With sodium dodecyl sulfonate(SDS) as the phase transferring catalyst, N,N-Dilauryl chitosans with a high degree of alkyl group substitution were prepared and characterized by means of FTIR, 1H NMR and elemental ana...With sodium dodecyl sulfonate(SDS) as the phase transferring catalyst, N,N-Dilauryl chitosans with a high degree of alkyl group substitution were prepared and characterized by means of FTIR, 1H NMR and elemental analyses. The results indicate that the average degree of alkyl group substitution on the chitosan increases with decreasing the molecular weight of the chitosan. The fully N,N-dilaurylated chitosan was found to be dissolvable in chloroform. The collapsed pressures of the samples derived from chitosan with 3000, 5000 and 10000 dalton are 47.6, 48.2 and 51.0 mN/m, respectively. The surface area occupied by the monomer unit(glucosamine) of all those samples is 0.6 nm2.展开更多
The promoter region (BCSP666) of b-conglycinin a-subunit gene from the genomic DNA of soybean Jilin 43 was isolatedby PCR method. Sequencing analysis showed that the cloned fragment BCSP666 had the similar structure ...The promoter region (BCSP666) of b-conglycinin a-subunit gene from the genomic DNA of soybean Jilin 43 was isolatedby PCR method. Sequencing analysis showed that the cloned fragment BCSP666 had the similar structure to the soybeanseed-specific promoter b-conglycinin a'-subunit gene promoter and b-conglycinin b-subunit gene promoter, and it alsocontains many motifs that contribute to the seed-specific promoter activity. Based on this sequencing analysis, wededuced that promoter fragment BCSP666 had the seed-sepecific promoter activity. And then we constructed the seed-specific expression vector pBMI666 with the promoter fragment BCSP666 and D6-fatty acid desaturase gene fromMortierella isabellina. The D6-fatty acid desaturase is the rate-limiting enzyme of the desaturation of linoleic acid in theproduction of a human essential fatty acid, g-linolenic acid(GLA). The production of g-linolenic acid(GLA) was observedin soybean callus cells, which were transformed with this vector. This confirmed the activity of the activity fragmentBCSP666.展开更多
基金Supported by the National Natural Science Foundation of China(No.2 0 2 74 0 13) ,Key Project of the Fujian ProvincialScience and Technology(No.2 0 0 2 I0 0 4 ) and the Fujian Provincial Science Foundation of China(No.E0 110 0 2 7and C0 310 0 0 9)
文摘With sodium dodecyl sulfonate(SDS) as the phase transferring catalyst, N,N-Dilauryl chitosans with a high degree of alkyl group substitution were prepared and characterized by means of FTIR, 1H NMR and elemental analyses. The results indicate that the average degree of alkyl group substitution on the chitosan increases with decreasing the molecular weight of the chitosan. The fully N,N-dilaurylated chitosan was found to be dissolvable in chloroform. The collapsed pressures of the samples derived from chitosan with 3000, 5000 and 10000 dalton are 47.6, 48.2 and 51.0 mN/m, respectively. The surface area occupied by the monomer unit(glucosamine) of all those samples is 0.6 nm2.
基金supported by the National Natural Science Foundation of China(30200176)the Key Foundation of Tianjin of China(013802511)
文摘The promoter region (BCSP666) of b-conglycinin a-subunit gene from the genomic DNA of soybean Jilin 43 was isolatedby PCR method. Sequencing analysis showed that the cloned fragment BCSP666 had the similar structure to the soybeanseed-specific promoter b-conglycinin a'-subunit gene promoter and b-conglycinin b-subunit gene promoter, and it alsocontains many motifs that contribute to the seed-specific promoter activity. Based on this sequencing analysis, wededuced that promoter fragment BCSP666 had the seed-sepecific promoter activity. And then we constructed the seed-specific expression vector pBMI666 with the promoter fragment BCSP666 and D6-fatty acid desaturase gene fromMortierella isabellina. The D6-fatty acid desaturase is the rate-limiting enzyme of the desaturation of linoleic acid in theproduction of a human essential fatty acid, g-linolenic acid(GLA). The production of g-linolenic acid(GLA) was observedin soybean callus cells, which were transformed with this vector. This confirmed the activity of the activity fragmentBCSP666.
