The identification and localization of vertebrate follicle stimulating hormone （FSH） and luteinizing hormone （LH） in crustaceans may further elucidate the regulation mechanisms in arthropod repro-duction. Using im...The identification and localization of vertebrate follicle stimulating hormone （FSH） and luteinizing hormone （LH） in crustaceans may further elucidate the regulation mechanisms in arthropod repro-duction. Using immunocytochemical staining techniques, this study has localized vertebrate FSH-like and LH-like substances in neurons in the subesophageal and thoracic ganglia from the thoracic ganglion mass （TGM） of Scylla paramamosain （Crustacea： Decapoda： Brachyura）. Enzyme-linked immunosorbent assay （ELISA） has shown that the concentrations of both FSH-and LH-like sub-stances increased markedly in the TGM during the vitellogenic stage compared with that in the previtellogenic stage. These results indicate that substances resembling the vertebrate FSH and LH are present in S. paramamosain, and they may be involved in the development of the ovary as well as in ovulation.展开更多
The temporal and spatial variabilities of phytoplankton absorption coefficients （a ph （λ）） and their relationships with physical processes in the northern South China Sea were examined, based on in situ data coll...The temporal and spatial variabilities of phytoplankton absorption coefficients （a ph （λ）） and their relationships with physical processes in the northern South China Sea were examined, based on in situ data collected from two cruise surveys during May 14 to 25, 2001 and November 2 to 21, 2002. Significant changes in the surface water in a ph values and B/R ratios （a ph （440）/a ph （675）） were observed in May, which were caused by a phytoplankton bloom on the inner shelf stimulated by a large river plume due to heavy precipitation. This is consistent with the observed one order of magnitude elevation of chlorophyll a and a shift from a pico/nano dominated phytoplankton community to one dominated by micro-algae. Enhanced vertical mixing due to strengthened northeast monsoon in November has been observed to result in higher surface a ph （675） （0.002–0.006 m-1 higher） and less pronounced subsurface maximum on the outer shelf/slope in November as compared with that in May. Measurements of a ph and B/R ratios from three transects in November revealed a highest surface a ph （675） immediately outside the mouth of the Zhujiang （Pearl） River Estuary, whereas lower a ph （675） and higher B/R ratios were featured in the outer shelf/slope waters, demonstrating the respective influence of the Zhujiang River plume and the oligotrophic water of the South China Sea. The difference in spectral shapes of phytoplankton absorption （measured by B/R ratios and bathochromic shifts） on these three transects infers that picoprocaryotes are the major component of the phytoplankton community on the outer shelf/slope rather than on the inner shelf. A regional tuning of the phytoplankton absorption spectral model （Carder et al., 1999） was attempted, demonstrating a greater spatial variation than temporal variation in the lead parameter a 0 （λ）. It was thus implicated that region-based parameterization of ocean color remote sensing algorithms in the northern South China Sea was mandatory.展开更多
The horizontal and vertical distribution patterns of five planktonic copepods, Calanus sinicus, Acartia pacifica, Tortanus derjugini, Acartiella sinensis and Pseudodiaptomus poplesia, predominant in the Jiulong Estuar...The horizontal and vertical distribution patterns of five planktonic copepods, Calanus sinicus, Acartia pacifica, Tortanus derjugini, Acartiella sinensis and Pseudodiaptomus poplesia, predominant in the Jiulong Estuary, were investigated from May 2003 to April 2004. The results showed that the distribution of these copepods was related to the tidal period but that each species had its own specific pattern. C. sinicus showed no tidal vertical migration behavior and was thought to be a non-resident species in this estuary. Among Acartia pacifica, T. derjugini,Acartiella sinensis, more individuals occurred in the surface than in the bottom waters during flood tide, and the pattern was reversed during ebb tide. The epibenthic copepod P. poplesia usually remained in the bottom waters in the upstream part of the estuary, but it displayed strong tidally-oriented vertical migration in the middle reaches of the estuary. Taking into account the hydrographic characteristics of the Jiulong Estuary, it was hypothesized that the planktonic copepods in this estuary had more or less adopted the mechanism of vertically migrating to the surface waters during flood tide in order to make use of the inflowing tide, and then sinking to the bottom during ebb tide to avoid being carried out of the estuary by net outflow.展开更多
The seasonal occurrence of Acartia pacifica （ Copepoda： Calanoida） and their resting eggs in the sediment of Xiamen Bay were documented between October 2002 and September 2003. The numher of viable eggs in the sedi...The seasonal occurrence of Acartia pacifica （ Copepoda： Calanoida） and their resting eggs in the sediment of Xiamen Bay were documented between October 2002 and September 2003. The numher of viable eggs in the sediment increased from January to May with the increase in the numher of planktonic females. When the population ofA. pacifica disappeared from the water cohinm, the number of eggs in the sediment began to decrease and reached a low value due to lack of input. The peak of nauplii abundance occurred when the hatching potential of eggs from the sediment was high under the natural environment from February to June. The hatching of resting eggs of A. pacifica was essentially temperature-dependent after suspension, while photoperied regimes had no significant effect on the hatching. The mean density of subitaneeus eggs was 1. 122 0 g/cm^3 with a standard deviation （SD） of 0. 000 2 g/cm^3. The mean density of diapause eggs was 1. 151 2 g/cm^3 with a SD of 0.000 1 g/cm^3. The sinking rates of subitaneons eggs ranged from 19.55 to 26.17 m/d, while those of diapause eggs ranged from 30.29 to 31.28 m/d. The comparison of the egg deposition time and egg hatching time suggested that in most cases virtually all subitaneous eggs of A. pacifica would settle to the bottom before their hatching even though the eggs have high potential to hatch. The evidence was provided that the seasonal dynamics of A. pacifica is accompanied by benthic-pelagic coupling.展开更多
Ecological experiments were conducted to examine the effects of seawater containing elevated par- tial pressure of carbon dioxide （p CO2 800 × 10 -6 , 2 000 × 10 -6 , 5 000 × 10 -6 and 10 000 × 10...Ecological experiments were conducted to examine the effects of seawater containing elevated par- tial pressure of carbon dioxide （p CO2 800 × 10 -6 , 2 000 × 10 -6 , 5 000 × 10 -6 and 10 000 × 10 -6 ） on the survival and reproduction of female Acartia pacifica, Acartia spinicauda, Calanus sinicus and Centropages tenuiremis, which are the dominant copepods in the southern coastal waters of China. The results show that the effects of elevated p CO2 on the survival rates of copepods were species-specific. C. sinicus, which was a macro-copepod, had a higher survival rate （62.01%–71.96%） than the other three species （5.00%–26.67%） during the eight day exposure. The egg production rates of C. sinicus, A. spinicauda and C. tenuiremis were significantly inhibited by the increased p CO2 and the exposure time duration. There were significantly negative impacts on the egg hatching success of A. spinicauda and C. tenuiremis in the p CO2 2 000 × 10 -6 and 10 000 × 10 -6 groups, and, in addition, the exposure time had noticeably impacts on these rates too. This study indicates that the reproductive performances of copepods were sensitive to elevated p CO2 , and that the response of different copepod species to acidified seawater was different. Furthermore, the synergistic effects of seawater acidification and climate change or other pollutant stresses on organisms should be given more attention.展开更多
The larvae of ascidian Styela canopus Savigny were treated with epinephrine, norepinephrine, L-DOPA, GABA and thyroxin to test the ability of these compounds to induce or inhibit larval settlement and metamorphosis. T...The larvae of ascidian Styela canopus Savigny were treated with epinephrine, norepinephrine, L-DOPA, GABA and thyroxin to test the ability of these compounds to induce or inhibit larval settlement and metamorphosis. The results showed that epinephrine, norepinephrine and L-DOPA at the concentration of 1 μmol/dm^3 induced larval settlement and metamorphosis in S. canopus, with short exposure （ 1 h） to 1 μmol/dm^3 of L-DOPA inducing rapid settlement. In contrast, GABA at the concentrations of 0.1 ～1130.0 μmol/dm^3 significantly inhibited the settlement and metamorphosis of S. canopus larvae. In addition, thyroxin at 1 -50 μg/dm^3 had no effect on larval settlement and metamorphosis in S. canopus. These results suggest the importance of neurotransmitters in the settlement and metamorphosis of S. canopus larvae.展开更多
基金The National Natural Science Foundation of China under contract Nos 40406030 and 40776084the National High Technology Research and Development Program of China under contract No 2006AA10A406the Program for New Century Excellent Talents in Fujian Province University
文摘The identification and localization of vertebrate follicle stimulating hormone （FSH） and luteinizing hormone （LH） in crustaceans may further elucidate the regulation mechanisms in arthropod repro-duction. Using immunocytochemical staining techniques, this study has localized vertebrate FSH-like and LH-like substances in neurons in the subesophageal and thoracic ganglia from the thoracic ganglion mass （TGM） of Scylla paramamosain （Crustacea： Decapoda： Brachyura）. Enzyme-linked immunosorbent assay （ELISA） has shown that the concentrations of both FSH-and LH-like sub-stances increased markedly in the TGM during the vitellogenic stage compared with that in the previtellogenic stage. These results indicate that substances resembling the vertebrate FSH and LH are present in S. paramamosain, and they may be involved in the development of the ovary as well as in ovulation.
基金The National Basic Research Program of China under contract Nos 2009CB421200, 2009CB421201the National Natural Science Foundation of China under contract No40821063High-Tech R&D Program of China under contract Nos2006AA09A302 and 2008AA09Z108
文摘The temporal and spatial variabilities of phytoplankton absorption coefficients （a ph （λ）） and their relationships with physical processes in the northern South China Sea were examined, based on in situ data collected from two cruise surveys during May 14 to 25, 2001 and November 2 to 21, 2002. Significant changes in the surface water in a ph values and B/R ratios （a ph （440）/a ph （675）） were observed in May, which were caused by a phytoplankton bloom on the inner shelf stimulated by a large river plume due to heavy precipitation. This is consistent with the observed one order of magnitude elevation of chlorophyll a and a shift from a pico/nano dominated phytoplankton community to one dominated by micro-algae. Enhanced vertical mixing due to strengthened northeast monsoon in November has been observed to result in higher surface a ph （675） （0.002–0.006 m-1 higher） and less pronounced subsurface maximum on the outer shelf/slope in November as compared with that in May. Measurements of a ph and B/R ratios from three transects in November revealed a highest surface a ph （675） immediately outside the mouth of the Zhujiang （Pearl） River Estuary, whereas lower a ph （675） and higher B/R ratios were featured in the outer shelf/slope waters, demonstrating the respective influence of the Zhujiang River plume and the oligotrophic water of the South China Sea. The difference in spectral shapes of phytoplankton absorption （measured by B/R ratios and bathochromic shifts） on these three transects infers that picoprocaryotes are the major component of the phytoplankton community on the outer shelf/slope rather than on the inner shelf. A regional tuning of the phytoplankton absorption spectral model （Carder et al., 1999） was attempted, demonstrating a greater spatial variation than temporal variation in the lead parameter a 0 （λ）. It was thus implicated that region-based parameterization of ocean color remote sensing algorithms in the northern South China Sea was mandatory.
基金the National Natural Science Foundation of China under contract No. 40576065.
文摘The horizontal and vertical distribution patterns of five planktonic copepods, Calanus sinicus, Acartia pacifica, Tortanus derjugini, Acartiella sinensis and Pseudodiaptomus poplesia, predominant in the Jiulong Estuary, were investigated from May 2003 to April 2004. The results showed that the distribution of these copepods was related to the tidal period but that each species had its own specific pattern. C. sinicus showed no tidal vertical migration behavior and was thought to be a non-resident species in this estuary. Among Acartia pacifica, T. derjugini,Acartiella sinensis, more individuals occurred in the surface than in the bottom waters during flood tide, and the pattern was reversed during ebb tide. The epibenthic copepod P. poplesia usually remained in the bottom waters in the upstream part of the estuary, but it displayed strong tidally-oriented vertical migration in the middle reaches of the estuary. Taking into account the hydrographic characteristics of the Jiulong Estuary, it was hypothesized that the planktonic copepods in this estuary had more or less adopted the mechanism of vertically migrating to the surface waters during flood tide in order to make use of the inflowing tide, and then sinking to the bottom during ebb tide to avoid being carried out of the estuary by net outflow.
基金We appreciate the support from the National Natural Science Foundation of China under contract Nos 40506002 and 40076034the State 0ceanic Administration Foundation for Young Scientists of China under contract No.2006119the Polar Research Institute of China Innovation Foundation of Polar Science for Young Scientists of China under contract No.JDQ200502.
文摘The seasonal occurrence of Acartia pacifica （ Copepoda： Calanoida） and their resting eggs in the sediment of Xiamen Bay were documented between October 2002 and September 2003. The numher of viable eggs in the sediment increased from January to May with the increase in the numher of planktonic females. When the population ofA. pacifica disappeared from the water cohinm, the number of eggs in the sediment began to decrease and reached a low value due to lack of input. The peak of nauplii abundance occurred when the hatching potential of eggs from the sediment was high under the natural environment from February to June. The hatching of resting eggs of A. pacifica was essentially temperature-dependent after suspension, while photoperied regimes had no significant effect on the hatching. The mean density of subitaneeus eggs was 1. 122 0 g/cm^3 with a standard deviation （SD） of 0. 000 2 g/cm^3. The mean density of diapause eggs was 1. 151 2 g/cm^3 with a SD of 0.000 1 g/cm^3. The sinking rates of subitaneons eggs ranged from 19.55 to 26.17 m/d, while those of diapause eggs ranged from 30.29 to 31.28 m/d. The comparison of the egg deposition time and egg hatching time suggested that in most cases virtually all subitaneous eggs of A. pacifica would settle to the bottom before their hatching even though the eggs have high potential to hatch. The evidence was provided that the seasonal dynamics of A. pacifica is accompanied by benthic-pelagic coupling.
基金The State Oceanic Administration Foundation of China under contract No.200805029
文摘Ecological experiments were conducted to examine the effects of seawater containing elevated par- tial pressure of carbon dioxide （p CO2 800 × 10 -6 , 2 000 × 10 -6 , 5 000 × 10 -6 and 10 000 × 10 -6 ） on the survival and reproduction of female Acartia pacifica, Acartia spinicauda, Calanus sinicus and Centropages tenuiremis, which are the dominant copepods in the southern coastal waters of China. The results show that the effects of elevated p CO2 on the survival rates of copepods were species-specific. C. sinicus, which was a macro-copepod, had a higher survival rate （62.01%–71.96%） than the other three species （5.00%–26.67%） during the eight day exposure. The egg production rates of C. sinicus, A. spinicauda and C. tenuiremis were significantly inhibited by the increased p CO2 and the exposure time duration. There were significantly negative impacts on the egg hatching success of A. spinicauda and C. tenuiremis in the p CO2 2 000 × 10 -6 and 10 000 × 10 -6 groups, and, in addition, the exposure time had noticeably impacts on these rates too. This study indicates that the reproductive performances of copepods were sensitive to elevated p CO2 , and that the response of different copepod species to acidified seawater was different. Furthermore, the synergistic effects of seawater acidification and climate change or other pollutant stresses on organisms should be given more attention.
基金This research was supported by the National Natural Science Foundation of China under contract Nos 40276041 and 49976034.
文摘The larvae of ascidian Styela canopus Savigny were treated with epinephrine, norepinephrine, L-DOPA, GABA and thyroxin to test the ability of these compounds to induce or inhibit larval settlement and metamorphosis. The results showed that epinephrine, norepinephrine and L-DOPA at the concentration of 1 μmol/dm^3 induced larval settlement and metamorphosis in S. canopus, with short exposure （ 1 h） to 1 μmol/dm^3 of L-DOPA inducing rapid settlement. In contrast, GABA at the concentrations of 0.1 ～1130.0 μmol/dm^3 significantly inhibited the settlement and metamorphosis of S. canopus larvae. In addition, thyroxin at 1 -50 μg/dm^3 had no effect on larval settlement and metamorphosis in S. canopus. These results suggest the importance of neurotransmitters in the settlement and metamorphosis of S. canopus larvae.
