Atlantic salmon(Salmo salar)is an important economic fish that is seriously threatened by various viruses.A cell line designated as ASF derived from the caudal fin tissue of Atlantic salmon was established and charact...Atlantic salmon(Salmo salar)is an important economic fish that is seriously threatened by various viruses.A cell line designated as ASF derived from the caudal fin tissue of Atlantic salmon was established and characterized in this study.ASF cells grew well in Dulbecco’s modified Eagle’s medium(DMEM)containing 20%fetal bovine serum at 20℃.DNA sequencing and comparative analysis of the cytochrome B gene verified that the ASF cell line originated from Atlantic salmon.Chromosome analysis indicated that the modal chromosome number of ASF cells was 58.Viral susceptibility test showed that ASF cells were susceptive to two important fish viruses,viral hemorrhagic septicemia virus(VHSV)and red-spotted grouper nervous necrosis virus(RGNNV).Viral replication in ASF cells was further confirmed by qRT-PCR and transmission electron microscopy.Moreover,VHSV and RGNNV infections could induce the cellular responses in ASF cells,as indicated by the differential expression of cellular antiviral response-related genes,interferon-1 and Mx-1.In conclusion,the newly established ASF cell line can be applied as an in vitro tool in fish virology and immunity studies.展开更多
目的通过研究哺乳动物雷帕霉素靶蛋白(mammalian target of rapamycin,mTOR)与结直肠癌的生物信息,意在推动结直肠癌医疗手段进步,实现挽救结直肠癌患者生命的目的。方法分析2013年5月—2021年5月癌症基因组图谱(the cancer genome atla...目的通过研究哺乳动物雷帕霉素靶蛋白(mammalian target of rapamycin,mTOR)与结直肠癌的生物信息,意在推动结直肠癌医疗手段进步,实现挽救结直肠癌患者生命的目的。方法分析2013年5月—2021年5月癌症基因组图谱(the cancer genome atlas,TCGA)公共数据库的COAD&READ患者数据698份,其中正常组51例,结肿瘤组647份。分析了TCGA结直肠癌患者的表达谱、mTOR的表达和生存差异,并探讨了mTOR的共表达调控网络。此外,还探讨了mTOR与富集通路、微卫星不稳定性(microsatellite instability,MSI)、肿瘤突变负荷(tumor mutation burden,TMB)的关系。结果基于GSE73360数据库分析mTOR在组织中的表达差异,mTOR在正常组织中表达(7.0084±0.429089872)低于mTOR在肿瘤组织中表达(8.04058±0.784400233),差异有统计学意义(P<0.05)。共筛选出541个与mTOR表达显著相关的基因。核心基因高低表达在MSI和TMB方面比较,差异有统计学意义(P<0.05)。结论mTOR可以作为结直肠癌诊断和预后的生物标志物。展开更多
中国土壤流失方程(Chinese soil loss equation,CSLE)是我国用于定量评价和预测区域土壤侵蚀的重要模型。水土保持耕作措施因子(T)是该模型中的7个关键因子之一,能反映水土保持耕作措施对土壤侵蚀的削减作用,其准确估算对基于CSLE模型...中国土壤流失方程(Chinese soil loss equation,CSLE)是我国用于定量评价和预测区域土壤侵蚀的重要模型。水土保持耕作措施因子(T)是该模型中的7个关键因子之一,能反映水土保持耕作措施对土壤侵蚀的削减作用,其准确估算对基于CSLE模型的区域土壤侵蚀评价及动态对比分析至关重要。通过文献资料调研,总结目前获取T因子的6种方法,并对比各种方法的优缺点和适用条件。研究表明:水利普查结果查询法和区域水土流失动态监测查询法,获取便捷且应用广泛,但精度有待验证;土地利用类型划分公式法计算简便且适用于流域等大尺度,但受土地利用/覆盖类型解译的限制;径流小区公式法精度较高,适用于小区等小尺度;坡度分级法和水土保持耕作措施赋值法实际应用性强,可用于小流域、区域等尺度,但需考虑空间异质性。展开更多
基金supported by the China Postdoctoral Science Foundation (No. 2019M653152)the Pearl River S&T Nova Program of Guangzhou (No. 201806010047)+2 种基金the National Natural Science Foundation of China (No. 31771587)Fundamental Research Funds for the Central Universities (No. 19lgpy102)the Natural Science Foundation of Guangdong Province (No. 2019A1515110842)
文摘Atlantic salmon(Salmo salar)is an important economic fish that is seriously threatened by various viruses.A cell line designated as ASF derived from the caudal fin tissue of Atlantic salmon was established and characterized in this study.ASF cells grew well in Dulbecco’s modified Eagle’s medium(DMEM)containing 20%fetal bovine serum at 20℃.DNA sequencing and comparative analysis of the cytochrome B gene verified that the ASF cell line originated from Atlantic salmon.Chromosome analysis indicated that the modal chromosome number of ASF cells was 58.Viral susceptibility test showed that ASF cells were susceptive to two important fish viruses,viral hemorrhagic septicemia virus(VHSV)and red-spotted grouper nervous necrosis virus(RGNNV).Viral replication in ASF cells was further confirmed by qRT-PCR and transmission electron microscopy.Moreover,VHSV and RGNNV infections could induce the cellular responses in ASF cells,as indicated by the differential expression of cellular antiviral response-related genes,interferon-1 and Mx-1.In conclusion,the newly established ASF cell line can be applied as an in vitro tool in fish virology and immunity studies.
文摘目的通过研究哺乳动物雷帕霉素靶蛋白(mammalian target of rapamycin,mTOR)与结直肠癌的生物信息,意在推动结直肠癌医疗手段进步,实现挽救结直肠癌患者生命的目的。方法分析2013年5月—2021年5月癌症基因组图谱(the cancer genome atlas,TCGA)公共数据库的COAD&READ患者数据698份,其中正常组51例,结肿瘤组647份。分析了TCGA结直肠癌患者的表达谱、mTOR的表达和生存差异,并探讨了mTOR的共表达调控网络。此外,还探讨了mTOR与富集通路、微卫星不稳定性(microsatellite instability,MSI)、肿瘤突变负荷(tumor mutation burden,TMB)的关系。结果基于GSE73360数据库分析mTOR在组织中的表达差异,mTOR在正常组织中表达(7.0084±0.429089872)低于mTOR在肿瘤组织中表达(8.04058±0.784400233),差异有统计学意义(P<0.05)。共筛选出541个与mTOR表达显著相关的基因。核心基因高低表达在MSI和TMB方面比较,差异有统计学意义(P<0.05)。结论mTOR可以作为结直肠癌诊断和预后的生物标志物。
文摘中国土壤流失方程(Chinese soil loss equation,CSLE)是我国用于定量评价和预测区域土壤侵蚀的重要模型。水土保持耕作措施因子(T)是该模型中的7个关键因子之一,能反映水土保持耕作措施对土壤侵蚀的削减作用,其准确估算对基于CSLE模型的区域土壤侵蚀评价及动态对比分析至关重要。通过文献资料调研,总结目前获取T因子的6种方法,并对比各种方法的优缺点和适用条件。研究表明:水利普查结果查询法和区域水土流失动态监测查询法,获取便捷且应用广泛,但精度有待验证;土地利用类型划分公式法计算简便且适用于流域等大尺度,但受土地利用/覆盖类型解译的限制;径流小区公式法精度较高,适用于小区等小尺度;坡度分级法和水土保持耕作措施赋值法实际应用性强,可用于小流域、区域等尺度,但需考虑空间异质性。
