The rice blast,caused by fungus Magnaporthe oryzae,is a major constraint to the world food security.Hyphal growth is the foundation of fungal development and proliferation of fungi.To investigate genes involved in hyp...The rice blast,caused by fungus Magnaporthe oryzae,is a major constraint to the world food security.Hyphal growth is the foundation of fungal development and proliferation of fungi.To investigate genes involved in hyphal growth of this fungus,digital gene expression tag profiling was used to compare a previously generated temperature-sensitive mutant which defect at hyphae growth and reduction on pathogenicity,with its related wildtype strain.416 genes were detected as differential expression,178 of which were specifically expressed in Guy-11 but down-regulated expression in the mutant.Functional classification analysis revealed the phenotype mutation may be mainly caused by a defection in translational and vacuole- related processes.The results and the protocol used will improve our knowledge on morphogenesis and promote the further study on M.oryzae pathogenesis.展开更多
The promoter of NAR gene in Magnaporthe grisea was isolated and sequenced. The promoter sequences contained the "TATA" box, the "CAAT" box, and binding sites for fungal regulatory proteins. Program...The promoter of NAR gene in Magnaporthe grisea was isolated and sequenced. The promoter sequences contained the "TATA" box, the "CAAT" box, and binding sites for fungal regulatory proteins. Programs that predict promoter sequences in-dicated that promoter sequence lies between locations 430 and 857 of the NAR promoter fragment. GFP expression under the NAR promoter and NAR transcript analysis revealed that this promoter is activated primarily at the mycelial stage in the rice blast fungus and could be used to express native or extrinsic genes in the mycelia of the rice blast fungus.展开更多
Magnaporthe oryzae, a filamentous ascomycete fungus, is well known as the causal agent of rice blast. With the technology of suppression subtractive hybridization （SSH）, it was previously found that MGG_06001 （or n...Magnaporthe oryzae, a filamentous ascomycete fungus, is well known as the causal agent of rice blast. With the technology of suppression subtractive hybridization （SSH）, it was previously found that MGG_06001 （or named MoNEM1）, a gene of M. oryzae homologous to the NEM1 （nuclear envelope morphology protein 1） gene of baker＇s yeast （Saccharomyces cerevisiae）, is differentially expressed between the mature appressium and the conidium and mycelium. This study aimed to characterize the function of MoNEM1 gene by knocking it out using the method of target gene replacement. The AMoneml mutants exhibited reduced mycelial growth and conidiation. However, disruption of MoNEM1 gene does not affect the pathogenicity of M. oryzae on barley and rice.展开更多
Atoms in the coherent superposition state prepared by a pulse pair are used as a novel optical memory material where a single interrogation pulse will produce a new pulse pair preserving the relative amplitudes and ph...Atoms in the coherent superposition state prepared by a pulse pair are used as a novel optical memory material where a single interrogation pulse will produce a new pulse pair preserving the relative amplitudes and phases of the preparing pulse pair. Such a coherent superposition state can also be specially tailored along the propagation path to generate Raman scattering in a relatively short distance with very high efficiency.展开更多
We report here some new anomalous pulse optogalvanic signals arising from transitions between different levels of metal atoms and suggest a possible mechanism to explain this phenomenon.
The quantum dynamics of a three-level Vee-system can be controlled by using two single-mode cw lasers.The dc component of the probability for finding a specific state in laser fields can be obtained by matching the in...The quantum dynamics of a three-level Vee-system can be controlled by using two single-mode cw lasers.The dc component of the probability for finding a specific state in laser fields can be obtained by matching the intensities,phases and detunings of the lasers to relevant eigenvalues of the quantum system.The selectivity of control does not depend strongly on the laser intensities but the absolute amount of the product does strongly.There are generally three paths to reach such a dc component control and,at some points,two of them cross each other causing a near 100%control.The stability of the process to the fluctuation of the experiment was analyzed.展开更多
基金supported by the Natural Science Foundation of Zhejiang Province,China(Y3110028 and LQ12C14003)
文摘The rice blast,caused by fungus Magnaporthe oryzae,is a major constraint to the world food security.Hyphal growth is the foundation of fungal development and proliferation of fungi.To investigate genes involved in hyphal growth of this fungus,digital gene expression tag profiling was used to compare a previously generated temperature-sensitive mutant which defect at hyphae growth and reduction on pathogenicity,with its related wildtype strain.416 genes were detected as differential expression,178 of which were specifically expressed in Guy-11 but down-regulated expression in the mutant.Functional classification analysis revealed the phenotype mutation may be mainly caused by a defection in translational and vacuole- related processes.The results and the protocol used will improve our knowledge on morphogenesis and promote the further study on M.oryzae pathogenesis.
基金the National Natural Science Foundation of China (Nos. 30270049 and 30470064)the Natural Science Foundation of Zhejiang Province, China (No. Y304211)
文摘The promoter of NAR gene in Magnaporthe grisea was isolated and sequenced. The promoter sequences contained the "TATA" box, the "CAAT" box, and binding sites for fungal regulatory proteins. Programs that predict promoter sequences in-dicated that promoter sequence lies between locations 430 and 857 of the NAR promoter fragment. GFP expression under the NAR promoter and NAR transcript analysis revealed that this promoter is activated primarily at the mycelial stage in the rice blast fungus and could be used to express native or extrinsic genes in the mycelia of the rice blast fungus.
基金supported by the National Natural Science Foundation of China (30671123 and 31000077)
文摘Magnaporthe oryzae, a filamentous ascomycete fungus, is well known as the causal agent of rice blast. With the technology of suppression subtractive hybridization （SSH）, it was previously found that MGG_06001 （or named MoNEM1）, a gene of M. oryzae homologous to the NEM1 （nuclear envelope morphology protein 1） gene of baker＇s yeast （Saccharomyces cerevisiae）, is differentially expressed between the mature appressium and the conidium and mycelium. This study aimed to characterize the function of MoNEM1 gene by knocking it out using the method of target gene replacement. The AMoneml mutants exhibited reduced mycelial growth and conidiation. However, disruption of MoNEM1 gene does not affect the pathogenicity of M. oryzae on barley and rice.
文摘Atoms in the coherent superposition state prepared by a pulse pair are used as a novel optical memory material where a single interrogation pulse will produce a new pulse pair preserving the relative amplitudes and phases of the preparing pulse pair. Such a coherent superposition state can also be specially tailored along the propagation path to generate Raman scattering in a relatively short distance with very high efficiency.
文摘We report here some new anomalous pulse optogalvanic signals arising from transitions between different levels of metal atoms and suggest a possible mechanism to explain this phenomenon.
文摘The quantum dynamics of a three-level Vee-system can be controlled by using two single-mode cw lasers.The dc component of the probability for finding a specific state in laser fields can be obtained by matching the intensities,phases and detunings of the lasers to relevant eigenvalues of the quantum system.The selectivity of control does not depend strongly on the laser intensities but the absolute amount of the product does strongly.There are generally three paths to reach such a dc component control and,at some points,two of them cross each other causing a near 100%control.The stability of the process to the fluctuation of the experiment was analyzed.
