Stutzerimonas have been extensively studied due to their remarkable metabolic and physiological diversity.However,research on its phages is currently limited.In this study,we isolated a novel double-stranded DNA(dsDNA...Stutzerimonas have been extensively studied due to their remarkable metabolic and physiological diversity.However,research on its phages is currently limited.In this study,we isolated a novel double-stranded DNA(dsDNA)phage,vB_SstM-PG1,from the marine environment that infects Stutzerimonas stutzeri G1.Its dsDNA genome is 37204 bp long with a G/C content of 64.14%and encodes 54 open reading frames.The phage possesses a tail packaging structure that is different from known Stutzerimonas stutzeri phages and exhibits structural protein characteristics similar to those of temperate phages.In addition,two genes of toxin-antitoxin system,including YdaS_antitoxin and HEPN_SAV_6107,were found in the vB_SstM-PG1 genome and play important roles in regulating host growth and metabolism.With phylogenetic tree and comparative genomic analysis,it has been determined that vB_SstM-PG1 is not closely related to any phages previously identified in the GenBank database.Instead,it has a connection with enigmatic,uncultured viruses.Specifically,the vB_SstM-PG1 virus exhibits an average nucleotide identity of over 70%with six uncultivated viruses identified in the IMG/VR v4 database.This significant finding has resulted in the identification of a novel viral genus known as Metabovirus.展开更多
BMR (basal metabolic rate), body mass and organ masses of tree sparrows (Passer montanus) were measured to analyze the correlation between organ masses and BMR in tree sparrows, and to evaluate the underlying phys...BMR (basal metabolic rate), body mass and organ masses of tree sparrows (Passer montanus) were measured to analyze the correlation between organ masses and BMR in tree sparrows, and to evaluate the underlying physiological causes of difference in BMR. Adult tree sparrows were live-trapped by mist net in Qiqihar City, Heilongjiang Province (47°29′N, 124°02′E). The closed circuit respirometer was used to measure the metabolic rate (MR), and controlled the ambient temperature by using a water bath (±0.5℃). Body masses were measured to the nearest 0.01 g before and after BMR measurements with a Sartorius balance (model BT25S). The mean value was recorded as body mass. Wet and dry masses of several organs were measured, too. BMR was (4.276± 0.385) mL O2/(g·h) and mean body mass was (18.522±0.110) g. Since not all the variables were normal distributed, a log10- transformation of those variables was employed to linearize them, prior to analyses. Simple regression analyses indicated that most organ masses showed a significant high correlation with body mass. Both the small intestine and rectum masses were notable exception to that trend. The body-mass-adjusted residual analysis showed that only the kidney wet mass, brain mass, stomach mass, small mass and rectum wet mass correlated with BMR. In addition, correlations between several organ masses and BMR were observed. Because of the inter-correlations of organ masses, a principal component analysis (PCA) was performed to redefine the morphological variability. The first four components whose eigenvalues were greater than 1 could explain 75.2% variance of BMR. The first component, whose proportion reached 30.19%, was affected mainly by stomach mass, small intestine mass and rectum mass. Therefore, the results supported the hypothesis that BMR was controlled by some "expensive metabolic" organs展开更多
In order to understand the large-scale spatial distribution characteristics of picoplankton,nanophytoplankton and virio-plankton and their relationship with environmental variables in coastal and offshore waters,flow ...In order to understand the large-scale spatial distribution characteristics of picoplankton,nanophytoplankton and virio-plankton and their relationship with environmental variables in coastal and offshore waters,flow cytometry(FCM) was used to ana-lyze microbial abundance of samples collected in summer from four depths at 36 stations in the North Yellow Sea(NYS).The data revealed spatial heterogeneity in microbial populations in the offshore and near-shore waters of the NYS during the summer.For the surface layer,picoeukaryotes were abundant in the near-shore waters,Synechococcus was abundant in the offshore areas,and bacte-rial and viral abundances were high in the near-shore waters around the Liaodong peninsula.In the near-shore waters,no significant vertical variation of picophytoplankton(0.2-2μm) abundance was found.However,the nanophytoplankton abundance was higher in the upper layers(from the surface to 10 m depth) than in the bottom layer.For the offshore waters,both pico-and nanophytoplankton(2-20μm) abundance decreased sharply with depth in the North Yellow Sea Cold Water Mass(NYSCWM).But,for the vertical dis-tribution of virus and bacteria abundance,no significant variation was observed in both near-shore and offshore waters.Autotrophic microbes were more sensitive to environmental change than heterotrophic microbes and viruses.Viruses showed a positive correla-tion with bacterial abundance,suggesting that the bacteriophage might be prominent for virioplankton(about 0.45μm) in summer in the NYS and that viral abundance might play an important role in microbial loop functions.展开更多
基金supported by the National Natural Science Foundation of China (Nos.42188102,42120104006,41976117,42176111 and 42306111)the Fundamental Research Funds for the Central Universities (No.201812002 and Andrew McMinn)。
文摘Stutzerimonas have been extensively studied due to their remarkable metabolic and physiological diversity.However,research on its phages is currently limited.In this study,we isolated a novel double-stranded DNA(dsDNA)phage,vB_SstM-PG1,from the marine environment that infects Stutzerimonas stutzeri G1.Its dsDNA genome is 37204 bp long with a G/C content of 64.14%and encodes 54 open reading frames.The phage possesses a tail packaging structure that is different from known Stutzerimonas stutzeri phages and exhibits structural protein characteristics similar to those of temperate phages.In addition,two genes of toxin-antitoxin system,including YdaS_antitoxin and HEPN_SAV_6107,were found in the vB_SstM-PG1 genome and play important roles in regulating host growth and metabolism.With phylogenetic tree and comparative genomic analysis,it has been determined that vB_SstM-PG1 is not closely related to any phages previously identified in the GenBank database.Instead,it has a connection with enigmatic,uncultured viruses.Specifically,the vB_SstM-PG1 virus exhibits an average nucleotide identity of over 70%with six uncultivated viruses identified in the IMG/VR v4 database.This significant finding has resulted in the identification of a novel viral genus known as Metabovirus.
基金Supported by Natural Foundation for Youth of Daqing Normal College (YZQ004)
文摘BMR (basal metabolic rate), body mass and organ masses of tree sparrows (Passer montanus) were measured to analyze the correlation between organ masses and BMR in tree sparrows, and to evaluate the underlying physiological causes of difference in BMR. Adult tree sparrows were live-trapped by mist net in Qiqihar City, Heilongjiang Province (47°29′N, 124°02′E). The closed circuit respirometer was used to measure the metabolic rate (MR), and controlled the ambient temperature by using a water bath (±0.5℃). Body masses were measured to the nearest 0.01 g before and after BMR measurements with a Sartorius balance (model BT25S). The mean value was recorded as body mass. Wet and dry masses of several organs were measured, too. BMR was (4.276± 0.385) mL O2/(g·h) and mean body mass was (18.522±0.110) g. Since not all the variables were normal distributed, a log10- transformation of those variables was employed to linearize them, prior to analyses. Simple regression analyses indicated that most organ masses showed a significant high correlation with body mass. Both the small intestine and rectum masses were notable exception to that trend. The body-mass-adjusted residual analysis showed that only the kidney wet mass, brain mass, stomach mass, small mass and rectum wet mass correlated with BMR. In addition, correlations between several organ masses and BMR were observed. Because of the inter-correlations of organ masses, a principal component analysis (PCA) was performed to redefine the morphological variability. The first four components whose eigenvalues were greater than 1 could explain 75.2% variance of BMR. The first component, whose proportion reached 30.19%, was affected mainly by stomach mass, small intestine mass and rectum mass. Therefore, the results supported the hypothesis that BMR was controlled by some "expensive metabolic" organs
基金supported by the General Oceano-graphic Survey Project(908 Project)the Special Fund forPublic Welfare Industry(Oceanography)(Grant No.20080511)the CAS Hundred Talents Project‘The response mechanism of the typical gulf ecosystem to the environmental changes’
文摘In order to understand the large-scale spatial distribution characteristics of picoplankton,nanophytoplankton and virio-plankton and their relationship with environmental variables in coastal and offshore waters,flow cytometry(FCM) was used to ana-lyze microbial abundance of samples collected in summer from four depths at 36 stations in the North Yellow Sea(NYS).The data revealed spatial heterogeneity in microbial populations in the offshore and near-shore waters of the NYS during the summer.For the surface layer,picoeukaryotes were abundant in the near-shore waters,Synechococcus was abundant in the offshore areas,and bacte-rial and viral abundances were high in the near-shore waters around the Liaodong peninsula.In the near-shore waters,no significant vertical variation of picophytoplankton(0.2-2μm) abundance was found.However,the nanophytoplankton abundance was higher in the upper layers(from the surface to 10 m depth) than in the bottom layer.For the offshore waters,both pico-and nanophytoplankton(2-20μm) abundance decreased sharply with depth in the North Yellow Sea Cold Water Mass(NYSCWM).But,for the vertical dis-tribution of virus and bacteria abundance,no significant variation was observed in both near-shore and offshore waters.Autotrophic microbes were more sensitive to environmental change than heterotrophic microbes and viruses.Viruses showed a positive correla-tion with bacterial abundance,suggesting that the bacteriophage might be prominent for virioplankton(about 0.45μm) in summer in the NYS and that viral abundance might play an important role in microbial loop functions.