In order to study the distribution of shale gas reservoir in the Babaoshan Basin of Eastern Kunlun,the wide-field electromagnetic(WFEM)survey was carried out to obtain the spatial distribution characteristics of the u...In order to study the distribution of shale gas reservoir in the Babaoshan Basin of Eastern Kunlun,the wide-field electromagnetic(WFEM)survey was carried out to obtain the spatial distribution characteristics of the underground electrical volume resistivity based on the delineation of the scope of the Babaoshan Basin by regional gravity data.The basic characteristics of the basement,basin framework,and extension,vertical change,burial depth of dark mud shale in this area were identified,and the electrical distribution of the Babaoshan mud shale horizon was revealed,which has been proved to be a good geological effect by drilling.The exploration results show that the WFEM has significant effects on the exploration of shale gas occurrence strata,which meets the needs of investigation and evaluation of multi-layered and large-scale shale gas,and plays a good demonstration role in the follow-up shale gas exploration.展开更多
目的:探究多黏菌素B酶解产物(PMBH)的制备方法及其结构确证,并初步探讨其对利奈唑胺的体外抗菌增效作用。方法:采用萃取、柱层析等步骤对多黏菌素B水解后的产物进行分离纯化,得到多黏菌素B水解产物单体,通过MS和NMR等方法对其进行结构...目的:探究多黏菌素B酶解产物(PMBH)的制备方法及其结构确证,并初步探讨其对利奈唑胺的体外抗菌增效作用。方法:采用萃取、柱层析等步骤对多黏菌素B水解后的产物进行分离纯化,得到多黏菌素B水解产物单体,通过MS和NMR等方法对其进行结构确证。采用棋盘法评价多黏菌素B母核化合物(polymyxin B hydrolysates 1,PMBH1)与利奈唑胺联用的抗铜绿假单胞菌和肺炎克雷伯菌的活性。结果:制备分离得到1种PMBH1,并结合NMR数据确证了水解产物PMBH1与文献报道的polymyxin B octapeptide(PBOP)同质。联合药敏试验显示,当利奈唑胺单用时,铜绿假单胞菌和肺炎克雷伯菌对其不敏感;而当其与0. 15μmol·mL-1浓度的PBOP联用时,增效作用最强,能使其MIC降至2. 5μg·mL-1,活性增强128倍。结论:PBOP与利奈唑胺在体外抗铜绿假单胞菌和肺炎克雷伯菌的联合药敏试验中呈协同作用。展开更多
基金Project(2019-SF-141)supported by Science and Technology Program of Qinghai Province,ChinaProjects(2017042105kc055,2017042014ky014)supported by Geological Exploration Foundation of Qinghai Province,China。
文摘In order to study the distribution of shale gas reservoir in the Babaoshan Basin of Eastern Kunlun,the wide-field electromagnetic(WFEM)survey was carried out to obtain the spatial distribution characteristics of the underground electrical volume resistivity based on the delineation of the scope of the Babaoshan Basin by regional gravity data.The basic characteristics of the basement,basin framework,and extension,vertical change,burial depth of dark mud shale in this area were identified,and the electrical distribution of the Babaoshan mud shale horizon was revealed,which has been proved to be a good geological effect by drilling.The exploration results show that the WFEM has significant effects on the exploration of shale gas occurrence strata,which meets the needs of investigation and evaluation of multi-layered and large-scale shale gas,and plays a good demonstration role in the follow-up shale gas exploration.
文摘目的:探究多黏菌素B酶解产物(PMBH)的制备方法及其结构确证,并初步探讨其对利奈唑胺的体外抗菌增效作用。方法:采用萃取、柱层析等步骤对多黏菌素B水解后的产物进行分离纯化,得到多黏菌素B水解产物单体,通过MS和NMR等方法对其进行结构确证。采用棋盘法评价多黏菌素B母核化合物(polymyxin B hydrolysates 1,PMBH1)与利奈唑胺联用的抗铜绿假单胞菌和肺炎克雷伯菌的活性。结果:制备分离得到1种PMBH1,并结合NMR数据确证了水解产物PMBH1与文献报道的polymyxin B octapeptide(PBOP)同质。联合药敏试验显示,当利奈唑胺单用时,铜绿假单胞菌和肺炎克雷伯菌对其不敏感;而当其与0. 15μmol·mL-1浓度的PBOP联用时,增效作用最强,能使其MIC降至2. 5μg·mL-1,活性增强128倍。结论:PBOP与利奈唑胺在体外抗铜绿假单胞菌和肺炎克雷伯菌的联合药敏试验中呈协同作用。
