In this paper, a new diterpene together with seven known diterpenes was isolated from Wedelia prostrata. The chemical structure of the new compound was elucidated via 1D and 2D nuclear magnetic resonance（NMR） tech- ...In this paper, a new diterpene together with seven known diterpenes was isolated from Wedelia prostrata. The chemical structure of the new compound was elucidated via 1D and 2D nuclear magnetic resonance（NMR） tech- niques and mass spectrometry and identified to be 3a-phenylpropionoyloxy-ent-kaur-16-en-oic acid（l）. The isolated diterpenes were tested for their cytotoxicity activities via 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bro- mide（MTT） assay. The results show that compounds 1, 2, 3, 4 and 6 exhibit different levels of cytotoxic activities. Especially, compound 2 shows significant cytotoxicity toward HeLa and A549 cell lines（IC50=6.14 and 8.76 μmol/L）.展开更多
基金Supported by the National Natural Science Foundation of China（No.31300285）, the Natural Science Foundation of Fujian Province, China（No.2015J01348）, the Specialized Research Fund for the Doctoral Program of Higher Education of China （No.20110073120072） and the Fujian Province Development Guidance（Key） Project, China（No.2014Y0074）.
文摘In this paper, a new diterpene together with seven known diterpenes was isolated from Wedelia prostrata. The chemical structure of the new compound was elucidated via 1D and 2D nuclear magnetic resonance（NMR） tech- niques and mass spectrometry and identified to be 3a-phenylpropionoyloxy-ent-kaur-16-en-oic acid（l）. The isolated diterpenes were tested for their cytotoxicity activities via 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bro- mide（MTT） assay. The results show that compounds 1, 2, 3, 4 and 6 exhibit different levels of cytotoxic activities. Especially, compound 2 shows significant cytotoxicity toward HeLa and A549 cell lines（IC50=6.14 and 8.76 μmol/L）.
