Influence of varied drought types on soil conservation service within the framework of climate change:insights from the Jinghe River Basin,China

Severe soil erosion and drought are the two main factors affecting the ecological security of the Loess Plateau,China.Investigating the influence of drought on soil conservation service is of great importance to regional environmental protection and sustainable development.However,there is little research on the coupling relationship between them.In this study,focusing on the Jinghe River Basin,China as a case study,we conducted a quantitative evaluation on meteorological,hydrological,and agricultural droughts(represented by the Standardized Precipitation Index(SPI),Standardized Runoff Index(SRI),and Standardized Soil Moisture Index(SSMI),respectively)using the Variable Infiltration Capacity(VIC)model,and quantified the soil conservation service using the Revised Universal Soil Loss Equation(RUSLE)in the historical period(2000-2019)and future period(2026-2060)under two Representative Concentration Pathways(RCPs)(RCP4.5 and RCP8.5).We further examined the influence of the three types of drought on soil conservation service at annual and seasonal scales.The NASA Earth Exchange Global Daily Downscaled Projections(NEX-GDDP)dataset was used to predict and model the hydrometeorological elements in the future period under the RCP4.5 and RCP8.5 scenarios.The results showed that in the historical period,annual-scale meteorological drought exhibited the highest intensity,while seasonal-scale drought was generally weakest in autumn and most severe in summer.Drought intensity of all three types of drought will increase over the next 40 years,with a greater increase under the RCP4.5 scenario than under the RCP8.5 scenario.Furthermore,the intra-annual variation in the drought intensity of the three types of drought becomes smaller under the two future scenarios relative to the historical period(2000-2019).Soil conservation service exhibits a distribution pattern characterized by high levels in the southwest and southeast and lower levels in the north,and this pattern has remained consistent both in the historical and future periods.Over the past 20 years,the intra-annual variation indicated peak soil conservation service in summer and lowest level in winter;the total soil conservation of the Jinghe River Basin displayed an upward trend,with the total soil conservation in 2019 being 1.14 times higher than that in 2000.The most substantial impact on soil conservation service arises from annual-scale meteorological drought,which remains consistent both in the historical and future periods.Additionally,at the seasonal scale,meteorological drought exerts the highest influence on soil conservation service in winter and autumn,particularly under the RCP4.5 and RCP8.5 scenarios.Compared to the historical period,the soil conservation service in the Jinghe River Basin will be significantly more affected by drought in the future period in terms of both the affected area and the magnitude of impact.This study conducted beneficial attempts to evaluate and predict the dynamic characteristics of watershed drought and soil conservation service,as well as the response of soil conservation service to different types of drought.Clarifying the interrelationship between the two is the foundation for achieving sustainable development in a relatively arid and severely eroded area such as the Jinghe River Basin.

益胃解毒方治疗慢性萎缩性胃炎的效果及对Cyclin E、Th17/Treg比值的影响分析

目的分析益胃解毒方治疗慢性萎缩性胃炎中的效果及对细胞周期蛋白E(Cyclin E)水平、辅助性T细胞(Th17)/调节性T细胞(Treg)比值的影响。方法选取2019年6月至2022年6月在河北省石家庄市中医院确诊的150例慢性萎缩性胃炎患者作为研究对象,按照随机数字表法分为益胃解毒方组、六君子汤组、益胃解毒方+六君子汤组,每组50例。益胃解毒方组使用益胃解毒方治疗,六君子汤组使用六君子汤治疗,益胃解毒方+六君子汤组使用益胃解毒方联合六君子汤治疗,均连续治疗24周。比较3组治疗效果、中医证候积分、Cyclin E水平、Th17/Treg比值、不良反应发生情况。结果益胃解毒方组治疗总有效率高于六君子汤组和益胃解毒方+六君子汤组,且益胃解毒方+六君子汤组高于六君子汤组,差异均有统计学意义(P<0.05)。治疗后3组各项中医证候积分低于治疗前,差异均有统计学意义(P<0.05)。治疗后益胃解毒方组各项中医证候积分低于六君子汤组和益胃解毒方+六君子汤组,且益胃解毒方+六君子汤组低于六君子汤组,差异均有统计学意义(P<0.05)。治疗后3组Cyclin E水平、Th17/Treg比值均低于治疗前,差异均有统计学意义(P<0.05)。治疗后益胃解毒方组Cyclin E水平、Th17/Treg比值低于六君子汤组和益胃解毒方+六君子汤组,且益胃解毒方+六君子汤组低于六君子汤组,差异均有统计学意义(P<0.05)。益胃解毒方组总不良反应发生率低于六君子汤组和益胃解毒方+六君子汤组,且益胃解毒方+六君子汤组低于六君子汤组,差异均有统计学意义(P<0.05)。结论益胃解毒方治疗慢性萎缩性胃炎效果显著,不仅能够改善患者临床症状,调节Cyclin E、Th17/Treg表达,还能减少不良反应发生,值得临床推广应用。

甲基三甲氧基硅烷对Li/NCM811电池性能提升的影响

LiNi_(0.8)Co_(0.1)Mn_(0.1)O_(2)(NCM811)是一种高镍锂电池的正极材料,比容量高,但在应用中存在阳离子混排、表面结构不稳定等问题。采用金属锂片为负极,1 mol/L LiPF_(6)-EC∶DEC(体积比为1∶1)为电解液,研究了甲基三甲氧基硅烷(MTMS)作电解液添加剂对Li/NCM811电池电化学性能及正极表面性质的影响。研究发现,在电解液中加入质量分数4%的MTMS,0.5 C的电流下充放电循环100圈后,电池容量保持率增加131.95%。电化学测试及反应前后电极表面性质表征结果表明,MTMS添加剂有助于NCM811表面形成稳定致密的CEI膜,抑制电解液分解,有效保护了活性位点的化学稳定性,抑制了电极材料不可逆相变的进行。

准格尔煤田老三沟外围勘查区煤系Al-Ga-Li-Nb(Ta)-Zr(Hf)共富集成因及资源潜力评价

内蒙古准格尔煤田石炭纪—二叠纪煤系蕴含丰富的铝、镓、锂及稀土等多种战略性金属。为明确该煤系Al-Ga-Li-Nb(Ta)-Zr(Hf)等战略性金属富集的成因机制,本文以准格尔煤田老三沟外围勘查区典型钻孔石炭纪—二叠纪煤系为研究对象,采用X射线衍射分析(XRD)、X射线荧光光谱仪(XRF)、电感耦合等离子体质谱仪(ICP-MS)、带能谱的扫描电镜和基于Tescan Mira扫描电镜的TIMA-X综合矿物分析仪等系统分析了煤的矿物学和元素地球化学特征。研究区太原组和山西组煤系中普遍存在Al-Li-Ga以及Nb-Ta-Zr-Hf的共富集,其中太原组煤系主可采6号煤中Li、Ga、Zr以及局部层位Nb、Ta均达工业利用品位,具有重要的成矿潜力和开发利用价值。煤中富集的Li和Ga主要赋存于高岭石、勃姆石中,部分Li还赋存于绿泥石中。煤中Al-Ga-Li-Nb(Ta)-Zr(Hf)的富集受控于陆源区母岩的化学风化、火山碎屑的输入、流体活动以及成岩过程中复杂的水/岩作用过程。华北地台前石炭系沉积基底和阴山古陆中元古代钾长花岗岩长期的强烈风化淋滤,形成富Ga-Li的铝土质古风化壳残积物,为研究区煤中铝、镓、锂的初步富集提供了物质来源。富集的铝、镓、锂随地下水等流体迁移,在水动力相对较弱的潟湖、泥炭沼泽等水体沉淀富集。在泥炭化和成岩阶段,富集Li、Ga、Nb、Ta等战略性金属的中性—长英质碱性火山灰的输入以及地下水、低温热液、酸性流体的迁移导致了煤系Li、Ga等战略性金属的二次富集。

Recent Progress of the Solar Wind Magnetosphere Ionosphere Link Explorer (SMILE) Mission

The SMILE(Solar wind Magnetosphere Ionosphere Link Explorer)mission is a joint space science mission between the Chinese Academy of Sciences(CAS)and the European Space Agency(ESA),aiming to understand the interaction of the solar wind with the Earth’s magnetosphere in a global manner.As of May 2024,the SMILE mission is in phase-D with an expected launch date of September 2025.This report summarizes developments in the mission during the past two years.

Protective effect of camellia oil on H_(2)O_(2)-induced oxidative stress injury in H9C2 cardiomyocytes of rats

Objective: To explore the protective effect of camellia oil against H2O2-induced oxidative stress injury in rat H9C2 cardiomyocytes. Methods: CCK8 method was used to detect the cell survival rate of H9C2 cardiomyocytes treated with different concentrations of H2O2. Normal cultured cells were used as the blank control group, and the cells were treated with 200 μmol/L H2O2 for 24 h. An oxidative stress injury model was constructed as the model group. The cells were pretreated with 1%, 0.1% and 0.01% camellia oil for 24 h, and then H2O2 was added for 24 h as the experimental group. The β-galactosidase senescence staining assay, mitochondrial membrane potential assay, EdU cell proliferation staining assay and scratch assay were used to observe the changes of cell senescence, mitochondrial membrane potential, proliferation, apoptosis and migration in each group. The superoxide dismutase (SOD) activity, lactate dehydrogenase (LDH) activity, and malondialdehyde (MDA) content of the cells in each group were detected by using the kit. Results: The cell viability of H9C2 cardiomyocytes treated with different concentrations of H2O2 was inhibited and positively correlated with the concentration of H2O2 (P<0.01). Compared with the blank control group, the positive rate of cell senescence, MDA content and LDH activity increased in the H2O2 model group (P<0.01);mitochondrial membrane potential, cellular value-added rate, migration rate and SOD activity decreased (P<0.01). Compared with the H2O2 model group, the positive rate of cellular senescence (P<0.01 or P<0.05), MDA content and LDH activity decreased (P< 0.01 or P<0.05);mitochondrial membrane potential increased, cell proliferation rate and migration rate increased (P<0.01 or P<0.05) in the experimental group. Conclusion: Camellia oil can significantly inhibit oxidative stress injury in H9C2 cells and exert cardiomyocyte protective effects.

Exploring Paths for the High-Quality Development of Forest-Based Wel ness Industry:A Case Study of Sichuan Province

The forest-based wellness industry,as a rapidly growing sector that integrates various business forms with extensive coverage and an extended industrial chain,is undergoing rapid development due to the increasing aging population and people's suboptimal health conditions.As a forerunner in developing the forest-based wellness industry,Sichuan province is known for its early development,proactive efforts,diverse models,and significant impact in this industry.It has achieved certain milestones in terms of top-level design,pilot demonstration,standardized guidance,and public awareness campaigns to promote the development of this industry.Therefore,this paper utilizes Sichuan as a case study to systematically summarize and analyze the key practices made by the province in promoting the rapid development of the industry by investigating the development trajectory of the forest-based wellness industry.Additionally,it examines the development trends of this industry from the perspectives of supply,demand,and consumption.Finally,this paper proposes several measures to facilitate the high-quality development of the forest-based wellness industry.These measures encompass nurturing specialized talent in forest-based wellness,enhancing market players'capabilities in this domain,conducting extensive research on technologies that promote this industry,actively seeking support from relevant policies,and promoting integrated development across diverse sectors.

The status evaluation of the permafrost environment along the Chaida'er-Muli Railway in southern Qilian Mountains in northern Qinghai Province,China

Engineering construction has major influence on the permafrost environment.This paper analyzes the interaction between engineering construction and permafrost environment along the Chaidaer-Muli Railway(simply,CMR) based on the press-state-response(PSR) framework.The permafrost environmental system is divided into three subsystems,consisting of permafrost thermal stability,proneness to the freeze-thawing erosion and permafrost ecological fragility.Each subsystem considers its most important influencing factors.Catastrophe Progression Method(CPM) is applied to calculate the current environment condition along the railway.The result indicates that:(1) as far as the thermal stability is concerned,most sections along the CMR are mainly concentrated in rank Ⅲ(fair situation),and a few in Ⅱ(good situation) and Ⅳ(bad situation),respectively;(2) for the proneness tothe freeze-thawing erosion,the entire railway route falls largely in rank Ⅱ(good situation);(3) along the CMR,the ecological fragility of the permafrost environment is in rank Ⅱ(good situation),or slightly fragile;(4) overall,the permafrost environments along the CMR are in rank Ⅲ(fair situation) or Ⅱcondition(good situation).In general,the permafrost environment along the CMR is fair.It is mainly because a series of active measures of protecting permafrost were taken for stabilizing the CMR foundation soils.On the one hand,we should try our best to minimize the influences that engineering activities have exerted on ecology and environment,on the other hand,the positive measures have made improvements to prevent the permafrost environment from deterioration.

环境因素对Bacillus amyloliquefaciens TF28在土壤中定殖的影响

为了明确生防菌株 Bacillus amyloliquefaciens TF28在土壤中的定殖动态,采用活菌计数及菌株特异PCR验证相结合的方法,研究温度、含水量、pH、接种量、土壤类型和病原菌对其在离体土壤中定殖的影响。结果表明,适于菌株TF28定殖的温度、pH、含水量范围较广,在20~35 ℃,pH 4.5~8.6,含水量 5%~35%范围内均能有效定殖;25 ℃、弱酸性和中性、15%含水量更利于其定殖;接种量和病原菌显著影响其定殖,接种量越大,定殖能力越强;病原菌在接种后14 d内显著促进其定殖;另外,土壤有机质质量分数高更利于其定殖。该研究为菌株TF28的推广应用提供了理论依据。

Beclin-1基因shRNA慢病毒载体的构建及其对B16F10细胞自噬及活力的影响

利用RNA干扰技术构建稳定沉默Beclin-l基因的B16F10小鼠黑色素瘤细胞系,分析稳转细胞系的自噬水平和活力,为探讨Beclin-1基因、自噬与肿瘤三者关系奠定基础。构建小鼠Beclin-l基因干扰载体,转染至293T细胞,获得慢病毒颗粒,将病毒颗粒感染B16F10细胞,通过加压筛选、有限稀释、细胞驯化得到稳定转染m-Beclin-1-shRNA1的B16F10细胞系。采用RT-PCR和免疫荧光技术检测对Beclin-l的干扰效果,Western blot和透射电镜分析稳转细胞系中自噬标志物LC3蛋白表达及自噬小体形成情况,CCK-8法检测稳转细胞系的活力。结果表明,成功构建了靶向抑制Beclin-l基因表达的shRNA,纯化的慢病毒滴度为1×10^(8) TU·mL^(-1),建立的细胞系中Beclin-1 mRNA和蛋白表达都受到不同程度的抑制,mRNA的沉默效率约为75%(P<0.01),发现干预Beclin-1表达能够抑制LC3-Ⅱ蛋白的表达及自噬小体的形成数量,降低B16F10细胞活力。以上结果表明,干扰Beclin-1表达能够有效降低B16F10细胞自噬活性,促进B16F10细胞死亡,这为探讨Beclin-1基因功能及自噬在抗肿瘤中的作用奠定重要基础。

基于Simulink的磷酸铁锂电池内阻在线监测与故障诊断

随着新能源汽车的快速发展,磷酸铁锂电池作为其核心能量,其性能监测与故障诊断对保障新能源汽车的顺畅运行有不可替代的作用。该文在观点论述的过程中,针对磷酸铁锂电池内阻的诊断提出了一种基于Simulink的磷酸铁锂电池内阻在线监测与故障诊断方法。在研究过程中,针对文中提出的内阻监测的基本原理进行了简单的说明,并构建了基于Simulink的内阻监测模型,还介绍了故障诊断的基本原理和基于数据分析的故障诊断方法。为印证文中给出方案的准确性,在最后通过实际案例,验证了Simulink在磷酸铁锂电池内阻监测与故障诊断中的有效性,希望为磷酸铁锂电池的安全运行和维护提供有力支持。

LIF和CNN的矿井突水水源类型判别

矿井进入深部开采过程中,突水威胁分别来自顶板老空水和底板高压岩溶水。煤矿突水水源类型的在线识别能够预警煤矿水害,是矿井水害防治关键环节,对煤矿安全生产具有积极意义。代表离子法作为传统的煤矿突水水源类型识别方法,需要深入现场采集水样,密封处理后在实验室检测水样中7种典型的无机离子浓度,计算得到突水评价因子。这种存在检测周期过长、样品易被污染以及预警响应滞后、无法在线判别等不利因素。针对代表离子法方法的不足,提出了一种基于激光诱导荧光(LIF)和卷积神经网络(CNN)的矿井突水水源判别模型。首先,针对淮南矿业集团新集二矿的4种水体, 2016年6月—2017年6月期间分批次取得161组水源样本,其中采空区积水46条,砂岩水59条,太灰水42条和奥灰水14条。用LIFS-405激光诱导荧光系统发射的405 nm激光检测水样,水体受激后得到突水水样的荧光光谱。主成分分析得到前10个主成分累计贡献率不足85%, 4种水样无法有效直接辨识。针对该问题和水样荧光光谱中的随机高频波动干扰,采用一阶滞后滤波方法抑制波动频率较高的周期性干扰;针对线判别分析对数据更新率的要求,采用递推平均方法;在此基础上,提出了一种改进的递推平均一阶滞后平滑滤波方法,并对滤波处理后的荧光光谱进行自相关计算,得到二维自相关荧光光谱特征图。实验表明,采用改进后的滤波法处理方法,计算得到的4种测试水样的二维荧光光谱图较好的滤除了噪声干扰,并表现了出了明显的差异性。针对二维自相关荧光光谱特征图,构建了基于卷积神经网络(CNN)的突水水源类型判别模型,用于判别突水水源类型。该方法采用深度学习的模型框架,直接对二维自相关荧光光谱特征图进行识别,有效避免了PCA降维的片面性。理论分析和实验结果表明:该模型对水源类型的准确识别率达到了98%,是一种有效的矿井突水水源类型判别方法,为在线矿井突水水源类型判别方法提供了新的思路。

分段Filippov系统的簇发振荡及擦边运动机理

本文旨在揭示非光滑Filippov系统中由频域上不同尺度耦合导致的簇发振荡行为及其产生机理.以经典的周期激励Duffing振子为例,通过引入对状态变量的分段控制及适当选取参数,使得激励频率与系统固有频率之间存在量级差距,建立了频域两尺度耦合的Filippov系统.当激励频率远小于系统的固有频率时,可以将整个激励项视为慢变参数或慢变子系统,从而得到广义自治快子系统.分析了由非光滑分界面划分的不同区域中各快子系统的平衡点及其分岔特性随慢变参数变化的演化过程.考察了两种典型参数条件下系统的振荡行为及其动力学特性,指出参数变化不仅会引起其相应子系统平衡曲线及其分岔特性的改变,也会导致不同模式的簇发振荡.同时,轨迹穿越非光滑分界面时会产生不同的动力学行为,特别是在一定参数条件下,由于运动轨迹受不同子系统的交替控制,存在着擦边运动现象,从而导致特殊形式的非光滑簇发振荡.基于转换相图及各区域中快子系统的平衡曲线及其分岔特性,揭示了非光滑分界面对系统簇发振荡的影响规律及不同簇发振荡的分岔机理.

基于3DE平台Library架构的模板管理方法研究

在使用3DE平台的设计工作中,随着标准化模板积累,特别是抽水蓄能电站中,水工建筑物结构形式较为统一,大量采用了标准化模板建模,客观上导致模板在创建、发布、升级、拓展、归集、查询、应用等环节出现问题,影响设计效率。针对上述问题,开展了Library架构的3DE模板系统化管理方法的研究,探索了该架构下模板库创建、查询、应用的技术路线,并完成了抽水蓄能电站水工结构模板库的搭建。

ELISA抗体检测试剂盒与病毒中和试验对临床牛血清样本中牛结节性皮肤病病毒抗体的检测比对

病毒中和试验是血清学检测的金标准。为了评估中牧实业股份有限公司试制的牛结节性皮肤病病毒ELISA抗体检测试剂盒的检测效果,本研究用3批牛结节性皮肤病病毒ELISA抗体检测试剂盒与病毒中和试验同时对260份牛结节性皮肤病病毒(LSDV)感染牛血清和200份健康牛血清进行比对检测。结果显示,中牧实业股份有限公司试制的牛结节性皮肤病病毒ELISA试剂盒阳性检出率为86.9%(226/260),病毒中和试验阳性检出率为81.9%(213/260),二者对260份LSDV感染牛血清的检测符合率为85.8%(223/260),对200份健康牛血清的检测符合率为100%(200/200)。结果表明,中牧实业股份有限公司试制的牛结节性皮肤病病毒ELISA抗体检测试剂盒敏感性高、特异性好、检测结果准确度较高。

应用LSDV-ELISA抗体检测试剂盒检测羊痘疫苗免疫牛血清抗体水平

目前国内尚无商品化的牛结节性皮肤病疫苗,对于该病的防控,我国采用羊痘疫苗进行紧急免疫。为了验证牛结节性皮肤病病毒ELISA抗体检测试剂盒对羊痘疫苗免疫血清抗体水平检测的适用性,本研究采用实验室试制的牛结节性皮肤病病毒ELISA抗体检测试剂盒和病毒中和试验对羊痘病毒活疫苗免疫血清、在研牛结节性皮肤病灭活疫苗(山羊痘AV41株)免疫血清进行抗体水平评价。结果显示,对于羊痘活疫苗免疫血清,ELISA方法和病毒中和试验方法对免疫15d抗体阳性率分别为84.4%和75.6%,免疫30d抗体阳性率分别为100%和95.6%,免疫60d抗体阳性率分别为97.8%和91.1%;与病毒中和试验符合率为86.1%。对于牛结节性皮肤病灭活疫苗(山羊痘AV41株)免疫血清,ELISA方法和病毒中和试验方法对一免28d抗体阳性率分别为64%和56%,二免28d抗体阳性率分别为94%和88%,二免42d阳性率均为100%;与病毒中和试验符合率为86.5%。试验证明,实验室试制的牛结节性皮肤病病毒ELISA抗体检测试剂盒与病毒中和试验符合率较高,检测结果较为一致,且本试剂盒敏感性高于病毒中和试验。因此本试剂盒可用于牛结节性皮肤病抗体水平检测。

2019年新疆部分地区猪伪狂犬病毒gB、gE蛋白抗体ELISA检测及分析

为调查2019年新疆部分地区猪群伪狂犬病毒(pseudorabies virus,PRV)免疫抗体水平和野毒感染情况,试验应用猪伪狂犬病毒gB、gE抗体检测试剂盒对采集的8个规模化猪场441份血清样品进行PRV-gB和PRV-gE两种蛋白的抗体检测。结果显示:8个规模化猪场PRV-gB平均抗体阳性检出率为90.93%,其中B、C、F场平均抗体阳性检出率均为100.00%,A场平均抗体阳性检出率最低,为16.00%。8个规模化猪场PRV-gE平均抗体阳性检出率为44.67%,其中B场平均阳性检出率最高,为92.47%。A场和D场平均阳性检出率最低,为0。对不同生长阶段的猪群分析,PRV-gB抗体阳性检出率最高为种公猪95.95%,最低为保育猪75.00%;PRV-gE抗体阳性检出率最高为后备母猪78.95%,最低为育肥猪11.43%。试验表明,新疆部分地区猪群存在野毒感染,各养猪场之间、不同生长阶段感染情况不一。该试验可以为新疆部分地区猪场PR防控与净化提供参考。

种芽生物营养强化机的设计与试验

针对营养功能性农产品供给不足和生物营养强化技术装备落后等问题,设计了种芽生物营养强化机。采取提高组分浓度差、压力差,以及透水交变真空等快速催芽技术,解决营养组分向种芽内高效传质问题。经绿豆芽富叶酸营养强化试验和发芽糙米富钙营养强化试验研究,结果表明:种芽生物营养强化机可生产高营养价值农产品,其生产的绿豆芽中叶酸含量可达1.486 mg/kg,发芽糙米中钙含量可达757 mg/kg,营养强化效果良好。

微波消融治疗Klippel-Trenaunay综合征患儿边缘静脉的效果和安全性

目的探讨微波消融术治疗Klippel-Trenaunay综合征(KTS)患儿边缘静脉(LMV)的效果和安全性。方法回顾性分析2021年1月至2021年6月在山东大学附属儿童医院接受血管内微波消融闭合术治疗LMV的8例KTS患儿临床资料。统计患儿一般资料、临床疗效及术后不良反应。结果8例患儿中男女各4例,首次就诊时中位年龄为6岁(9个月~17岁),其中患肢为左下肢5例,右下肢3例。4例患儿接受LMV主干全程消融,3例为大腿段LMV消融,1例为小腿段LMV消融。术后即刻造影显示7例完全闭合,1例闭合不全,继续泡沫硬化剂注射治疗。中位随访3.9个月(2~6个月),有1例再通,表现为串珠样改变,再次消融后闭合。所有患儿术后出现不同程度的肢体肿胀,1例发生血栓性静脉炎,1例术后一过性发热。结论血管内微波消融治疗KTS患儿LMV安全有效,并发症发生率低,患儿耐受性好。

Apelin基因rs2235306多态性与2型糖尿病早期肾损害的关系

目的探讨Apelin基因rs2235306多态性与2型糖尿病早期肾损害的关系。方法选取121例2型糖尿病患者,根据尿清蛋白与肌酐的比值分为正常清蛋白尿组(NA组,58例)和微量清蛋白尿组(MA组,63例);选择健康志愿者60名为健康对照组(NC组)。应用四引物扩增受阻突变体系聚合酶链反应(ARMS-PCR)技术,测定上述三组人群Apelin基因rs2235306多态性,同时测定血清Apelin水平及生化指标。结果 (1) NC组三种基因型频率分别为TT 43%、TC 45%、CC 12%,等位基因频率为T 66%、C 34%; NA组相应为TT 40%、TC 45%、CC 15%,T 62%、C 38%; MA组相应为TT 33%、TC 40%、CC 27%,T 53%、C 47%。MA组CC基因型频率及C等位基因频率明显高于NC组(χ2分别为4. 591、4. 082,P值为0. 032、0. 043); NA组CC基因型频率及C等位基因频率与NC组比较,差异无统计学意义。(2)糖尿病人群TC+CC基因型组与TT基因型组比较,空腹胰岛素(FIns)、胰岛素抵抗指数(HOMA-IR)和Apelin水平明显升高(均P <0. 05)。(3)多因素回归分析结果显示,Apelin与HOMA-IR和BMI正相关(t=4. 078和2. 071,均P <0. 05)。结论 Apelin基因rs2235306位点C等位基因携带可能与2型糖尿病早期肾损害有关。