[ Objective] TO study the effects of microbial fermentation feeds on growth performance of piglets, and provide the basis for their promotion and application, [ Method ] All of 60 Duroc x Landrace x Yorkshire crossbre...[ Objective] TO study the effects of microbial fermentation feeds on growth performance of piglets, and provide the basis for their promotion and application, [ Method ] All of 60 Duroc x Landrace x Yorkshire crossbred weaned piglets, weighing 8-10 kg, were randomly housed in six groups, 10 piglets in each group. The piglets in control groups were fed with traditional daily food which contained aureomycin at 70 mg/kg . BW and concentrate feeds without antibiotics. While the piglets in experimental groups were fed with concentrate feeds without antibiotics and microbial fermentation feeds. [ Result] The results indicated that the average daily gain of experimental groups raised by 5.56%, and the feed conversion ratio reduced by 3.53% compared to control group. Moreover, the number of probiotics in the feces of experimental groups increased and pH value decreased significantly compared with the control groups （P 〈 0.05）. [ Conclusion] Microbial fermentation feeds can be used to replace antibiotics feeds, and promote healthy growth of the piglets.展开更多
【背景】粗糙脉孢菌LY03是从武平传统“红菌豆腐”中分离得到的主要发酵菌株。【目的】研究粗糙脉孢菌LY03菌株的基因组信息,揭示武平传统“红菌豆腐”发酵特性。【方法】采用形态学观察、ITS鉴定、重测序及框架图测序对所分离的LY03菌...【背景】粗糙脉孢菌LY03是从武平传统“红菌豆腐”中分离得到的主要发酵菌株。【目的】研究粗糙脉孢菌LY03菌株的基因组信息,揭示武平传统“红菌豆腐”发酵特性。【方法】采用形态学观察、ITS鉴定、重测序及框架图测序对所分离的LY03菌株进行鉴定和基因组信息解析。【结果】武平传统“红菌豆腐”中分离得到的主要发酵菌株LY03确定为粗糙脉孢菌,将其在中国普通微生物菌种保藏管理中心进行专利保藏,保藏号为CGMCC 3.19233。LY03菌株比对到参考基因组上的总Read数目为95.85%,测序对应深度的位点占全基因组76.13%;各变异类型在内含子区域均无变异,主要变异存在于基因组的外显子区域,具体变异数量为:单核苷酸多态性位点(single nucleotide polymorphism,SNP)位点变异总数203128个、插入缺失(insertion/deletion,InDel)突变总和26859个、拷贝数变异(copy number variation,CNV)增加和减少的拷贝总数1039个、结构变异(structure variation,SV)注释的变异总数777个;LY03菌株基因组序列长度为30538737 bp、(G+C)mol%为52.24%、编码5550个基因、编码基因占比24.3%,参与了氨基酸代谢(amino acid metabolism)、碳水化合物代谢(carbohydrate metabolism)、能量产生与转化(energy production and conversion)等多种物质的代谢途径转化过程。【结论】对武平传统“红菌豆腐”主要发酵菌株的鉴定及基因组信息分析有利于揭示其产品发酵的特性及其发酵菌株遗传信息的本质,并为将来产品发酵性能的提高及推广提供理论依据。展开更多
基金funding from Preventive Veterinary Medicine and Biotechnology,Fujian Province Key Laboratory of Colleges and Universities (Longyan College) Open-ended Fund
文摘[ Objective] TO study the effects of microbial fermentation feeds on growth performance of piglets, and provide the basis for their promotion and application, [ Method ] All of 60 Duroc x Landrace x Yorkshire crossbred weaned piglets, weighing 8-10 kg, were randomly housed in six groups, 10 piglets in each group. The piglets in control groups were fed with traditional daily food which contained aureomycin at 70 mg/kg . BW and concentrate feeds without antibiotics. While the piglets in experimental groups were fed with concentrate feeds without antibiotics and microbial fermentation feeds. [ Result] The results indicated that the average daily gain of experimental groups raised by 5.56%, and the feed conversion ratio reduced by 3.53% compared to control group. Moreover, the number of probiotics in the feces of experimental groups increased and pH value decreased significantly compared with the control groups （P 〈 0.05）. [ Conclusion] Microbial fermentation feeds can be used to replace antibiotics feeds, and promote healthy growth of the piglets.
文摘【背景】粗糙脉孢菌LY03是从武平传统“红菌豆腐”中分离得到的主要发酵菌株。【目的】研究粗糙脉孢菌LY03菌株的基因组信息,揭示武平传统“红菌豆腐”发酵特性。【方法】采用形态学观察、ITS鉴定、重测序及框架图测序对所分离的LY03菌株进行鉴定和基因组信息解析。【结果】武平传统“红菌豆腐”中分离得到的主要发酵菌株LY03确定为粗糙脉孢菌,将其在中国普通微生物菌种保藏管理中心进行专利保藏,保藏号为CGMCC 3.19233。LY03菌株比对到参考基因组上的总Read数目为95.85%,测序对应深度的位点占全基因组76.13%;各变异类型在内含子区域均无变异,主要变异存在于基因组的外显子区域,具体变异数量为:单核苷酸多态性位点(single nucleotide polymorphism,SNP)位点变异总数203128个、插入缺失(insertion/deletion,InDel)突变总和26859个、拷贝数变异(copy number variation,CNV)增加和减少的拷贝总数1039个、结构变异(structure variation,SV)注释的变异总数777个;LY03菌株基因组序列长度为30538737 bp、(G+C)mol%为52.24%、编码5550个基因、编码基因占比24.3%,参与了氨基酸代谢(amino acid metabolism)、碳水化合物代谢(carbohydrate metabolism)、能量产生与转化(energy production and conversion)等多种物质的代谢途径转化过程。【结论】对武平传统“红菌豆腐”主要发酵菌株的鉴定及基因组信息分析有利于揭示其产品发酵的特性及其发酵菌株遗传信息的本质,并为将来产品发酵性能的提高及推广提供理论依据。
