Zyxin promotes hepatocellular carcinoma progression via the activation of AKT/mTOR signaling pathway

Hepatocellular carcinoma(HCC)is a common malignancy that is driven by multiple genes and pathways.The aim of this study was to investigate the role and specific mechanism of the actin-interacting protein zyxin(ZYX)in HCC.We found that the expression of ZYX was significantly higher in HCC tissues compared to that in normal liver tissues.In addition,overexpression of ZYX in hepatoma cell lines(PLC/PRF/5,HCCLM3)enhanced their proliferation,migration and invasion,whereas ZYX knockdown had the opposite effects(SK HEP-1,Huh-7).Furthermore,the change in the expression levels of ZYX also altered that of proteins related to cell cycle,migration and invasion.Similar results were obtained with xenograft models.The AKT/mTOR signaling pathway is one of the key mediators of cancer development.While ZYX overexpression upregulated the levels of phosphorylated AKT/mTOR proteins,its knockdown had the opposite effect.In addition,the AKT inhibitor MK2206 neutralized the pro-oncogenic effects of ZYX on the HCC cells,whereas the AKT activator SC79 restored the proliferation,migration and invasion of HCC cells with ZYX knockdown.Taken together,ZYX promotes the malignant progression of HCC by activating AKT/mTOR signaling pathway,and is a potential therapeutic target in HCC.

A Deep-Learning and Transfer-Learning Hybrid Aerosol Retrieval Algorithm for FY4-AGRI:Development and Verification over Asia

The Advanced Geosynchronous Radiation Imager(AGRI)is a mission-critical instrument for the Fengyun series of satellites.AGRI acquires full-disk images every 15 min and views East Asia every 5 min through 14 spectral bands,enabling the detection of highly variable aerosol optical depth(AOD).Quantitative retrieval of AOD has hitherto been challenging,especially over land.In this study,an AOD retrieval algorithm is proposed that combines deep learning and transfer learning.The algorithm uses core concepts from both the Dark Target(DT)and Deep Blue(DB)algorithms to select features for the machinelearning(ML)algorithm,allowing for AOD retrieval at 550 nm over both dark and bright surfaces.The algorithm consists of two steps:①A baseline deep neural network(DNN)with skip connections is developed using 10 min Advanced Himawari Imager(AHI)AODs as the target variable,and②sunphotometer AODs from 89 ground-based stations are used to fine-tune the DNN parameters.Out-of-station validation shows that the retrieved AOD attains high accuracy,characterized by a coefficient of determination(R2)of 0.70,a mean bias error(MBE)of 0.03,and a percentage of data within the expected error(EE)of 70.7%.A sensitivity study reveals that the top-of-atmosphere reflectance at 650 and 470 nm,as well as the surface reflectance at 650 nm,are the two largest sources of uncertainty impacting the retrieval.In a case study of monitoring an extreme aerosol event,the AGRI AOD is found to be able to capture the detailed temporal evolution of the event.This work demonstrates the superiority of the transfer-learning technique in satellite AOD retrievals and the applicability of the retrieved AGRI AOD in monitoring extreme pollution events.

Device design principles and bioelectronic applications for flexible organic electrochemical transistors

Organic electrochemical transistors(OECTs) exhibit significant potential for applications in healthcare and human-machine interfaces, due to their tunable synthesis, facile deposition, and excellent biocompatibility. Expanding OECTs to the fexible devices will significantly facilitate stable contact with the skin and enable more possible bioelectronic applications. In this work,we summarize the device physics of fexible OECTs, aiming to offer a foundational understanding and guidelines for material selection and device architecture. Particular attention is paid to the advanced manufacturing approaches, including photolithography and printing techniques, which establish a robust foundation for the commercialization and large-scale fabrication. And abundantly demonstrated examples ranging from biosensors, artificial synapses/neurons, to bioinspired nervous systems are summarized to highlight the considerable prospects of smart healthcare. In the end, the challenges and opportunities are proposed for fexible OECTs. The purpose of this review is not only to elaborate on the basic design principles of fexible OECTs, but also to act as a roadmap for further exploration of wearable OECTs in advanced bio-applications.

适用于城市轨道交通车辆的可维修性定量分析方法研究

目前,可靠性、可用性、可维修性、安全性的相关研究技术已广泛应用于城市轨道交通车辆全生命周期的各个阶段,针对可靠性与安全性的研究相对较多,而针对可维修性的研究较少,因此可用性研究工作受到了影响。以产品运营和维护为例,虽然有一些城市轨道交通运营公司已开展针对可维修性定性分析的研究工作,但针对可维修性定量分析研究工作却开展较少,主要原因是不明确可维修性定量分析需要研究哪些指标,研究结论对实际工作有何帮助。因此,文章提出了适用于城市轨道交通车辆的可维修性定量分析方法,阐述了样本收集要求、计算分析过程,并给出了研究成果在实际运营维护阶段的具体用途,可帮助城市轨道交通运营公司解决一些实际问题。

损伤开裂承压壳体泄漏率分析模型改进与验证

承压壳体发生放射性、易燃等危险气体泄漏会对环境造成重大威胁,泄漏状况评估是应急措施优化的必要前提。针对严重事故下的厚壳壁微开裂,建立了一套快速定量的泄漏率分析数值模型。基于流量守恒原理,提出了厚壁结构中多段微裂缝构成变截面通道的泄漏率计算改进模型;采用混凝土塑性损伤模型模拟极限载荷下结构的非线性破坏,通过等参函数实现几何不规则单元与规则空间之间的变换,改进了弥散型微裂缝扩展尺度的计算模型。通过数值算例对比文献中的结果,分别验证了以上两个子模型的稳定性和有效性,并由受损剪力墙的泄漏率分析进一步验证了模型的可行性。最后,将模型应用于抗高温内压损伤过程中的某预应力混凝土复杂承压壳体,结果表明:改进的模型适用于厚壁、带孔道等复杂构造承压结构的泄漏率分析,具有一定的实际工程意义。

BaTiO_(3)/Polyurethane Dielectric Composites with Diels-Alder Bond for Improved Self-Healing Properties

In general,self-healing dielectric composites are mainly composed of polar hydrogen bonds,which have high hydrophilicity and are unsuitable for humid environment.Dielectric composite with Diels-Alder(D-A)bond contains covalent bonds,it can be adopted as an efficient self-healing material.Here,we construct self-healing barium titanate(BT)/polyurethane(PU)dielectric composites by adopting PU with D-A bond as matrix(BT/PU-DA).The prepared 10%BT/PU-DA composite exhibits superior self-healing ability than that of PUDA.Moreover,its dielectric constant can reach 9.3 with a loss of only 0.04 at 1000 Hz and maintain 93%repair efficiency of tensile strength.The experimental analysis suggests the introduction of D-A bond can enhance the thermostability and self-healing ability of BT/PU-DA composite.In addition,the incorporation of BT nanoparticles and D-A bond in the self-healing composite contributes to the lower dielectric loss and excellent tensile strength after healing.The adopted strategy is a promising and facile approach to develop highly efficient selfhealing dielectric material,which will be conducive to reuse and sustainable development of the electronic packaging material in aqueous medium or wet environment.

Optimal Micro⁃Task Scheduling for Multi⁃Hop D2D⁃Enabled Mobile Edge Computing

Mobile Edge Computing(MEC)is a promising solution to tackle the upcoming computing tsunami in 5G/6G era by effectively utilizing the idle resource at the mobile edge.In this work,a multi⁃hop D2D⁃enabled MEC scenario was studied,where mobile devices at network edge connect and share resources with each other via multi⁃hop D2D.The research focus was on the micro⁃task scheduling problem in the multi⁃hop D2D⁃enabled MEC system,where each task was divided into multiple sequential micro⁃tasks,such as data downloading micro⁃task,data processing micro⁃task,and data uploading micro⁃task,according to their functionalities as well as resource requirements.A joint Task Failure Probability and Energy Consumption Minimization(TFP⁃ECM)problem was proposed,aiming at minimizing the task failure probability and the energy consumption jointly.To solve the problem,several linearization methods were proposed to relax the constraints and convert the original problem into an integer linear programming(ILP).Simulation results show that the proposed solution outperformed the existing solutions(with indivisible tasks or without resource sharing)in terms of both total cost and task failure probability.

移动荷载下各向异性层状地基-柔性路面结构动力响应的半解析解

提出了移动荷载作用下各向异性层状地基上柔性路面结构动力响应分析的半解析解法。基于土-结构相互作用理论,建立了柔性路面-层状地基的动力耦合模型。对近场道路结构建立波数域有限元模型,基于精细积分及谱元法原理建立远场地基的谱元模型,利用子结构法来确定近-远场耦合界面处的边界条件,进而实现近场和远场结构的耦合,利用快速Fourier变换获得柔性路面在时空域的动力响应解答。该文方法既可以考虑道路结构的几何特性,也可以计入地基层状分布及各向异性特性的影响。近场有限域模型尺寸不会受到地基层数及层厚的影响,数值求解稳定。通过与解析解的对比,验证了提出方法的有效性与合理性。数值算例对比分析了道路-地基耦合模型与以往整体层状道路模型所得动力响应的差异,并讨论了道路结构层弹性模量的影响规律。

Clinical Observation of 48 Cases of Black Eye Circle Treated with Modified Yu Rong San Gel Eye Mask

Objective:To observe the clinical effect of modified Yu Rong San gel eye mask in the treatment of dark circles.Methods:96 cases of patients with dark circles were randomly divided into treatment group and control group.In the control group and the treatment group,the common eye mask and the modified Yu Rong San gel eye mask were applied to the black circle area respectively.After 15min,water was used to wash the face,the application of the mask for one time a day,four weeks,one course,and three courses were observed.Results:After three courses of treatment,the total effective rate of the treatment group was higher than that of the control group(P<0.05);the area of black eye circles in the treatment group was smaller than that before treatment(P<0.05);compared with the control group,the score of black eye circles was lower than that before treatment,the difference was statistically significant(P<0.05).Conclusions Addition and reduction of Yu Rong San gel eye mask is effective in the treatment of dark circles.

面向水下机器人的多模态人机交互控制

近年来水下机器人在海洋科学研究、海洋工程及战略高技术等领域得到了广泛应用,但由于水下操作指令的繁杂,一般需要多人协同操作且交互控制效率较低。针对此问题,该文提出了一种面向水下机器人的多模人机交互控制方案,通过上肢可穿戴外骨骼和Myo手环检测操作者手臂和手部的运动状态,实现对水下机器人的运动控制。利用机器学习算法和检测手臂关节运动角度的方式,完成对肌电信号和肢体动作的控制意图识别。通过网络将肢体动作和肌电信号的控制指令发送到水下虚拟环境中,实现多模态融合的控制效果,并完成了18种控制指令的映射。最后,选取4位操作者进行水下机器人的人机交互控制测试,实验结果表明了多模人机交互控制方式在水下作业中的可行性。

头孢克肟颗粒溶出度测定方法建立和体外溶出曲线一致性评价

目的建立快速测定头孢克肟颗粒溶出度的HPLC法,评价自研制剂和参比制剂的体外溶出曲线的相似性。方法采用HPLC法,色谱柱:Thermo Accucore C18柱(50 mm×4.6 mm,2.6μm);流动相:0.8%三氟乙酸水溶液-乙腈(80∶20);流速:1.2 mL·min^(-1);检测波长:254 nm;柱温:45℃;进样量:5μL。分别测定头孢克肟颗粒自研制剂和参比制剂在4种溶出介质(水、pH 1.2盐酸溶液、pH 6.8磷酸盐缓冲液和pH 7.5磷酸盐缓冲液)中的溶出曲线。结果方法学验证结果均符合检测要求。头孢克肟在10.05~251.30μg·mL^(-1)范围内线性关系良好,在4种介质中的平均回收率分别为99.3%,99.1%,100.2%和99.8%,RSD分别为0.79%,0.74%,0.48%和0.45%(n=9)。自研制剂和参比制剂在水和pH 1.2盐酸溶液中的溶出度相似因子分别为57和55,在pH 6.8磷酸盐缓冲液和pH 7.5磷酸盐缓冲液中15 min的溶出度均大于85%。结论本方法快速、准确,可用于头孢克肟颗粒溶出度的测定,为头孢克肟颗粒的体外溶出曲线一致性评价提供参考。

Design of Smart Home System Based on ZigBee Technology and R&D for Application

In this paper, a smart home system based on ZigBee technology is designed. The system includes home network, home server and mobile terminal. The program is highly scalable and cost-effective. This paper developed the home server-side application based on MFC technology and the mobile terminal application. The mobile client can remotely control home devices and query the running state, electric energy information and historical data of home devices. At the same time, the home server-side application can store electric energy information and electricity consumption of home devices. Combined with household distributed photovoltaic generation system, the system can be applied to home energy management system. Through running tests and application, the results show that the system has realized basic functions of smart home and achieved the desired design goals.

Hypertriglyceridemia and acute pancreatitis:clinical and basic research—a narrative review

Hypertriglyceridemia(HTG)is the third most common cause of acute pancreatitis(AP),and the incidence of HTG-induced AP(HTG-AP)is rising worldwide as a result of changes in lifestyle and dietary habits.In China,HTG accounts for 10%to 20%of the causes of AP,even becoming the second leading cause of AP.The presentation and diagnosis of HTG-AP is similar to that of AP from other causes,however,the complication rates and severe AP are significantly higher in patients with HTG-AP than in patients with other etiologies.Regarding the management of HTG-AP,the initial management is similar to that of AP from other causes and mainly includes fluid resuscitation,pain control,and nutritional support.In addition to supportive care,it is necessary to take appropriate measures to decrease serum TG levels,in order to alleviate the progression of AP and prevent recurrence.This review aims to summarize existing clinical and basic research evidence of HTG-AP and seeks to highlight the epidemiology,definition,pathogenesis,clinical course,diagnosis,management,prevention of recurrence,and specific clinical scenarios,such as HTG-AP during pregnancy,HTG-AP with diabetic ketoacidosis.

横观各向同性层状地基动应力响应的求解与分析

动力响应问题的求解对于地基在外荷载作用下引起的弹性波动问题研究有重要的意义。本文提出了一种求解横观各向同性层状地基在施加时间简谐荷载作用下任意点的应力响应的算法。此算法利用傅里叶变换将广义平面应变问题频率-空间域的动力方程转化到频率-波数域内,结合对偶变量的引入,利用高精度的精细积分算法对状态方程进行求解,在得到频率-波数域内的位移响应的基础上,利用傅里叶逆变换得到任意点的动应力响应。简谐荷载不仅可以施加在地基表面,而且可以施加在地基内部。对比算例验证了本文算法的准确性,同时对地基各向异性特性、激励频率和阻尼比对动应力响应的影响进行了参数分析,为工程实际提供可靠的数值依据。

热弹性动力学耦合问题的插值型移动最小二乘无网格法研究

该文基于插值型移动最小二乘法,将无网格局部Petrov-Galerkin(MLPG)法用于二维耦合热弹性动力学问题的求解。修正的Fourier热传导方程和弹性动力控制方程通过加权余量法来离散,Heaviside分段函数作为局部弱形式的权函数,从而得到描述热耦合问题的二阶常微分方程组。然后利用微分代数方法,温度和位移作为辅助变量,将上述二阶常微分方程组转换成常微分代数系统,采用Newmark逐步积分法进行求解。该方法无需Laplace变换可直接得到温度场和位移场数值结果,同时插值型移动最小二乘法构造的形函数由于满足Kronecker delta特性,因此能直接施加本质边界条件。最后通过两个数值算例来验证该方法的有效性。

复杂地基条件下土-结构动力相互作用分析

结构和地基动力相互作用(SSI)分析对大坝、桥梁、核电、地下结构的抗震安全评价具有重要意义。本文对SSI分析的发展现状和工程设计实践进行了扼要评述。值得指出,2011年日本福岛核电事故后,国际上普遍提高了对核电结构抗震安全性的需求。因此,对重要结构的安全评价发展更为完善的先进SSI计算模型势在必行。为此目的,对处于复杂地基条件下的SSI分析提出一种新的计算模型,可以方便地考虑结构近场各种非均质因素的影响而不增加计算工作量,其中包括地基分层、基础埋深、建基面水平、存在相邻建筑物、附近出现软弱地质体等因素。这使SSI的计算更为准确和高效。论文的数值算例论证了所建议方法的有效性。

Effect of Viral Antigen Levels on the Serological Response and Efficiency of the Binary Ethylenimine-Inactivated Bluetongue Virus Serotype-16 Vaccine

Bluetongue (BT) is a serious hemorrhagic disease of ruminants caused by bluetongue virus (BTV). Inactive BTV vaccines have been successful in field trials in some areas, and inactivated vaccines are considered safer. However, information about the effect of the viral antigen level on the serological response and efficiency of the inactive BTV-16 vaccine is lacking. In the present study, the serological response and efficiency of the viral antigen concentration in the binary ethylenimine-inactivated Chinese BTV serotype-16 vaccine were investigated. The viral antigens in the viral suspension (VS) were quantified using a modified BTV AC-ELISA method. Four batches of vaccine containing 1, 5, 10, and 50 μg/ml of viral antigen were generated from the VS. Four groups of naive Chinese sheep were vaccinated with the different vaccine batches, and the serological response and vaccine efficiency were investigated before and after challenge infection. The vaccines containing 10 and 50 μg/ml of viral antigen induced significant ELISA and neutralizing antibody titers 14 days after vaccination, whereas the vaccines containing 1 and 5 μg/ml of viral antigen did not have these effects. A booster immunization at 21 days enhanced all groups’ antibody titers;however, the increased titer was related to the viral antigen level. In contrast to the serological response, the viral antigen level of the vaccines did not have a significant effect on the vaccine efficiency. With the exception of one sheep from the 5 μg/ml viral antigen group, all vaccinated sheep from the four antigen level groups showed strong resistance to infection based on their clinical symptoms, rectal temperatures and viremia. Collectively, these data suggested that viral antigen levels from 1 to 50 μg/ml had a significant effect on the serological response of the animals but a limited effect on the vaccine efficiency. The BTV-16 vaccine containing 1 μg/ml of viral antigen was sufficient to achieve high efficiency, but only the vaccines with more than 10 μg/ml of antigen induced a significant antibody response. To obtain a better serological response, we suggest the use of vaccines with more than 10 μg/ml of viral antigen. The findings in the study will be useful for BTV vaccine production.

饱和层状地基条形基础动刚度的精细积分算法

采用基于积分变换、对偶方程和精细积分的算法求解饱和多层地基条形基础的动刚度问题。利用Fourier积分变换得到Biot多孔饱和介质波动方程在频域-波数域内的常微分方程,引入广义对偶变量,对二阶常微分波动方程进行降阶处理;针对地表自由排水和封闭排水边界条件,给出了适于精细积分算法的矩阵表达;对控制方程采用精细积分解答,得到波数域内的格林函数,对结果进行逆Fourier积分变换,得到空间域的力和位移关系;施加边界条件,最终得到刚性条形基础的动刚度的解答。通过与文献已有结果进行对比,验证了精细积分算法的准确性。最后计算两组算例,分别分析了表面薄层的孔隙率、渗透力阻尼系数对条形基础动刚度的影响。

Protective effect of borneol combined with safflower on neurovascular unit in rats with ischemic stroke

Background:Compatibility is a characteristic of the clinical application of traditional Chinese medicine,often leading to enhanced therapeutic effects.In the treatment of cerebral ischemia,blood-activating and open orifices herbs are frequently used individually;however,their combination is not commonly practiced.This study aims to investigate the impact of combining safflower and borneol as examples of open orifices herbs and blood-activating herbs on the neurovascular unit in rats with ischemic stroke.The objective is to determine whether this combination exhibits superior therapeutic efficacy compared to using borneol or safflower alone while exploring its underlying mechanism.These findings may provide novel insights for clinical treatments.Methods:SD male rats were randomly divided into 6 groups:sham operation group,model group,borneol group(0.1 g/kg),safflower group(5 g/kg),borneol combined with safflower group(0.1 g/kg+5 g/kg)and nimodipine group(0.01 g/kg).The middle cerebral artery cerebral ischemia(MCAO)model were prepared after continuous intragastric administration for 7 days in each group,the neurological function of each group were scored 24h after operation,and water content in brain tissue were measured by weighing method.The activity of superoxide dismutase(SOD)and the contents of nitric oxide(NO)and malondialdehyde(MDA)in brain tissue and serum were determined by spectrophotometry,and the mRNA expressions of matrix metalloproteinase 2(MMP-2),tight junction protein 1(ZO-1),vascular endothelial growth factor(VEGF)and brain-derived neurotrophic factor(BDNF)were detected by Real time PCR.Result:Compared with the model group,the group treated with borneol combined with safflower exhibited a significant decrease in the neural function score of MCAO rats(P<0.01).Additionally,it led to a reduction in brain tissue water content(P<0.01),elevated SOD activity,and reduced levels of NO and MDA in both serum and brain tissue(P<0.01 or P<0.05).Moreover,this treatment resulted in a decrease in the mRNA expression of MMP-2 and an increase in ZO-1 in brain tissue,along with an increase in the mRNA expression of VEGF and BDNF(P<0.01).Conclusion:Borneol combined with safflower demonstrates a protective effect on the neurovascular unit in rats with ischemic stroke.This effect is likely associated with increased SOD activity,reduced MDA and NO content in both serum and brain tissue of MCAO rats,and a decrease in MMP-2 mRNA expression in brain tissue,coupled with an increase in ZO-1,VEGF,and BDNF mRNA expression.These effects were superior to those observed with borneol or safflower administered alone.