经导管主动脉瓣置换术患者的护理效果观察

目的:回顾总结16例经导管主动脉瓣置换术(transcatheter aortic valve replacement,TAVR)患者围手术期的护理措施。方法:对2019年12月—2021年1月在本院接受TAVR治疗的16例患者进行回顾性分析,对围手术期护理措施和方法进行总结分析。结果:16例患者中有15例手术成功。结论:通过术前的全面评估及详细宣教,术中的密切观察及配合,术后的严密监护,预防和及时处理并发症,术前、术后心脏康复护理以及患者出院后的延伸护理服务,可以增加TAVR患者手术的成功率,促进TAVR患者的早期康复。

艾司洛尔在急性非ST段抬高型心肌梗死中早期应用的疗效及安全性探究

目的:探讨艾司洛尔在急性非ST段抬高型心肌梗死早期应用的疗效及安全性。方法:选取2016年6月-2018年8月我院收治的急性非ST段抬高型心肌梗死患者143例,随机分为观察组(静脉泵入艾司洛尔+口服β受体阻滞剂)64例和对照组(常规口服β受体阻滞剂)79例,比较两组治疗前后胸痛缓解情况、心电图ST段恢复程度及其并发症的情况。结果:观察组总有效率为89.1%,对照组为73.4%,差异有统计学意义(P<0.05)。治疗前,两组的ST段压低水平无统计学差异(P>0.05);治疗后,两组ST段均有不同程度的恢复,观察组恢复程度更大(P<0.05),对照组不良反应事件较观察组多,但差异无统计学意义(P>0.05)。结论:艾司洛尔在急性非ST段抬高型心肌梗死中早期应用疗效显著,安全可靠。

2种侧围加强内框总成检具结构对比分析

概述了侧围加强内框总成与周边零件的匹配关系,从检具操作的人机工程出发,描述了侧围加强内框总成检具的2种定位方式和结构设计,对比了2种定位方式对于检测结果的影响,总结了其优点和弊端,结果表明侧围加强内框总成检具采用车内方向朝下的定位方式效果更好。

枸骨法尼基焦磷酸合酶IcFPS2基因克隆与原核表达分析

目的 克隆枸骨Ilex cornuta三萜皂苷生物合成途径中关键酶法尼基焦磷酸合酶(farnesyl diphosphate synthase,FPS)基因,对其进行组织特异性表达分析,构建原核表达载体并进行重组蛋白诱导表达,探究其参与调控三萜皂苷生物合成的功能。方法 结合枸骨转录组数据设计特异性引物,采用PCR技术从枸骨叶中扩增得到了IcFPS2基因的c DNA序列,对其进行生物信息学分析;通过实时荧光定量进一步分析其组织表达特异性,构建原核表达载体pET32a-IcFPS2,并转化至大肠杆菌E. coli BL21(DE3)pLysS感受态细胞,经异丙基-β-D-硫代半乳糖苷(IPTG)诱导重组蛋白的表达。结果 IcFPS2基因长1259 bp,开放阅读框为1050 bp,编码350个氨基酸,其蛋白质相对分子质量和等电点分别为40 200、5.54。氨基酸序列比对分析表明枸骨与栀子、杜仲、甘草、绞股蓝、竹节参的FPS氨基酸序列具有较高的同源性。系统进化树分析显示,IcFPS2蛋白与西洋参、人参、竹节参FPS蛋白聚为一支,表明枸骨FPS蛋白可能与双子叶植物五加科FPS蛋白在功能上较为接近。实时荧光定量PCR分析表明,IcFPS2基因在根中的表达量最高,其次是叶,而后是雄花和茎,该基因在雌花中表达水平最低。原核表达分析结果显示,构建的IcFPS2-pET-32a载体能在大肠杆菌BL21(DE3)中成功表达,SDS-PAGE结果显示,诱导的重组表达蛋白相对分子质量在45000左右,与预测的IcFPS2蛋白大小基本一致。结论 通过对IcFPS2基因的全长c DNA克隆与生物信息学分析、组织表达特异性分析和原核表达载体的构建,为后续进一步研究法尼基焦磷酸合酶基因在枸骨三萜皂苷生物合成途径的功能供科学依据。

Left ventricular function in rats measured by apical catheterization

Background Evaluation of left ventricular function plays a vital role in the study of hemodynamics in the circulatory system.This study investigated the feasibility of measuring left ventricular function in rats with catheterization at the apex of the heart.Methods Left carotid catheterization was used to monitor artery pressure,then right carotid-ventricular catheterization and apical catheterization were orderly performed in 20 SD rats to observe the changes of left heart function.Heart rate,carotid arterial pressure,left ventricular peak pressure,left ventricular end-diastolic pressure were recorded by the biological function experiment system,and differential calculations analyzed the systolic and diastolic rate of left ventricular pressure.Results The peak left ventricular pressure was statistically different between when measured by right carotid-ventricle catheterization and by catheterization at the apex of heart(120.7±14.46 mmHg vs.114.04±12.01 mmHg,P<0.05).There was a significant difference in the carotid artery pressure between when the left ventricular function was measured by right carotid-ventricular catheterization and by apical catheterization(115.6±11.24 mmHg vs.108.07±12.33 mmHg,P<0.05),while a different statistical scenario existed for the maximum increase rate of left ventricular pressure(6197.89±1123.28 mmHg/s vs.5985.92±1342.81 mmHg/s,P>0.05);30 min later,the left ventricular pressure and the maximum increase rate of left ventricular pressure were not significantly different between the first and second measurement measured by catheterization at the apex of heart(114.04±12.01 mmHg vs.112.06±11.26 mmHg,5985.92±1342.81 mmHg/s vs.5700.86±598.97 mmHg/s,P>0.05).However,the maximum decline rate of left ventricular pressure was slightly lower compared with the previous measurement(5231.14±1302.57 mmHg/s vs.4862.72±610.49 mmHg/s,P<0.05).Conclusions It is feasible to evaluate cardiac function in rats by catheterization at the apex of heart,and it is superior to right carotid catheterization for its simpler operation,less bleeding risk,less operation time,and the measurement can be repeatedly conducted at least once.[S Chin J Cardiol 2021;22(1):50-56]