,MOC1 and IPA1 by interacting with a co-repressor complex to affect acetylation level of histone H3 on target regions.Thus,we proposed that SB1 is a transcription repressor of branch meristem activity by widely and negatively regulating a series of genes that maintain branch meristem fate.
