Cylasformicarius is one of the most important pests of sweet potato worldwide, causing considerable ecological and economic damage.This study improved the effect of comprehensive management and understanding of geneti...Cylasformicarius is one of the most important pests of sweet potato worldwide, causing considerable ecological and economic damage.This study improved the effect of comprehensive management and understanding of genetic mechanisms by examining the functional genomics of C. formicarius.Using Illumina and PacBio sequencing, this study obtained a chromosome-level genome assembly of adult weevils from lines inbred for 15 generations.The high-quality assembly obtained was 338.84 Mb, with contig and scaffold N50 values of 14.97 and 34.23 Mb, respectively.In total, 157.51 Mb of repeat sequences and 11 907 protein-coding genes were predicted.A total of 337.06 Mb of genomic sequences was located on the 11 chromosomes, accounting for 99.03%of the total length of the associated chromosome.Comparative genomic analysis showed that C. formicarius was sister to Dendroctonus ponderosae, and C. formicarius diverged from D. ponderosae approximately 138.89 million years ago (Mya).Many important gene families expanded in the C. formicarius genome were involved in the detoxification of pesticides, tolerance to cold stress and chemosensory system.To further study the role of odorant-binding proteins (OBPs) in olfactory recognition of C. formicarius, the binding assay results indicated that Cfor OBP4–6 had strong binding affinities for sex pheromones and other ligands.The high-quality C. formicarius genome provides a valuable resource to reveal the molecular ecological basis, genetic mechanism, and evolutionary process of major agricultural pests;it also offers new ideas and new technologies for ecologically sustainable pest control.展开更多
目的探讨免疫检查点抑制剂(ICIs)相关吉兰-巴雷综合征(GBS)的发生规律和临床特点,为临床安全用药提供参考。方法检索中国知网、万方数据库、维普数据库、PubMed、Embase、Web of Science等数据库收录的ICIs相关GBS的个案报道,对其临床...目的探讨免疫检查点抑制剂(ICIs)相关吉兰-巴雷综合征(GBS)的发生规律和临床特点,为临床安全用药提供参考。方法检索中国知网、万方数据库、维普数据库、PubMed、Embase、Web of Science等数据库收录的ICIs相关GBS的个案报道,对其临床特点、神经电生理、脑脊液结果及治疗预后进行分析。检索时间从建库至2021年10月31日。结果纳入文献30篇,涉及患者34例,以老年男性为主,平均年龄为(66±11)岁,年龄范围为37~85岁。中位发病时间为49 d。临床表现以肢体无力、腱反射减退/消失、感觉异常/消失为主;31例脑脊液蛋白不同程度升高。28例(82.35%)患者接受了静注人免疫球蛋白(IVIG)和糖皮质激素的治疗。ICIs相关的GBS预后较差,6例(17.65%)患者基本恢复或完全康复,21例(61.76%)患者治疗后仍遗留持续的神经症状,7例(20.59%)死亡。结论在免疫治疗开始时,应警惕GBS的发生,并做好相关检查和常规监测。一旦怀疑ICIs相关的GBS,应立即给予对症治疗,对于是否重启ICIs治疗需谨慎决定。展开更多
基金supported by the Natural Science Foundation of Guangxi Autonomous Region,China(2022GXNSFAA035558)the Technology Development Foundation of Guangxi Academy of Agricultural Sciences(2021ZX09)+2 种基金the China Agriculture Research System of MOF and MARA(CARS-10-B3 and CARS-10-C19)the Guangxi Innovation Team Construction Project(nycytxgxcxtd-11-03)the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD),China。
文摘Cylasformicarius is one of the most important pests of sweet potato worldwide, causing considerable ecological and economic damage.This study improved the effect of comprehensive management and understanding of genetic mechanisms by examining the functional genomics of C. formicarius.Using Illumina and PacBio sequencing, this study obtained a chromosome-level genome assembly of adult weevils from lines inbred for 15 generations.The high-quality assembly obtained was 338.84 Mb, with contig and scaffold N50 values of 14.97 and 34.23 Mb, respectively.In total, 157.51 Mb of repeat sequences and 11 907 protein-coding genes were predicted.A total of 337.06 Mb of genomic sequences was located on the 11 chromosomes, accounting for 99.03%of the total length of the associated chromosome.Comparative genomic analysis showed that C. formicarius was sister to Dendroctonus ponderosae, and C. formicarius diverged from D. ponderosae approximately 138.89 million years ago (Mya).Many important gene families expanded in the C. formicarius genome were involved in the detoxification of pesticides, tolerance to cold stress and chemosensory system.To further study the role of odorant-binding proteins (OBPs) in olfactory recognition of C. formicarius, the binding assay results indicated that Cfor OBP4–6 had strong binding affinities for sex pheromones and other ligands.The high-quality C. formicarius genome provides a valuable resource to reveal the molecular ecological basis, genetic mechanism, and evolutionary process of major agricultural pests;it also offers new ideas and new technologies for ecologically sustainable pest control.
文摘目的探讨免疫检查点抑制剂(ICIs)相关吉兰-巴雷综合征(GBS)的发生规律和临床特点,为临床安全用药提供参考。方法检索中国知网、万方数据库、维普数据库、PubMed、Embase、Web of Science等数据库收录的ICIs相关GBS的个案报道,对其临床特点、神经电生理、脑脊液结果及治疗预后进行分析。检索时间从建库至2021年10月31日。结果纳入文献30篇,涉及患者34例,以老年男性为主,平均年龄为(66±11)岁,年龄范围为37~85岁。中位发病时间为49 d。临床表现以肢体无力、腱反射减退/消失、感觉异常/消失为主;31例脑脊液蛋白不同程度升高。28例(82.35%)患者接受了静注人免疫球蛋白(IVIG)和糖皮质激素的治疗。ICIs相关的GBS预后较差,6例(17.65%)患者基本恢复或完全康复,21例(61.76%)患者治疗后仍遗留持续的神经症状,7例(20.59%)死亡。结论在免疫治疗开始时,应警惕GBS的发生,并做好相关检查和常规监测。一旦怀疑ICIs相关的GBS,应立即给予对症治疗,对于是否重启ICIs治疗需谨慎决定。
