目的:基于Web of Science血液净化临床护理研究的文献进行可视化分析。方法:检索Web of Science数据库核心合集中1993—2022年收录的血液净化临床护理的相关研究;使用CiteSpace 6.2.R2软件对该研究领域的作者、国家、关键词等进行可视...目的:基于Web of Science血液净化临床护理研究的文献进行可视化分析。方法:检索Web of Science数据库核心合集中1993—2022年收录的血液净化临床护理的相关研究;使用CiteSpace 6.2.R2软件对该研究领域的作者、国家、关键词等进行可视化分析。结果:共纳入2164篇文献,可视化分析显示相关研究发文量呈波动上升趋势,发文量居前5位的国家分别是美国(704篇)、中国(203篇)、澳大利亚(197篇)、加拿大(165篇)和巴西(165篇);多伦多大学发文量最多,发文最多的作者是来自澳大利亚格里菲斯大学的Bennett P N。研究热点主要集中在生活质量、血液透析、慢性肾脏病、死亡率等方面。结论:血液净化临床护理研究的发文量呈逐年上升趋势,研究热点主要集中于血液净化在临床护理的应用和影响;本研究结果可帮助研究者更准确、具体地了解该领域现状,并及时追踪其研究动向。展开更多
Objective： To study the expression of osteosarcoma metastasis associated gene using a cDNA microarray, and screen new candidate genes related＇to the development, progress and osteosarcoma metastasis. Methods： Total...Objective： To study the expression of osteosarcoma metastasis associated gene using a cDNA microarray, and screen new candidate genes related＇to the development, progress and osteosarcoma metastasis. Methods： Total RNA of a low metastatic osteosarcoma and a high metastatic osteosarcoma （M6 and M8 cell lines, respectively） was extracted, purified to mRNA and then reverse transcribed to cDNA. M6 was used as the experimental group and M8 as the control group, and the gene expression of cells from both of these two sublines was investigated using cDNA microarrays containig 8064 cDNA clones. The cDNA of M6 was labeled with cy3 and the cDNA of M8 was labeled with cyS. The two sublines were hybridized with the cDNA microarray. The hybridization signals were scanned with a Generation HI array scanner and analyzed by Imagequant 5.0 software. Results： There were 330 differentially expressed genes between M6 and M8. In the M6 subline,152 genes were up-regulated and 178 genes were down-regulated compared to the M8 subline. These genes could be classified according to their function. Cell growth-related genes that were down-regulated included CCNG1, CDC2, APC10,and RPA3, while expression of the tumor suppressor genes, CDKN1A and CDKN2D, was up-regulated. Other genes that were differentially expressed included those that have been implicated in the regulation of signal transduction, metabolism and apoptosis. Conclusion： This study exploits a cDNA microarray approach to identifying genes that may be associated with metastasis. The gene expression profiles of osteosarcoma cell lines is a potentially important index in the search of new candidate genes related to tumor occurrence, development and metastasis.展开更多
目的利用CiteSpace文献计量学软件,本文对KM小鼠的研究现状和热点进行了可视化分析,旨在为国内KM小鼠研究的突破和创新提供依据。方法本文基于中国知网和Web of Science两个数据库,纳入了2004年1月1日~2023年6月10日的3981篇KM小鼠相关...目的利用CiteSpace文献计量学软件,本文对KM小鼠的研究现状和热点进行了可视化分析,旨在为国内KM小鼠研究的突破和创新提供依据。方法本文基于中国知网和Web of Science两个数据库,纳入了2004年1月1日~2023年6月10日的3981篇KM小鼠相关文献,分析了其发文量、国家(地区)、机构、作者合作网络、关键词共现、聚类、突现词和英文文献被引情况。结果KM小鼠研究中文文献发文量波动下降,英文文献发文量逐渐上升,国内外对于KM小鼠的研究热度不同。发文最多的3个国家是中国、美国和日本,国内的主要研究机构有中国农业大学、南京中医药大学以及中国科学院等,发文量较多的作者主要是刘慧雯、李光武和赵欣等。国内期刊研究内容主要集中于KM小鼠毒理学、免疫学和生殖学方面,国外期刊研究主要集中于KM小鼠细胞生物学、生理学方面。结论目前,KM小鼠在我国已有80余年的应用与推广历史,主要集中在毒理学、免疫学和生殖学方面研究。KM小鼠虽在实验方面有一定成果,但规范化种群培育、优势研究领域发掘以及动物伦理的进一步推进是未来需要继续解决的问题。展开更多
Electrocatalytic converting CO_(2) into chemical products has emerged as a promising approach to achieving carbon neutrality.Herein,we report a bismuth-based catalyst with high curvature terminal and amorphous layer w...Electrocatalytic converting CO_(2) into chemical products has emerged as a promising approach to achieving carbon neutrality.Herein,we report a bismuth-based catalyst with high curvature terminal and amorphous layer which fabricated via two-step electrodeposition achieves stable formate output in a wide voltage window of 600 mV.The Faraday efficiency(FE) of formate reached up to 99.4% at-0.8 V vs.RHE and it remained constant for more than 92 h at-15 mA cm^(-2).More intriguingly,FE formate of95.4% can be realized at a current density of industrial grade(-667.7 mA cm^(-2)) in flow cell.The special structure promoted CO_(2) adsorption and reduced its activation energy and enhanced the electric-thermal field and K^(+) enrichment which accelerated the reaction kinetics.In situ spectroscopy and theoretical calculation further confirmed that the introduction of amorphous structure is beneficial to adsorpting CO_(2)and stabling*OCHO intermediate.This work provides special insights to fabricate efficient electrocatalysts by means of structural and crystal engineering and makes efforts to realize the industrialization of bismuth-based catalysts.展开更多
Ensuring a stable power output from renewable energy sources,such as wind and solar energy,depends on the development of large-scale and long-duration energy storage devices.Zinc–bromine fl ow batteries(ZBFBs)have em...Ensuring a stable power output from renewable energy sources,such as wind and solar energy,depends on the development of large-scale and long-duration energy storage devices.Zinc–bromine fl ow batteries(ZBFBs)have emerged as cost-eff ective and high-energy-density solutions,replacing expensive all-vanadium fl ow batteries.However,uneven Zn deposition during charging results in the formation of problematic Zn dendrites,leading to mass transport polarization and self-discharge.Stable Zn plating and stripping are essential for the successful operation of high-areal-capacity ZBFBs.In this study,we successfully synthesized nitrogen and oxygen co-doped functional carbon felt(NOCF4)electrode through the oxidative polymerization of dopamine,followed by calcination under ambient conditions.The NOCF4 electrode eff ectively facilitates effi cient“shuttle deposition”of Zn during charging,signifi cantly enhancing the areal capacity of the electrode.Remarkably,ZBFBs utilizing NOCF4 as the anode material exhibited stable cycling performance for 40 cycles(approximately 240 h)at an areal capacity of 60 mA h/cm^(2).Even at a high areal capacity of 130 mA h/cm^(2),an impressive energy effi ciency of 76.98%was achieved.These fi ndings provide a promising pathway for the development of high-areal-capacity ZBFBs for advanced energy storage systems.展开更多
Osteogenesis imperfecta(OI)is a disorder of low bone mass and increased fracture risk due to a range of genetic variants that prominently include mutations in genes encoding typeⅠcollagen.While it is well known that ...Osteogenesis imperfecta(OI)is a disorder of low bone mass and increased fracture risk due to a range of genetic variants that prominently include mutations in genes encoding typeⅠcollagen.While it is well known that OI reflects defects in the activity of bone-forming osteoblasts,it is currently unclear whether OI also reflects defects in the many other cell types comprising bone,including defects in skeletal vascular endothelium or the skeletal stem cell populations that give rise to osteoblasts and whether correcting these broader defects could have therapeutic utility.展开更多
文摘目的:基于Web of Science血液净化临床护理研究的文献进行可视化分析。方法:检索Web of Science数据库核心合集中1993—2022年收录的血液净化临床护理的相关研究;使用CiteSpace 6.2.R2软件对该研究领域的作者、国家、关键词等进行可视化分析。结果:共纳入2164篇文献,可视化分析显示相关研究发文量呈波动上升趋势,发文量居前5位的国家分别是美国(704篇)、中国(203篇)、澳大利亚(197篇)、加拿大(165篇)和巴西(165篇);多伦多大学发文量最多,发文最多的作者是来自澳大利亚格里菲斯大学的Bennett P N。研究热点主要集中在生活质量、血液透析、慢性肾脏病、死亡率等方面。结论:血液净化临床护理研究的发文量呈逐年上升趋势,研究热点主要集中于血液净化在临床护理的应用和影响;本研究结果可帮助研究者更准确、具体地了解该领域现状,并及时追踪其研究动向。
文摘Objective： To study the expression of osteosarcoma metastasis associated gene using a cDNA microarray, and screen new candidate genes related＇to the development, progress and osteosarcoma metastasis. Methods： Total RNA of a low metastatic osteosarcoma and a high metastatic osteosarcoma （M6 and M8 cell lines, respectively） was extracted, purified to mRNA and then reverse transcribed to cDNA. M6 was used as the experimental group and M8 as the control group, and the gene expression of cells from both of these two sublines was investigated using cDNA microarrays containig 8064 cDNA clones. The cDNA of M6 was labeled with cy3 and the cDNA of M8 was labeled with cyS. The two sublines were hybridized with the cDNA microarray. The hybridization signals were scanned with a Generation HI array scanner and analyzed by Imagequant 5.0 software. Results： There were 330 differentially expressed genes between M6 and M8. In the M6 subline,152 genes were up-regulated and 178 genes were down-regulated compared to the M8 subline. These genes could be classified according to their function. Cell growth-related genes that were down-regulated included CCNG1, CDC2, APC10,and RPA3, while expression of the tumor suppressor genes, CDKN1A and CDKN2D, was up-regulated. Other genes that were differentially expressed included those that have been implicated in the regulation of signal transduction, metabolism and apoptosis. Conclusion： This study exploits a cDNA microarray approach to identifying genes that may be associated with metastasis. The gene expression profiles of osteosarcoma cell lines is a potentially important index in the search of new candidate genes related to tumor occurrence, development and metastasis.
文摘目的利用CiteSpace文献计量学软件,本文对KM小鼠的研究现状和热点进行了可视化分析,旨在为国内KM小鼠研究的突破和创新提供依据。方法本文基于中国知网和Web of Science两个数据库,纳入了2004年1月1日~2023年6月10日的3981篇KM小鼠相关文献,分析了其发文量、国家(地区)、机构、作者合作网络、关键词共现、聚类、突现词和英文文献被引情况。结果KM小鼠研究中文文献发文量波动下降,英文文献发文量逐渐上升,国内外对于KM小鼠的研究热度不同。发文最多的3个国家是中国、美国和日本,国内的主要研究机构有中国农业大学、南京中医药大学以及中国科学院等,发文量较多的作者主要是刘慧雯、李光武和赵欣等。国内期刊研究内容主要集中于KM小鼠毒理学、免疫学和生殖学方面,国外期刊研究主要集中于KM小鼠细胞生物学、生理学方面。结论目前,KM小鼠在我国已有80余年的应用与推广历史,主要集中在毒理学、免疫学和生殖学方面研究。KM小鼠虽在实验方面有一定成果,但规范化种群培育、优势研究领域发掘以及动物伦理的进一步推进是未来需要继续解决的问题。
基金financial support from the Zhejiang Provincial Natural Science Foundation of China(LQ22B060007)the National Natural Science Foundation of China(22206042)+2 种基金the Scientific Research Start-up of Hangzhou Normal University(2021GDL014)the Hebei Natural Science Foundation(E2021203047)the Hebei Provincial Foundation for Returness(C20200369)。
文摘Electrocatalytic converting CO_(2) into chemical products has emerged as a promising approach to achieving carbon neutrality.Herein,we report a bismuth-based catalyst with high curvature terminal and amorphous layer which fabricated via two-step electrodeposition achieves stable formate output in a wide voltage window of 600 mV.The Faraday efficiency(FE) of formate reached up to 99.4% at-0.8 V vs.RHE and it remained constant for more than 92 h at-15 mA cm^(-2).More intriguingly,FE formate of95.4% can be realized at a current density of industrial grade(-667.7 mA cm^(-2)) in flow cell.The special structure promoted CO_(2) adsorption and reduced its activation energy and enhanced the electric-thermal field and K^(+) enrichment which accelerated the reaction kinetics.In situ spectroscopy and theoretical calculation further confirmed that the introduction of amorphous structure is beneficial to adsorpting CO_(2)and stabling*OCHO intermediate.This work provides special insights to fabricate efficient electrocatalysts by means of structural and crystal engineering and makes efforts to realize the industrialization of bismuth-based catalysts.
基金supported by Natural Science Foundation of Anhui Higher Education Institution of China(2023AH051318).
文摘Ensuring a stable power output from renewable energy sources,such as wind and solar energy,depends on the development of large-scale and long-duration energy storage devices.Zinc–bromine fl ow batteries(ZBFBs)have emerged as cost-eff ective and high-energy-density solutions,replacing expensive all-vanadium fl ow batteries.However,uneven Zn deposition during charging results in the formation of problematic Zn dendrites,leading to mass transport polarization and self-discharge.Stable Zn plating and stripping are essential for the successful operation of high-areal-capacity ZBFBs.In this study,we successfully synthesized nitrogen and oxygen co-doped functional carbon felt(NOCF4)electrode through the oxidative polymerization of dopamine,followed by calcination under ambient conditions.The NOCF4 electrode eff ectively facilitates effi cient“shuttle deposition”of Zn during charging,signifi cantly enhancing the areal capacity of the electrode.Remarkably,ZBFBs utilizing NOCF4 as the anode material exhibited stable cycling performance for 40 cycles(approximately 240 h)at an areal capacity of 60 mA h/cm^(2).Even at a high areal capacity of 130 mA h/cm^(2),an impressive energy effi ciency of 76.98%was achieved.These fi ndings provide a promising pathway for the development of high-areal-capacity ZBFBs for advanced energy storage systems.
基金supported by the National Natural Science Foundation of China (81972034,92068104 and 82002262 to R.X.)the National Key R&D Program of China (2020YFA0112900 to R.X.)+5 种基金Project of Xiamen Cell Therapy Research Center (3502Z20214001 to R.X.)supported by a the NIH grant of US (R01AR075585,R01HD115274,R01CA282815 to M.B.G.)Career Award for Medical Scientists from the Burroughs Wellcome Funda Pershing Square Sohn Cancer Research Alliance and the Maximizing Innovation in Neuroscience Discovery (MIND)Prizesupported by a Jump Start Research Career Development Award from Weill Cornell Medicinea Study Abroad Scholarships from the Mogam Science Scholarship Foundation。
文摘Osteogenesis imperfecta(OI)is a disorder of low bone mass and increased fracture risk due to a range of genetic variants that prominently include mutations in genes encoding typeⅠcollagen.While it is well known that OI reflects defects in the activity of bone-forming osteoblasts,it is currently unclear whether OI also reflects defects in the many other cell types comprising bone,including defects in skeletal vascular endothelium or the skeletal stem cell populations that give rise to osteoblasts and whether correcting these broader defects could have therapeutic utility.
