Response of Lymphocytes to Radiation in Untreated Breast Cancer Patients as Detected with Three Different Genetic Assays

Objective To detect the response of lymphocytes to radiation in untreated breast cancer patients with three different genetic assays. Methods Blood samples were collected from 25 untreated patients and 25 controls. Each blood sample was divided into two parts： one was irradiated by 3-Gy X-ray （irradiated sample）, the other was not irradiated （non-irradiated sample）. The radiosensitivity of lymphocytes was assessed by comet assay, cytokinesis-block micronucleus （CBMN） assay and 6-TG-resistant cells scored （TG） assay. Results The baseline values of micronucleated cell frequency （MCF） and micronucleus frequency （MNF） in the patients were significantly higher than those in the controls （P〈0.01）, and 3-Gy X-ray induced genetic damage to lymphocytes in the patients increased significantly as compared with that in the controls as detected with the three genetic assays （P〈0.01）. The proportion of radiosensitive cases in the patient group was 48% for the mean tail length （MTL）, 40% for the mean tall moment （MTM）, 40% for MCF, 44% for MNF, and 48% for mutation frequencies of the hprt gene （Mfs-hprt）, respectively, whereas the proportion of radiosensitive cases in the control group was only 8% for all the parameters. Conclusion The difference in the lymphocyte radiosensitivity between the breast cancer patients and the controls is significant. Moreover, there are wide individual variations in lymphocyte radiosensitivity of patients with breast cancer. In some cases, the radiosensitivity of the same patient may be different as detected with the different assays. It is suggested that multiple assays should be used to assess the radiosensitivity of patients with breast cancer before therapy.

Comparative efficacy of oral glucose solution versus non-nutritive sucking for pain relief in neonates during nociceptive procedures： a systematic review

背景和目的：葡萄糖溶液与非营养性吸吮对新生儿疼痛缓解的比较效果仍存在争议。因此，我们进行了系统评价以客观地研究在新生儿伤害感受过程中新生儿口服葡萄糖溶液与非营养性吸吮相关的镇痛作用。方法：在PubMed，Web of Science，EMBASE和Cochrane Library中搜索所有潜在记录，以获取2017年1月前比较葡萄糖溶液与非营养性吸吮在新生儿疼痛评分方面的随机对照试验。两名独立研究人员筛选出已鉴定的文章，提取数据，并评估纳入研究的方法学质量。使用Review Manager（RevMan）版本5．3．0完成所有统计分析。结果：本研究对248例新生儿进行 项统计分析。葡萄糖溶液与非营养性吸吮相比在减少疼痛评分方面没有检测到统计学差异（4项试验；MD=0．75；95％CI，-0．77-2．27；P=0．33）。结论：葡萄糖溶液与非营养性吸吮均可降低接受乙型肝炎疫苗接种和静脉穿刺的新生儿的疼痛评分，但非营养性吮吸在一定程度上比葡萄糖溶液更方便。考虑到证据的有限性，有必要进行更多随机对照试验，以及高质量、大规模和适当的测量时间，以进一步确定这两种方案的效果比较。