By using OsRacD cDNA as probe to screen the genomic library of photoperiod sensitive genic male sterile rice line Nongken 58S, a positive clone containing 2 kb promoter and 396 bp coding region of OsRacD was obtained....By using OsRacD cDNA as probe to screen the genomic library of photoperiod sensitive genic male sterile rice line Nongken 58S, a positive clone containing 2 kb promoter and 396 bp coding region of OsRacD was obtained. Compared with the promoter of OsRacD cloned by reverse PCR from normal rice variety Nongken 58 （Nongken 58N）, the homology was 99.8%, and the different nucleotides were outside the predicted response elements in promoter, suggesting that the fertility between rice varieties Nongken 58S and Nongken 58N under the long-day conditions was not attributed to the difference in the structure of OsRacD upstream regulation sequences, but to the developmental regulation of gene differential expression.展开更多
The expression patterns of OsPILll, one of six putative phytochrome-interacting factors, were analyzed in different organs of transgenic tobacco （Nicotiana tabacum）. The expression of OsPIL 11 was organ-specific and...The expression patterns of OsPILll, one of six putative phytochrome-interacting factors, were analyzed in different organs of transgenic tobacco （Nicotiana tabacum）. The expression of OsPIL 11 was organ-specific and was regulated by leaf development, abscisic acid （ABA）, jasmonic acid （JA） and salicylic acid （SA）. To further explore the role of OsPIL 11 in plant light signal transduction, a plant expression vector of OsPILll was constructed and introduced into tobacco. When grown under continuous red light, OsPILll-overexpressed transgenic tobacco exhibited shorter hypocotyls and larger cotyledons and leaves compared to wild-type seedlings. When grown under continuous far-red light, however, transgenic and wild-type seedlings showed similar phenotypes. These results indicate that OsPILll is involved in red light induced de-etiolation, but not in far-red light induced de-etiolation in transgenic tobacco, which lays the foundation for dissecting the function of OsPIL11 in phytochrome-mediated light signal transduction in rice.展开更多
We re-study nucleon pole contribution in J/ψ → NN^-π decays by including the imaginary part for the propagator of the off-shell nucleon with energy above π N threshold. It is found that when including the imaginar...We re-study nucleon pole contribution in J/ψ → NN^-π decays by including the imaginary part for the propagator of the off-shell nucleon with energy above π N threshold. It is found that when including the imaginary part in the propagator, the branching ratio of the decay width will descend about 11% compared with the result without including the imaginary part, no matter whether including the off-shell form factors or not. It also leads to a phase of up to 25° for the off-shell nucleon propagator at invariant mass around 1400 MeV. This effect needs to be considered for detailed partial wave analysis of N^＊ resonances around this mass region.展开更多
文摘By using OsRacD cDNA as probe to screen the genomic library of photoperiod sensitive genic male sterile rice line Nongken 58S, a positive clone containing 2 kb promoter and 396 bp coding region of OsRacD was obtained. Compared with the promoter of OsRacD cloned by reverse PCR from normal rice variety Nongken 58 （Nongken 58N）, the homology was 99.8%, and the different nucleotides were outside the predicted response elements in promoter, suggesting that the fertility between rice varieties Nongken 58S and Nongken 58N under the long-day conditions was not attributed to the difference in the structure of OsRacD upstream regulation sequences, but to the developmental regulation of gene differential expression.
基金supported by grants from the Chinese National Natural Science Foundation (Grant No. 30971744)the Chinese Ministry of Agriculture(Grant No. 2009ZX08001-029B)the Shandong Natural Science Funds for Distinguished Young Scholar,China (Grant No. JQ200911)
文摘The expression patterns of OsPILll, one of six putative phytochrome-interacting factors, were analyzed in different organs of transgenic tobacco （Nicotiana tabacum）. The expression of OsPIL 11 was organ-specific and was regulated by leaf development, abscisic acid （ABA）, jasmonic acid （JA） and salicylic acid （SA）. To further explore the role of OsPIL 11 in plant light signal transduction, a plant expression vector of OsPILll was constructed and introduced into tobacco. When grown under continuous red light, OsPILll-overexpressed transgenic tobacco exhibited shorter hypocotyls and larger cotyledons and leaves compared to wild-type seedlings. When grown under continuous far-red light, however, transgenic and wild-type seedlings showed similar phenotypes. These results indicate that OsPILll is involved in red light induced de-etiolation, but not in far-red light induced de-etiolation in transgenic tobacco, which lays the foundation for dissecting the function of OsPIL11 in phytochrome-mediated light signal transduction in rice.
基金The project partly supported by National Natural Science Foundation of China under Grant Nos. 10475089, 10435080, 10447002, 10147202, and 10225525, the Physical Science Fund of Guangxi Province under Grant No. 9714005, and the Knowledge Innovation Key Project of the Chinese Academy of Sciences under Grant No. KJCX2-SW-N02.
文摘We re-study nucleon pole contribution in J/ψ → NN^-π decays by including the imaginary part for the propagator of the off-shell nucleon with energy above π N threshold. It is found that when including the imaginary part in the propagator, the branching ratio of the decay width will descend about 11% compared with the result without including the imaginary part, no matter whether including the off-shell form factors or not. It also leads to a phase of up to 25° for the off-shell nucleon propagator at invariant mass around 1400 MeV. This effect needs to be considered for detailed partial wave analysis of N^＊ resonances around this mass region.
