Objective To investigate the active components and mechanism of Sanao Decoction(三拗汤,SAD)in treating chronic cough based on network pharmacology and molecular docking.Methods Active components and their targets were...Objective To investigate the active components and mechanism of Sanao Decoction(三拗汤,SAD)in treating chronic cough based on network pharmacology and molecular docking.Methods Active components and their targets were obtained from the Traditional Chinese Medicine Systems and Pharmacology Database and Analysis Platform(TCMSP),Bioinformatics Analysis Tool for Molecular mech ANism of Traditional Chinese Medicine(BATMAN-TCM)database,and the literature.The component-target regulatory network and protein-protein interaction(PPI)network were constructed by Cytoscape 3.7.2,and a bioinformatics analysis was performed to identify the significant pathways and their relevant targets.Molecular docking of the core active components and relevant targets was performed.Results A total of 98 active components of SAD and the corresponding 113 drug targets were identified.The component-target regulatory network and PPI network were successfully established.Results of the bioinformatics analysis indicated that 2281 Gene Ontology(GO)terms were enriched in chronic cough,including 2062 terms were in biological processes,77 in cellular components,and 142 in molecular functions,and top 20 significant pathways in Kyoto Encyclopedia of Genes and Genomes(KEGG)analysis.Molecular docking study demonstrated that quercetin,luteolin,kaempferol,and naringenin were in good agreement with the corresponding targets.Conclusion The active compounds of SAD,such as quercetin,luteolin,kaempferol,and naringenin,may act on AKT1,MAPK1,RELA,EGFR,and Bcl-2 and regulate the PI3 K-Akt signaling pathway,AGE-RAGE signaling pathway,and fluid shear stress and atherosclerosis pathway to exert the effects of anti-inflammatory,anti-airway remodeling,anti-oxidant stress effects,and repair airway damage,thus treating chronic cough.展开更多
以中国知网(CNKI)收录的1974-2018年我国羽毛球领域研究在体育类核心期刊上发表的411篇文献为数据,利用引文分析软件(Cite Space V)对数据进行可视化分析,绘制国内羽毛球领域研究的知识图谱,描述和分析我国羽毛球领域研究的发展特点,研...以中国知网(CNKI)收录的1974-2018年我国羽毛球领域研究在体育类核心期刊上发表的411篇文献为数据,利用引文分析软件(Cite Space V)对数据进行可视化分析,绘制国内羽毛球领域研究的知识图谱,描述和分析我国羽毛球领域研究的发展特点,研究热点和研究现状。研究表明:我国羽毛球领域的主要研究机构集中在以上海体育学院为主体的体育院校。羽毛球的教学、训练和竞赛人才培养一直是我国羽毛球领域相关机构和学者的研究热点,近年来我国的研究不断细化、深入,注重定量的实验研究,但是仍存在研究方法单一和与其他学科交叉过少等问题。展开更多
基金funding support from the National Natural Science Foundation of China(No.82174093)。
文摘Objective To investigate the active components and mechanism of Sanao Decoction(三拗汤,SAD)in treating chronic cough based on network pharmacology and molecular docking.Methods Active components and their targets were obtained from the Traditional Chinese Medicine Systems and Pharmacology Database and Analysis Platform(TCMSP),Bioinformatics Analysis Tool for Molecular mech ANism of Traditional Chinese Medicine(BATMAN-TCM)database,and the literature.The component-target regulatory network and protein-protein interaction(PPI)network were constructed by Cytoscape 3.7.2,and a bioinformatics analysis was performed to identify the significant pathways and their relevant targets.Molecular docking of the core active components and relevant targets was performed.Results A total of 98 active components of SAD and the corresponding 113 drug targets were identified.The component-target regulatory network and PPI network were successfully established.Results of the bioinformatics analysis indicated that 2281 Gene Ontology(GO)terms were enriched in chronic cough,including 2062 terms were in biological processes,77 in cellular components,and 142 in molecular functions,and top 20 significant pathways in Kyoto Encyclopedia of Genes and Genomes(KEGG)analysis.Molecular docking study demonstrated that quercetin,luteolin,kaempferol,and naringenin were in good agreement with the corresponding targets.Conclusion The active compounds of SAD,such as quercetin,luteolin,kaempferol,and naringenin,may act on AKT1,MAPK1,RELA,EGFR,and Bcl-2 and regulate the PI3 K-Akt signaling pathway,AGE-RAGE signaling pathway,and fluid shear stress and atherosclerosis pathway to exert the effects of anti-inflammatory,anti-airway remodeling,anti-oxidant stress effects,and repair airway damage,thus treating chronic cough.
文摘以中国知网(CNKI)收录的1974-2018年我国羽毛球领域研究在体育类核心期刊上发表的411篇文献为数据,利用引文分析软件(Cite Space V)对数据进行可视化分析,绘制国内羽毛球领域研究的知识图谱,描述和分析我国羽毛球领域研究的发展特点,研究热点和研究现状。研究表明:我国羽毛球领域的主要研究机构集中在以上海体育学院为主体的体育院校。羽毛球的教学、训练和竞赛人才培养一直是我国羽毛球领域相关机构和学者的研究热点,近年来我国的研究不断细化、深入,注重定量的实验研究,但是仍存在研究方法单一和与其他学科交叉过少等问题。
