The semidwarf gene sd-g which has been used in indica rice breeding in southern China is a new one, non-allelic to sd-1. To map sd-g, an F2 population derived from the cross between Xinguiaishuangai and 02428 was con-...The semidwarf gene sd-g which has been used in indica rice breeding in southern China is a new one, non-allelic to sd-1. To map sd-g, an F2 population derived from the cross between Xinguiaishuangai and 02428 was con-structed. The sd-g was roughly mapped between two mi-crosatellite markers RM440 and RM163, with genetic dis-tances of 0.5 and 2.5 cM, respectively. Then nine new poly-morphic microsatellite markers were developed in this region. The sd-g was further mapped between two microsatellite markers SSR5-1 and SSR5-51, with genetic distances of 0.1 and 0.3 cM, respectively, while cosegregated with SSR418. A BAC contig was found to span the sd-g locus, the region be-ing delimited to 85 kb. This result was very useful for cloning of the sd-g gene.展开更多
文摘The semidwarf gene sd-g which has been used in indica rice breeding in southern China is a new one, non-allelic to sd-1. To map sd-g, an F2 population derived from the cross between Xinguiaishuangai and 02428 was con-structed. The sd-g was roughly mapped between two mi-crosatellite markers RM440 and RM163, with genetic dis-tances of 0.5 and 2.5 cM, respectively. Then nine new poly-morphic microsatellite markers were developed in this region. The sd-g was further mapped between two microsatellite markers SSR5-1 and SSR5-51, with genetic distances of 0.1 and 0.3 cM, respectively, while cosegregated with SSR418. A BAC contig was found to span the sd-g locus, the region be-ing delimited to 85 kb. This result was very useful for cloning of the sd-g gene.
