目的:系统评价百合固金汤治疗肺结核的临床疗效及对肺部病灶的影响。方法:检索中国期刊全文数据库(CNKI)、万方(Wanfang),维普数据库(VIP))和中国生物医学文摘数据库(CBM),收集百合固金汤联合西药治疗肺结核的随机或半随机对照试验(RCTs...目的:系统评价百合固金汤治疗肺结核的临床疗效及对肺部病灶的影响。方法:检索中国期刊全文数据库(CNKI)、万方(Wanfang),维普数据库(VIP))和中国生物医学文摘数据库(CBM),收集百合固金汤联合西药治疗肺结核的随机或半随机对照试验(RCTs or CCTs),采用Cochrane风险偏倚评估工具对所纳入文献进行偏倚风险评估,并对临床总有效率、病灶吸收率、痰菌转阴率、空洞闭合有效率进行Meta分析。结果:纳入28篇文献,共2,844例患者,Meta分析结果显示,治疗组临床总有效率[RR=1.28,95%CI(1.23,1.33)],病灶吸收率[RR=1.28,95%CI(1.20,1.36)],痰菌转阴率[RR=1.21,95%CI(1.12,1.30)],空洞闭合率[RR=1.23,95%CI(1.10,1.37)]均优于对照组,且不良反应少。结论:百合固金汤联合西药治疗肺结核可提高临床疗效和改善肺部病灶情况,由于纳入文献研究方法学和报告质量较低,样本量少,仍需要严格、多中心、大样本的随机双盲对照试验加以验证。展开更多
目的:对使用复方丹参滴丸联合西药常规治疗心力衰竭的临床研究进行Meta分析,评价其临床疗效。方法:通过检索Pub Med、Coc hra n e临床对照试验中心注册库(CE NTRAL)、荷兰医学文摘(E m ba se)、中国知网(CNKI)、万方(Wa n Fa n g)、维普...目的:对使用复方丹参滴丸联合西药常规治疗心力衰竭的临床研究进行Meta分析,评价其临床疗效。方法:通过检索Pub Med、Coc hra n e临床对照试验中心注册库(CE NTRAL)、荷兰医学文摘(E m ba se)、中国知网(CNKI)、万方(Wa n Fa n g)、维普(VIP)和中国生物医学文摘(CBM)等数据库,选择符合纳入标准的研究,进行Meta分析。结果:共纳入21个随机对照试验(RCTs),16 9 1例患者。通过牛津评分系统Ja da d标准量表进行文献质量评价,显示均为低质量文章。Meta分析结果显示:治疗心力衰竭的实验观察指标显示西药常规联合复方丹参滴丸,能提高治疗心力衰竭的有效率,临床综合疗效[相对危险度(RR)=1.21,9 5%可信区间(CI)(1.16,1.27)],左室射血分数[均数差(MD)=4.6 7,9 5%CI(3.3 1,6.02)],6 m i n步行距离[MD=4 4.89,9 5%CI(3 2.59,57.18)],B型脑钠肽[MD=-29 7.84,9 5%CI(-24 1.3 5,-154.3 3)]等指标上均表明比单用西药治疗好,差异均有统计学意义(P<0.05)。在安全性方面[RR=1.25,9 5%CI(0.57,2.75)],差异无统计学意义(P>0.05)。结论:复方丹参滴丸联合西药常规治疗心力衰竭的临床疗效优于单用西药,安全性相当。展开更多
Objective To observe the effects of Centipede Scolopendra extraction(CSE)on human liver cancer HepG2 cells and the nude mouse tumor model of liver orthotopic transplantation,and to explore the anti-liver cancer mechan...Objective To observe the effects of Centipede Scolopendra extraction(CSE)on human liver cancer HepG2 cells and the nude mouse tumor model of liver orthotopic transplantation,and to explore the anti-liver cancer mechanism of the extract.Methods HepG2 cells were respectively treated with CSE250(250μg/mL),CSE500(500μg/mL)and 5-FU,and control group was established.An enzymatic hydrolysis and acetone precipitation method was used to separate and purify CSE,which was then used to treat HepG2 cells.The CCK8 assay was used to detect the inhibition of cell proliferation and the half maximal inhibitory concentration(IC50)was calculated.Flow cytometry was used to analyze the cell cycle,and western blot was used to detect the expression of signal transduction and activator of transcription 3(STAT3)pathway-related proteins in HepG2 cells treated with CSE.A nude mouse model with an orthotopic liver tumor was prepared.The mice were randomly divided into four groups,each containing 12 animals:the model group,the 5-FU group,the CSE10 group[10 mg/(kg·d)]and the CSE50 group[50 mg/(kg·d)].The volume and mass changes in the nude mice with orthotopic transplanted tumors were observed.Western blot method was used to test the protein expression levels of p-STAT3 and p38 mitogen-activated protein kinase(p38MAPK).Tissues from the liver of mice in the model group and the CSE50 group were analyzed by using a protein tyrosine kinase(PTK)chip,and the Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)function enrichment analysis of the differentially expressed proteins was performed.Results This study showed that CSE significantly inhibited the proliferation of HepG2 cells(P<0.05).After 48 h of CSE treatment,the cell cycle of HepG2 cells manifested as S phase and G2/M phase;p-STAT3 protein levels in the CSE groups were significantly lower than that in the control group(P<0.05).Analysis of the tumor inhibition in the mice showed that the tumor masses and volume in CSE groups were lower(P<0.05).The protein levels of p-STAT3 and p38MAPK in CSE50 group and 5-FU group decreased significantly(P<0.05).PTK antibody chip screening results showed that CSE groups had a bidirectional regulation trend,and there were 23 up-regulated PTKs and six down-regulated PTKs.The GO and KEGG analyses showed that CSE exerted its anticancer effects through regulation of biological processes,including mitogen-activated protein kinase(MAPK)cascade,chemotaxis,cell invasion,cell adhesion,angiogenesis and other biological processes,and through signaling pathways,including the MAPK,phosphatidylinositol-3-kinase/serine threonine protein kinase(PI3K/AKT),and RAS signaling pathways.Conclusions CSE can effectively inhibite the proliferation of HepG2 cells and effectively inhibite the growth of liver cancer orthotopic transplantation tumor.Its mechanism may be closely related to the regulation of STAT3,MAPK and PI3K/AKT signaling pathways.展开更多
文摘目的:系统评价百合固金汤治疗肺结核的临床疗效及对肺部病灶的影响。方法:检索中国期刊全文数据库(CNKI)、万方(Wanfang),维普数据库(VIP))和中国生物医学文摘数据库(CBM),收集百合固金汤联合西药治疗肺结核的随机或半随机对照试验(RCTs or CCTs),采用Cochrane风险偏倚评估工具对所纳入文献进行偏倚风险评估,并对临床总有效率、病灶吸收率、痰菌转阴率、空洞闭合有效率进行Meta分析。结果:纳入28篇文献,共2,844例患者,Meta分析结果显示,治疗组临床总有效率[RR=1.28,95%CI(1.23,1.33)],病灶吸收率[RR=1.28,95%CI(1.20,1.36)],痰菌转阴率[RR=1.21,95%CI(1.12,1.30)],空洞闭合率[RR=1.23,95%CI(1.10,1.37)]均优于对照组,且不良反应少。结论:百合固金汤联合西药治疗肺结核可提高临床疗效和改善肺部病灶情况,由于纳入文献研究方法学和报告质量较低,样本量少,仍需要严格、多中心、大样本的随机双盲对照试验加以验证。
文摘目的:对使用复方丹参滴丸联合西药常规治疗心力衰竭的临床研究进行Meta分析,评价其临床疗效。方法:通过检索Pub Med、Coc hra n e临床对照试验中心注册库(CE NTRAL)、荷兰医学文摘(E m ba se)、中国知网(CNKI)、万方(Wa n Fa n g)、维普(VIP)和中国生物医学文摘(CBM)等数据库,选择符合纳入标准的研究,进行Meta分析。结果:共纳入21个随机对照试验(RCTs),16 9 1例患者。通过牛津评分系统Ja da d标准量表进行文献质量评价,显示均为低质量文章。Meta分析结果显示:治疗心力衰竭的实验观察指标显示西药常规联合复方丹参滴丸,能提高治疗心力衰竭的有效率,临床综合疗效[相对危险度(RR)=1.21,9 5%可信区间(CI)(1.16,1.27)],左室射血分数[均数差(MD)=4.6 7,9 5%CI(3.3 1,6.02)],6 m i n步行距离[MD=4 4.89,9 5%CI(3 2.59,57.18)],B型脑钠肽[MD=-29 7.84,9 5%CI(-24 1.3 5,-154.3 3)]等指标上均表明比单用西药治疗好,差异均有统计学意义(P<0.05)。在安全性方面[RR=1.25,9 5%CI(0.57,2.75)],差异无统计学意义(P>0.05)。结论:复方丹参滴丸联合西药常规治疗心力衰竭的临床疗效优于单用西药,安全性相当。
基金funding support from the National Natural Science Foundation of China(No.81473617)the Science and Technology Department of Hunan Province(No.2017SK50310)the Hunan Education Department’s Science&Research Project(No.16K066)。
文摘Objective To observe the effects of Centipede Scolopendra extraction(CSE)on human liver cancer HepG2 cells and the nude mouse tumor model of liver orthotopic transplantation,and to explore the anti-liver cancer mechanism of the extract.Methods HepG2 cells were respectively treated with CSE250(250μg/mL),CSE500(500μg/mL)and 5-FU,and control group was established.An enzymatic hydrolysis and acetone precipitation method was used to separate and purify CSE,which was then used to treat HepG2 cells.The CCK8 assay was used to detect the inhibition of cell proliferation and the half maximal inhibitory concentration(IC50)was calculated.Flow cytometry was used to analyze the cell cycle,and western blot was used to detect the expression of signal transduction and activator of transcription 3(STAT3)pathway-related proteins in HepG2 cells treated with CSE.A nude mouse model with an orthotopic liver tumor was prepared.The mice were randomly divided into four groups,each containing 12 animals:the model group,the 5-FU group,the CSE10 group[10 mg/(kg·d)]and the CSE50 group[50 mg/(kg·d)].The volume and mass changes in the nude mice with orthotopic transplanted tumors were observed.Western blot method was used to test the protein expression levels of p-STAT3 and p38 mitogen-activated protein kinase(p38MAPK).Tissues from the liver of mice in the model group and the CSE50 group were analyzed by using a protein tyrosine kinase(PTK)chip,and the Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)function enrichment analysis of the differentially expressed proteins was performed.Results This study showed that CSE significantly inhibited the proliferation of HepG2 cells(P<0.05).After 48 h of CSE treatment,the cell cycle of HepG2 cells manifested as S phase and G2/M phase;p-STAT3 protein levels in the CSE groups were significantly lower than that in the control group(P<0.05).Analysis of the tumor inhibition in the mice showed that the tumor masses and volume in CSE groups were lower(P<0.05).The protein levels of p-STAT3 and p38MAPK in CSE50 group and 5-FU group decreased significantly(P<0.05).PTK antibody chip screening results showed that CSE groups had a bidirectional regulation trend,and there were 23 up-regulated PTKs and six down-regulated PTKs.The GO and KEGG analyses showed that CSE exerted its anticancer effects through regulation of biological processes,including mitogen-activated protein kinase(MAPK)cascade,chemotaxis,cell invasion,cell adhesion,angiogenesis and other biological processes,and through signaling pathways,including the MAPK,phosphatidylinositol-3-kinase/serine threonine protein kinase(PI3K/AKT),and RAS signaling pathways.Conclusions CSE can effectively inhibite the proliferation of HepG2 cells and effectively inhibite the growth of liver cancer orthotopic transplantation tumor.Its mechanism may be closely related to the regulation of STAT3,MAPK and PI3K/AKT signaling pathways.
