Fishes represent the highest diversity of vertebrates; however, our understanding of the compositions and functions of their gut microbiota is limited. In this study, we provided the first insight into the gut microbi...Fishes represent the highest diversity of vertebrates; however, our understanding of the compositions and functions of their gut microbiota is limited. In this study, we provided the first insight into the gut microbiota of the herbivorous fish Siganus eanalieulatus by using three molecular ecology techniques based on the 16S rRNA genes （denaturing gradient gel electrophoresis, clone library construction, and high- throughput Illumina sequencing）, and the Illumina sequencing technique is suggested here due to its higher overall coverage of the total 16S rRNA genes. A core gut microbiota of 29 bacterial groups, covering 〉99.9% of the total bacterial community, was found to be dominated by Proteobacteria and Firmicutes in fish fed three different diets with/without the supplementation of Ulva pertusa and non-starch polysaccharide （NSP） enzymes （cellulase, xylanase, and [3-glucanase）. Diverse potential NSP-degrading bacteria and probiotics （e.g., Ruminococcus, Clostridium and Lachnospiraceae） were detected in the intestine of the fish fed U. pertusa, suggesting that these microorganisms likely participated in the degradation of NSPs derived from U. pertusa. This study supports our previous conclusion that U. pertusa-based diets are suitable for the production ofS. eanaliculatus with lower costs without compromising quality.展开更多
A systematic experimental study on the self quenching streamer discharge has been performed.The dependence of the charge developed in the discharge on the high voltage can be approximated by a linear relation.The conc...A systematic experimental study on the self quenching streamer discharge has been performed.The dependence of the charge developed in the discharge on the high voltage can be approximated by a linear relation.The concentration of quenching gas and anode diameter have little influence on the slope of this linear relation.展开更多
基金Supported by the National Natural Science Foundation of China(Nos.41641053,41376176,41276179)the Team Project of Natural Science Foundation of Guangdong Province(No.S2011030005257)the China Postdoctoral Science Foundation(No.2016M602501)
文摘Fishes represent the highest diversity of vertebrates; however, our understanding of the compositions and functions of their gut microbiota is limited. In this study, we provided the first insight into the gut microbiota of the herbivorous fish Siganus eanalieulatus by using three molecular ecology techniques based on the 16S rRNA genes （denaturing gradient gel electrophoresis, clone library construction, and high- throughput Illumina sequencing）, and the Illumina sequencing technique is suggested here due to its higher overall coverage of the total 16S rRNA genes. A core gut microbiota of 29 bacterial groups, covering 〉99.9% of the total bacterial community, was found to be dominated by Proteobacteria and Firmicutes in fish fed three different diets with/without the supplementation of Ulva pertusa and non-starch polysaccharide （NSP） enzymes （cellulase, xylanase, and [3-glucanase）. Diverse potential NSP-degrading bacteria and probiotics （e.g., Ruminococcus, Clostridium and Lachnospiraceae） were detected in the intestine of the fish fed U. pertusa, suggesting that these microorganisms likely participated in the degradation of NSPs derived from U. pertusa. This study supports our previous conclusion that U. pertusa-based diets are suitable for the production ofS. eanaliculatus with lower costs without compromising quality.
文摘A systematic experimental study on the self quenching streamer discharge has been performed.The dependence of the charge developed in the discharge on the high voltage can be approximated by a linear relation.The concentration of quenching gas and anode diameter have little influence on the slope of this linear relation.
