Be able to form large particles with protein via electrostatic force to enhance the intensity of resonance light scattering（RLS）,（NH4）2SO4 was used as a RLS probe to determine bovine serum albumin（BSA）,human ser...Be able to form large particles with protein via electrostatic force to enhance the intensity of resonance light scattering（RLS）,（NH4）2SO4 was used as a RLS probe to determine bovine serum albumin（BSA）,human serum albumin（HSA） and ovum albumin（OVA）.The experimental conditions were investigated,including the acidity and saturation degree of （NH4）2SO4.Under the optimum conditions,the enhanced RLS intensity is proportional to the concentration of BSA,HSA and OVA in the ranges of 5×10-8―1×10-6,2.5×10-8―8×10-7 and 5×10-8―1.5×10-6 mol/L,respectively.The detection limits for BSA,HAS and OVA are 6.6×10-9,3.8×10-9 and 7.4×10-9 mol/L,respectively.The effects of foreign substances were also examined.The practical and synthetic samples were analyzed with satisfactory results.展开更多
The authors performed ionic liquid-based microwave-assisted liquid-liquid microextraction（IL-based MALLME） coupled with high performance liquid chromatographic separation for the determination of 6 sulfonamides （SA...The authors performed ionic liquid-based microwave-assisted liquid-liquid microextraction（IL-based MALLME） coupled with high performance liquid chromatographic separation for the determination of 6 sulfonamides （SAs） from animal oils. The target analytes were extracted from animal oil samples with sodium hydroxide solution containing 1-butyl-3-methylimidazolium tetrafluoroborateand as the extraction solvent under microwave irradiation. The experimental parameters of the IL-based MALLME, including types of ILs, volume of IL, amount of ion-pairing agent（NHaPF6）, pH value of sample solution, and extraction temperature and time were evaluated. The limits of detection and quantification obtained were in a range of 0.4--0.5μg/kg and a range of 1.2--1.8μg/kg, respectively. The accuracy of the method was evaluated by analyzing five spiked animal oil samples at two fortified levels（5 and 50μg/kg）, and the recoveries of SAs varied from 81.4% to 114.5% with relative standard deviations ranging from 0.8% to 9.0%.展开更多
文摘Be able to form large particles with protein via electrostatic force to enhance the intensity of resonance light scattering（RLS）,（NH4）2SO4 was used as a RLS probe to determine bovine serum albumin（BSA）,human serum albumin（HSA） and ovum albumin（OVA）.The experimental conditions were investigated,including the acidity and saturation degree of （NH4）2SO4.Under the optimum conditions,the enhanced RLS intensity is proportional to the concentration of BSA,HSA and OVA in the ranges of 5×10-8―1×10-6,2.5×10-8―8×10-7 and 5×10-8―1.5×10-6 mol/L,respectively.The detection limits for BSA,HAS and OVA are 6.6×10-9,3.8×10-9 and 7.4×10-9 mol/L,respectively.The effects of foreign substances were also examined.The practical and synthetic samples were analyzed with satisfactory results.
文摘The authors performed ionic liquid-based microwave-assisted liquid-liquid microextraction（IL-based MALLME） coupled with high performance liquid chromatographic separation for the determination of 6 sulfonamides （SAs） from animal oils. The target analytes were extracted from animal oil samples with sodium hydroxide solution containing 1-butyl-3-methylimidazolium tetrafluoroborateand as the extraction solvent under microwave irradiation. The experimental parameters of the IL-based MALLME, including types of ILs, volume of IL, amount of ion-pairing agent（NHaPF6）, pH value of sample solution, and extraction temperature and time were evaluated. The limits of detection and quantification obtained were in a range of 0.4--0.5μg/kg and a range of 1.2--1.8μg/kg, respectively. The accuracy of the method was evaluated by analyzing five spiked animal oil samples at two fortified levels（5 and 50μg/kg）, and the recoveries of SAs varied from 81.4% to 114.5% with relative standard deviations ranging from 0.8% to 9.0%.
